Overview
- External ontology
- mediadive.medium:J760 (source link unavailable) — DESULFURISPIRILLUM ALKALIPHILUM MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=760
Composition
flowchart LR
CultureMech_003104["desulfurispirillum_alkaliphilum_medium"]
ing0_Na2CO3["Na2CO3"]
CultureMech_003104 --> ing0_Na2CO3
CHEBI_29377["CHEBI:29377"]
ing0_Na2CO3 -.-> CHEBI_29377
ing1_NaHCO3["NaHCO3"]
CultureMech_003104 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_NaCl["NaCl"]
CultureMech_003104 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_K2HPO4["K2HPO4"]
CultureMech_003104 --> ing3_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing3_K2HPO4 -.-> CHEBI_131527
ing4_MgSO4["MgSO4"]
CultureMech_003104 --> ing4_MgSO4
CHEBI_32599["CHEBI:32599"]
ing4_MgSO4 -.-> CHEBI_32599
ing5_Yeast_extract["Yeast extract"]
CultureMech_003104 --> ing5_Yeast_extract
ing6_Sodium_acetate["Sodium acetate"]
CultureMech_003104 --> ing6_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate -.-> CHEBI_32954
ing7_KNO3["KNO3"]
CultureMech_003104 --> ing7_KNO3
CHEBI_63043["CHEBI:63043"]
ing7_KNO3 -.-> CHEBI_63043
ing8_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003104 --> ing8_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing8_Na2S_x_9_H2O -.-> CHEBI_76209
ing9_EDTA["EDTA"]
CultureMech_003104 --> ing9_EDTA
CHEBI_4735["CHEBI:4735"]
ing9_EDTA -.-> CHEBI_4735
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003104 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003104 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003104 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_H3BO3["H3BO3"]
CultureMech_003104 --> ing13_H3BO3
CHEBI_33118["CHEBI:33118"]
ing13_H3BO3 -.-> CHEBI_33118
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003104 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003104 --> ing15_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing15_CuCl2_x_2_H2O -.-> CHEBI_86318
ing16_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003104 --> ing16_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing16_NiCl2_x_6_H2O -.-> CHEBI_34887
ing17_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003104 --> ing17_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing17_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (18)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Na2CO3
CO3.2Na 105.988 g/mol
|
21.0325 G_PER_L |
CHEBI:29377
sodium carbonate
|
|
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
7.64818 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
5.73614 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.478011 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 5 |
MgSO4
Mg.O4S 120.368 g/mol
|
10 G_PER_L |
CHEBI:32599
magnesium sulfate
|
|
| 6 | Yeast extract | 2 G_PER_L | ||
| 7 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
20 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
KNO3
K.NO3 101.102 g/mol
|
10 G_PER_L |
CHEBI:63043
potassium nitrate
|
|
| 9 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
2.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 10 |
EDTA
C10H16N2O8 292.244 g/mol
|
0.5 G_PER_L |
CHEBI:4735
ethylenediaminetetraacetic acid
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.2 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 17 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 18 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components and autoclave under a N2 atmosphere. Then add the following solutions from sterile anaerobic stocks:
- Mix well until clarified. Finally, add the following solution to the medium:
- Comment: Incubate with a 5-10% inoculum. The strain also grows in the medium with sulfur as an electron acceptor. In this case, use the above described medium, replacing KNO3 with 0.25 g/L NH4Cl and 2.0 g/L sulfur (separately autoclaved at 110C for 40 min).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfurispirillum_alkaliphilum_medium (CultureMech:003104)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.519872Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J760 |
| 2026-03-13T06:19:17.854822Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003104 |
| 2026-03-17T06:30:00.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.629302+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFURISPIRILLUM ALKALIPHILUM MEDIUM" to "desulfurispirillum_alkaliphilum_medium" for consistent matching |
| 2026-06-05T07:40:58.212937+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:34.713617+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:46:56.194005+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:32.572661+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 0354969c8e21ec1cc591d22a33077c20c8eaee3f1fba5828fc9b676a2f4b515f |