modified_nautilia_lithotrophica_medium

CultureMech:003094 bacterial DEFINED LIQUID
Original name: MODIFIED NAUTILIA LITHOTROPHICA MEDIUM

Overview

External ontology
mediadive.medium:J751 (source link unavailable) — MODIFIED NAUTILIA LITHOTROPHICA MEDIUM
Applications
  • Microbial cultivation
pH
6.8
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=751

Composition

flowchart LR
    CultureMech_003094["modified_nautilia_lithotrophica_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003094 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_NaNO3["NaNO3"]
    CultureMech_003094 --> ing1_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing1_NaNO3 -.-> CHEBI_63005
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003094 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KCl["KCl"]
    CultureMech_003094 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003094 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003094 --> ing5_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing6_HEPES["HEPES"]
    CultureMech_003094 --> ing6_HEPES
    CHEBI_46756["CHEBI:46756"]
    ing6_HEPES -.-> CHEBI_46756
    ing7_Resazurin["Resazurin"]
    CultureMech_003094 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Sulfur["Sulfur"]
    CultureMech_003094 --> ing8_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing8_Sulfur -.-> CHEBI_26833
    ing9_Sodium_formate["Sodium formate"]
    CultureMech_003094 --> ing9_Sodium_formate
    CHEBI_62965["CHEBI:62965"]
    ing9_Sodium_formate -.-> CHEBI_62965
    ing10_NaHCO3["NaHCO3"]
    CultureMech_003094 --> ing10_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing10_NaHCO3 -.-> CHEBI_32139
    ing11_Trace_vitamins__see_Medium_No__197_["Trace vitamins (see Medium No. 197)"]
    CultureMech_003094 --> ing11_Trace_vitamins__see_Medium_No__197_
    ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003094 --> ing12_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing12_Na2S_x_9_H2O -.-> CHEBI_76209
    ing13_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003094 --> ing13_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing13_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003094 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003094 --> ing15_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing15_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003094 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003094 --> ing17_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing17_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003094 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003094 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003094 --> ing20_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing20_AlK_SO4_2 -.-> CHEBI_86463
    ing21_H3BO3["H3BO3"]
    CultureMech_003094 --> ing21_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing21_H3BO3 -.-> CHEBI_33118
    ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003094 --> ing22_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing23_NaOH["NaOH"]
    CultureMech_003094 --> ing23_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing23_NaOH -.-> CHEBI_32145
    ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003094 --> ing24_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003094 --> ing25_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939

Ingredients (26)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
25.728 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 24.728, 1.0]
2 NaNO3
NO3.Na 84.994 g/mol
0.98912 G_PER_L CHEBI:63005
sodium nitrate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.326409 G_PER_L CHEBI:31206
ammonium chloride
4 KCl
Cl.K 74.551 g/mol
0.326409 G_PER_L CHEBI:32588
potassium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.42640900000000004 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.326409, 0.1]
6 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.326409 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
7 HEPES 2.3541 G_PER_L CHEBI:46756
HEPES
8 Resazurin
C12H7NO4 229.191 g/mol
0.00098912 G_PER_L CHEBI:8806
Resazurin
9 Sulfur
S 32.067 g/mol
11.8694 G_PER_L CHEBI:26833
sulfur atom
10 Sodium formate
CHO2.Na 68.007 g/mol
20 G_PER_L CHEBI:62965
sodium formate
11 NaHCO3
CHO3.Na 84.006 g/mol
50 G_PER_L CHEBI:32139
sodium hydrogencarbonate
12 Trace vitamins (see Medium No. 197) 10 G_PER_L
13 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
20 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
14 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
15 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
16 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
22 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
23 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
24 NaOH
HO.Na 39.997 g/mol
0.4 G_PER_L CHEBI:32145
sodium hydroxide
25 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.006 G_PER_L CHEBI:131361
disodium selenite pentahydrate
26 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.008 G_PER_L CHEBI:63939
sodium tungstate dihydrate

Preparation

  1. Mix components except sulfur, adjust pH to 6.8 and autoclave under a N2-CO2 (4:1, v/v) atmosphere. Distribute medium into culture vessels (e.g., 18 ml in 100 ml serum bottles) containing appropriate amount of sulfur under a N2-CO2 (4:1, v/v) gas mixture, and seal with butyl rubber stoppers. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) to the above described basal medium (900 ml):
  2. Readjust pH of the medium to 6.8, if necessary.
  3. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.483481Z mediadive-import Imported from MediaDive Source: JCM, ID: J751
2026-03-13T03:09:42.038196Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:17.761212Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003094
2026-03-17T06:30:54.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.522195+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED NAUTILIA LITHOTROPHICA MEDIUM" to "modified_nautilia_lithotrophica_medium" for consistent matching
2026-06-05T07:41:37.476432+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:08.864453+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:49.245978+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 34d888303f9184ff6e1052661e0853b74288fa4e3a3cbf985c255f883c97aba7