Overview
- External ontology
- mediadive.medium:J741 (source link unavailable) — ACETOBACTERIUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=741
Composition
flowchart LR
CultureMech_003084["acetobacterium_medium"]
ing0_NH4Cl["NH4Cl"]
CultureMech_003084 --> ing0_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing0_NH4Cl -.-> CHEBI_31206
ing1_KH2PO4["KH2PO4"]
CultureMech_003084 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_003084 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003084 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_Yeast_extract["Yeast extract"]
CultureMech_003084 --> ing4_Yeast_extract
ing5_Fructose["Fructose"]
CultureMech_003084 --> ing5_Fructose
CHEBI_28757["CHEBI:28757"]
ing5_Fructose -.-> CHEBI_28757
ing6_Resazurin["Resazurin"]
CultureMech_003084 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_NaHCO3["NaHCO3"]
CultureMech_003084 --> ing7_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing7_NaHCO3 -.-> CHEBI_32139
ing8_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003084 --> ing8_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing8_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003084 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003084 --> ing10_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing10_Nitrilotriacetic_acid -.-> CHEBI_44557
ing11_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003084 --> ing11_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing11_MnSO4_x_n_H2O -.-> CHEBI_86360
ing12_NaCl["NaCl"]
CultureMech_003084 --> ing12_NaCl
CHEBI_26710["CHEBI:26710"]
ing12_NaCl -.-> CHEBI_26710
ing13_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003084 --> ing13_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing13_FeSO4_x_7_H2O -.-> CHEBI_75836
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003084 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003084 --> ing15_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003084 --> ing16_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003084 --> ing17_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
ing18_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
CultureMech_003084 --> ing18_AlK_SO4_2_x_12_H2O
CHEBI_86465["CHEBI:86465"]
ing18_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
ing19_H3BO3["H3BO3"]
CultureMech_003084 --> ing19_H3BO3
CHEBI_33118["CHEBI:33118"]
ing19_H3BO3 -.-> CHEBI_33118
ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003084 --> ing20_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing21_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003084 --> ing21_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing21_NiCl2_x_6_H2O -.-> CHEBI_34887
ing22_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003084 --> ing22_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing22_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing23_Biotin["Biotin"]
CultureMech_003084 --> ing23_Biotin
CHEBI_15956["CHEBI:15956"]
ing23_Biotin -.-> CHEBI_15956
ing24_Folic_acid["Folic acid"]
CultureMech_003084 --> ing24_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing24_Folic_acid -.-> CHEBI_27470
ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003084 --> ing25_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing26_Thiamine_HCl["Thiamine HCl"]
CultureMech_003084 --> ing26_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing26_Thiamine_HCl -.-> CHEBI_49105
ing27_Riboflavin["Riboflavin"]
CultureMech_003084 --> ing27_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing27_Riboflavin -.-> CHEBI_17015
ing28_Nicotinic_acid["Nicotinic acid"]
CultureMech_003084 --> ing28_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing28_Nicotinic_acid -.-> CHEBI_15940
ing29_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003084 --> ing29_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing29_Calcium_pantothenate -.-> CHEBI_31345
CultureMech_003084 --> more["...3 more"]
Ingredients (33)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.961538 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.317308 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.432692 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3.0961538 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
[Merged 2 duplicates: 0.0961538, 3.0] |
| 5 | Yeast extract | 1.92308 G_PER_L | ||
| 6 | Fructose | 9.61539 G_PER_L |
CHEBI:28757
fructose
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000961538 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
NaHCO3
CHO3.Na 84.006 g/mol
|
9.61539 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 9 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.480769 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.480769 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 12 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 13 |
NaCl
Cl.Na 58.443 g/mol
|
1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 14 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.18 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 16 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 17 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.18 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 18 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 19 |
AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
|
0.02 G_PER_L |
CHEBI:86465
potassium aluminium sulfate dodecahydrate
|
|
| 20 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 21 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 22 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.025 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 23 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.0003 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 24 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 25 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 26 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 27 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 28 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 29 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 30 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 31 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 32 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 33 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Dissolve ingredients except NaHCO3, fructose, cysteine and sodium sulfide, bring to a boil for a few minutes and cool to room temperature under a N2-CO2 (80:20, v/v) gas mixture. Add NaHCO3 (solid) and equilibrate the medium with the gas until pH reaches ca. 7.4. Then distribute and autoclave under the same gas atmosphere. Before use, adjust pH to 8.2 by adding sterile anaerobic Na2CO3 solution (ca. 0.25 ml of 5% Na2CO3 per 10 ml medium) and add fructose, cysteine and sodium sulfide from anaerobic sterile stock solutions.
- Dissolve nitrilotriacetic acid at first and adjust pH to 6.5 with KOH, then add the remaining minerals.
- Adjust final pH of the medium to 7.0 with KOH.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J741_ACETOBACTERIUM_MEDIUM (CultureMech:003084)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.450226Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J741 |
| 2026-03-13T03:09:00.255915Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:17.672086Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003084 |
| 2026-03-17T06:30:05.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.418216+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACETOBACTERIUM MEDIUM" to "acetobacterium_medium" for consistent matching |
| 2026-06-05T07:40:17.659440+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 31 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.088173+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:37.200328+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 50ec0c4aef854b406856b23792c9721941c2859c62ca6d4d42990263f05eaebd |