metallosphaera_cuprina_medium

CultureMech:003091 archaea COMPLEX LIQUID
Original name: METALLOSPHAERA CUPRINA MEDIUM

Overview

External ontology
mediadive.medium:J748 (source link unavailable) — METALLOSPHAERA CUPRINA MEDIUM
Applications
  • Microbial cultivation
pH
3.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=748

Composition

flowchart LR
    CultureMech_003091["metallosphaera_cuprina_medium"]
    ing0__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003091 --> ing0__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing0__NH4_2SO4 -.-> CHEBI_62946
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003091 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003091 --> ing2_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing3_KCl["KCl"]
    CultureMech_003091 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_Ca_NO3_2["Ca(NO3)2"]
    CultureMech_003091 --> ing4_Ca_NO3_2
    CHEBI_64205["CHEBI:64205"]
    ing4_Ca_NO3_2 -.-> CHEBI_64205
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003091 --> ing5_Yeast_extract
    ing6_Tryptone["Tryptone"]
    CultureMech_003091 --> ing6_Tryptone
    ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003091 --> ing7_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing8_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003091 --> ing8_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing8_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing9_H3BO3["H3BO3"]
    CultureMech_003091 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003091 --> ing10_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing10_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003091 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003091 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003091 --> ing13_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing13_ZnSO4_x_7_H2O -.-> CHEBI_32312

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 (NH4)2SO4
2H4N.O4S 132.141 g/mol
3 G_PER_L CHEBI:62946
ammonium sulfate
2 KH2PO4
H2O4P.K 136.084 g/mol
0.5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
4 KCl
Cl.K 74.551 g/mol
0.1 G_PER_L CHEBI:32588
potassium chloride
5 Ca(NO3)2
Ca.2NO3 164.086 g/mol
0.01 G_PER_L CHEBI:64205
calcium nitrate
6 Yeast extract 2 G_PER_L
7 Tryptone 0.5 G_PER_L
8 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
1.1 G_PER_L CHEBI:86254
iron trichloride hexahydrate
9 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.05 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
10 H3BO3
H3BO3 61.833 g/mol
0.2 G_PER_L CHEBI:33118
boric acid
11 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.2 G_PER_L CHEBI:86360
manganese(II) sulfate
12 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.08 G_PER_L CHEBI:75213
sodium molybdate dihydrate
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.06 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.09 G_PER_L CHEBI:32312
zinc sulfate heptahydrate

Preparation

  1. Adjust pH to 3.5 and autoclave. After cooling, aseptically add 2.0 ml of Trace element solution (see below).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.474383Z mediadive-import Imported from MediaDive Source: JCM, ID: J748
2026-02-06T05:10:54.350854Z invalid-chebi-removal Removed 1 invalid CHEBI ID(s) Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data
2026-03-13T06:19:17.735136Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003091
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.490528+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METALLOSPHAERA CUPRINA MEDIUM" to "metallosphaera_cuprina_medium" for consistent matching
2026-06-05T07:41:30.521735+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:47.623004+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:06.594097+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:29.392415+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 36e15decaad6aeeba136148cfba1d9b87bba320fb020f9a5f30be05f642bcb77