Overview
- External ontology
- mediadive.medium:J754 (source link unavailable) — MJ MEDIUM FOR DEFFERIBACTER SP. STRAIN NEJINEJI
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=754
Composition
flowchart LR
CultureMech_003097["mj_medium_for_defferibacter_sp_strain_nejineji"]
ing0_NaCl["NaCl"]
CultureMech_003097 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003097 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003097 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_KCl["KCl"]
CultureMech_003097 --> ing3_KCl
CHEBI_32588["CHEBI:32588"]
ing3_KCl -.-> CHEBI_32588
ing4_NH4Cl["NH4Cl"]
CultureMech_003097 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_K2HPO4["K2HPO4"]
CultureMech_003097 --> ing5_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing5_K2HPO4 -.-> CHEBI_131527
ing6_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003097 --> ing6_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing6_MnCl2_x_4_H2O -.-> CHEBI_86368
ing7_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003097 --> ing7_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing7_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003097 --> ing8_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003097 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_ZnCl2["ZnCl2"]
CultureMech_003097 --> ing10_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing10_ZnCl2 -.-> CHEBI_49976
ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003097 --> ing11_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003097 --> ing12_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
ing13_Na2WO2_x_2_H2O["Na2WO2 x 2 H2O"]
CultureMech_003097 --> ing13_Na2WO2_x_2_H2O
ing14_H3BO3["H3BO3"]
CultureMech_003097 --> ing14_H3BO3
CHEBI_33118["CHEBI:33118"]
ing14_H3BO3 -.-> CHEBI_33118
ing15_Sulfur["Sulfur"]
CultureMech_003097 --> ing15_Sulfur
CHEBI_26833["CHEBI:26833"]
ing15_Sulfur -.-> CHEBI_26833
ing16_Resazurin["Resazurin"]
CultureMech_003097 --> ing16_Resazurin
CHEBI_8806["CHEBI:8806"]
ing16_Resazurin -.-> CHEBI_8806
ing17_NaHCO3["NaHCO3"]
CultureMech_003097 --> ing17_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing17_NaHCO3 -.-> CHEBI_32139
ing18_Biotin["Biotin"]
CultureMech_003097 --> ing18_Biotin
CHEBI_15956["CHEBI:15956"]
ing18_Biotin -.-> CHEBI_15956
ing19_Folic_acid["Folic acid"]
CultureMech_003097 --> ing19_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing19_Folic_acid -.-> CHEBI_27470
ing20_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003097 --> ing20_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing20_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing21_Thiamine_HCl["Thiamine HCl"]
CultureMech_003097 --> ing21_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing21_Thiamine_HCl -.-> CHEBI_49105
ing22_Riboflavin["Riboflavin"]
CultureMech_003097 --> ing22_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing22_Riboflavin -.-> CHEBI_17015
ing23_Nicotinic_acid["Nicotinic acid"]
CultureMech_003097 --> ing23_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing23_Nicotinic_acid -.-> CHEBI_15940
ing24_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003097 --> ing24_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing24_Calcium_pantothenate -.-> CHEBI_31345
ing25_Vitamin_B12["Vitamin B12"]
CultureMech_003097 --> ing25_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing25_Vitamin_B12 -.-> CHEBI_176843
ing26_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003097 --> ing26_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing26_p_Aminobenzoic_acid -.-> CHEBI_30753
ing27_Lipoic_acid["Lipoic acid"]
CultureMech_003097 --> ing27_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing27_Lipoic_acid -.-> CHEBI_16494
Ingredients (28)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
29.5858 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
6.41026 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.493097 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
KCl
Cl.K 74.551 g/mol
|
0.325444 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.246548 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.138067 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 7 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.00394477 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 8 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.00394477 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 9 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.00197239 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.00197239 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.000493097 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 12 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.000394477 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 13 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.000246548 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 14 | Na2WO2 x 2 H2O | 9.86193e-05 G_PER_L | ||
| 15 |
H3BO3
H3BO3 61.833 g/mol
|
9.86193e-05 G_PER_L |
CHEBI:33118
boric acid
|
|
| 16 |
Sulfur
S 32.067 g/mol
|
9.86193 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 17 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000493097 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 18 |
NaHCO3
CHO3.Na 84.006 g/mol
|
12.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 19 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 20 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 21 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 22 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 23 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 24 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 25 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 26 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 27 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 28 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components except sulfur and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add the following solutions from anaerobic stocks (filter-sterilized):
- Separately steam sulfur for 3 hr on each of 3 successive days.
- To complete the medium, distribute the solution into sterile culture vessels containing appropriate amount of sulfur (e.g., 5 ml solution and 0.05 g sulfur in Hungate tubes) under a H2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Then, add Na2S x 9H2O solution from an anaerobic stock (final 0.1 g/L). After inoculation, pressurize the inoculated culture vessels to 200 kPa H2-CO2 (4:1, v/v).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mj_medium_for_defferibacter_sp_strain_nejineji (CultureMech:003097)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.494227Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J754 |
| 2026-03-13T06:19:17.789165Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003097 |
| 2026-03-17T06:30:24.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.554358+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MJ MEDIUM FOR DEFFERIBACTER SP. STRAIN NEJINEJI" to "mj_medium_for_defferibacter_sp_strain_nejineji" for consistent matching |
| 2026-06-05T07:41:34.973485+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 27 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.133476+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.670460+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fb1ffbb2e0da426de5d9b3f94fdb24b616247fa2e4002320f4996283788bf340 |