Overview
- External ontology
- mediadive.medium:J70 (source link unavailable) — BLOOD AGAR
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=70
Composition
flowchart LR
CultureMech_003056["blood_agar"]
ing0_Heart_muscle_infusion_solids["Heart muscle infusion solids"]
CultureMech_003056 --> ing0_Heart_muscle_infusion_solids
ing1_Pancreatic_digest_of_casein["Pancreatic digest of casein"]
CultureMech_003056 --> ing1_Pancreatic_digest_of_casein
ing2_Yeast_extract["Yeast extract"]
CultureMech_003056 --> ing2_Yeast_extract
ing3_Sodium_chloride["Sodium chloride"]
CultureMech_003056 --> ing3_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing3_Sodium_chloride -.-> CHEBI_26710
ing4_Agar["Agar"]
CultureMech_003056 --> ing4_Agar
CHEBI_2509["CHEBI:2509"]
ing4_Agar -.-> CHEBI_2509
ing5_Defibrinated_rabbit_blood["Defibrinated rabbit blood"]
CultureMech_003056 --> ing5_Defibrinated_rabbit_blood
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Heart muscle infusion solids | 2.0 G_PER_L | From BD-Difco Blood Agar Base (211037); Infusion from heart muscle - provides vitamins, nitrogen, carbon | |
| 2 | Pancreatic digest of casein | 13.0 G_PER_L |
MICRO:0000182 (source link unavailable)
tryptone
|
From BD-Difco Blood Agar Base (211037); Nitrogen and amino acid source |
| 3 | Yeast extract | 5.0 G_PER_L |
FOODON:03315426
yeast extract
|
From BD-Difco Blood Agar Base (211037); Vitamin and amino acid source |
| 4 |
Sodium chloride
Cl.Na 58.443 g/mol
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
From BD-Difco Blood Agar Base (211037); Maintains osmotic equilibrium |
| 5 | Agar | 15.0 G_PER_L |
CHEBI:2509
agar
|
From BD-Difco Blood Agar Base (211037); Solidifying agent |
| 6 | Defibrinated rabbit blood | 5 PERCENT_V_V |
UBERON:0000178
blood
|
Added aseptically after autoclaving and cooling to 45°C; 5% final concentration |
Preparation
- Prepare Blood agar base (BD-Difco) according to the direction, sterilize and cool to about 45C. Aseptically add final 5% sterile defibrinated rabbit blood. Mix and quickly dispense into sterile test tubes.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- blood_agar (CultureMech:003056)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 0 re-grounded to the correct CHEBI, 1 de-grounded (mixture or unverifiable). |
| 2026-01-26T17:53:15.680102Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J70 |
| 2026-02-03T11:26:55.818678 | quality-tagger-v1.0 | Added data quality flag | Marked as incomplete_composition due to placeholder ingredients |
| 2026-03-13T06:19:17.476406Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003056 |
| 2026-03-17T19:45:31.188093+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BLOOD AGAR" to "blood_agar" for consistent matching |
| 2026-04-05T12:55:23.800036 | expand_blood_agar | EXPANDED_COMMERCIAL_PRODUCT | Commercial product expansion: BD-Difco Blood Agar Base (Infusion Agar) with rabbit blood |
| 2026-05-16T08:57:24.599978+00:00 | migrate_data_quality_flags.py | MIGRATED_DATA_QUALITY_FLAGS | dict(4 keys) -> list(3 entries) |
| 2026-08-06T03:05:25.606427+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 45bfc12d73e50979e2254eda907d95b0a172e8a525bd53b82f5c77c343519e92 |