modified_hb_1_medium

CultureMech:003087 bacterial DEFINED LIQUID
Original name: MODIFIED HB-1 MEDIUM

Overview

External ontology
mediadive.medium:J744 (source link unavailable) — MODIFIED HB-1 MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=744

Composition

flowchart LR
    CultureMech_003087["modified_hb_1_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003087 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003087 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003087 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_003087 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NaBr["NaBr"]
    CultureMech_003087 --> ing4_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing4_NaBr -.-> CHEBI_63004
    ing5_H3BO3["H3BO3"]
    CultureMech_003087 --> ing5_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing5_H3BO3 -.-> CHEBI_33118
    ing6_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_003087 --> ing6_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing6_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing7__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003087 --> ing7__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing7__NH4_2SO4 -.-> CHEBI_62946
    ing8_KI["KI"]
    CultureMech_003087 --> ing8_KI
    CHEBI_8346["CHEBI:8346"]
    ing8_KI -.-> CHEBI_8346
    ing9_Na2WO2_x_2_H2O["Na2WO2 x 2 H2O"]
    CultureMech_003087 --> ing9_Na2WO2_x_2_H2O
    ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003087 --> ing10_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing11_KH2PO4["KH2PO4"]
    CultureMech_003087 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003087 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_Trace_minerals__see_Medium_No__151["Trace minerals (see Medium No. 151"]
    CultureMech_003087 --> ing13_Trace_minerals__see_Medium_No__151
    ing14_Resazurin["Resazurin"]
    CultureMech_003087 --> ing14_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing14_Resazurin -.-> CHEBI_8806
    ing15_KNO3["KNO3"]
    CultureMech_003087 --> ing15_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing15_KNO3 -.-> CHEBI_63043
    ing16_PIPES["PIPES"]
    CultureMech_003087 --> ing16_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing16_PIPES -.-> CHEBI_39033
    ing17_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003087 --> ing17_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing17_Na2S_x_9_H2O -.-> CHEBI_76209

Ingredients (18)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
19.0658 G_PER_L CHEBI:26710
sodium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3.33651 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
2.62154 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
4 KCl
Cl.K 74.551 g/mol
0.309819 G_PER_L CHEBI:32588
potassium chloride
5 NaBr
Br.Na 102.894 g/mol
0.0476644 G_PER_L CHEBI:63004
sodium bromide
6 H3BO3
H3BO3 61.833 g/mol
0.0142993 G_PER_L CHEBI:33118
boric acid
7 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.00714967 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
8 (NH4)2SO4
2H4N.O4S 132.141 g/mol
0.00953289 G_PER_L CHEBI:62946
ammonium sulfate
9 KI
I.K 166.002 g/mol
0.000476644 G_PER_L CHEBI:8346
potassium iodide
10 Na2WO2 x 2 H2O 9.53289e-05 G_PER_L
11 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.714967 G_PER_L CHEBI:86158
calcium chloride dihydrate
12 KH2PO4
H2O4P.K 136.084 g/mol
0.476644 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.00190658 G_PER_L CHEBI:34887
nickel dichloride
14 Trace minerals (see Medium No. 151 10 G_PER_L
15 Resazurin
C12H7NO4 229.191 g/mol
0.000953289 G_PER_L CHEBI:8806
Resazurin
16 KNO3
K.NO3 101.102 g/mol
10 G_PER_L CHEBI:63043
potassium nitrate
17 PIPES 25 G_PER_L CHEBI:39033
PIPES
18 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
4 G_PER_L CHEBI:76209
sodium sulfide nonahydrate

Preparation

  1. Mix ingredients, bring to a boil and cool under a H2-CO2 (4:1, v/v) gas mixture. Slowly add 10 ml 5% Na2S x 9H2O solution to the medium, wait for the medium to become colorless, then adjust pH to 7.0 with NaOH. Distribute medium into culture vessels under the same gas mixture, seal with butyl rubber stoppers and autoclave. Before inoculation, aseptically and anaerobically add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 atmosphere):
  2. Pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.462049Z mediadive-import Imported from MediaDive Source: JCM, ID: J744
2026-03-13T06:19:17.702386Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003087
2026-03-17T06:29:48.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.452955+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED HB-1 MEDIUM" to "modified_hb_1_medium" for consistent matching
2026-06-05T07:41:36.711962+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.634418+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:08.658978+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:48.386308+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint abb5c4baa0160312d38ff21c256419b3314bc3934236f20662b62e2b0eb551bb