Overview
- External ontology
- mediadive.medium:J761 (source link unavailable) — MYCOPLASMA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=761
Composition
flowchart LR
CultureMech_003105["mycoplasma_medium"]
ing0_Heart_Infusion_Broth["Heart Infusion Broth"]
CultureMech_003105 --> ing0_Heart_Infusion_Broth
ing1_Agar["Agar"]
CultureMech_003105 --> ing1_Agar
CHEBI_2509["CHEBI:2509"]
ing1_Agar -.-> CHEBI_2509
ing2_Horse_serum["Horse serum"]
CultureMech_003105 --> ing2_Horse_serum
ing3_Yeast_extract["Yeast extract"]
CultureMech_003105 --> ing3_Yeast_extract
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Heart Infusion Broth | 17.5 G_PER_L | ||
| 2 | Agar | 10 G_PER_L |
CHEBI:2509
agar
|
|
| 3 | Horse serum | 200 G_PER_L | ||
| 4 | Yeast extract | 100 G_PER_L |
Preparation
- Autoclave at 121C for 15 min. Cool to 55C and aseptically add the following filter-sterilized solutions:
- Warm serum and yeast extract solution to 55C before adding to the basal medium. Adjust pH to 7.4&;0.2 (final).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mycoplasma_medium (CultureMech:003105)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.523395Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J761 |
| 2026-03-13T06:19:17.859611Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003105 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.635248+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MYCOPLASMA MEDIUM" to "mycoplasma_medium" for consistent matching |
| 2026-06-05T07:41:39.031225+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 1 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:50.909621+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b532b60d389e076298eee5d9bd6405758af729895cf63653b011a3344960e32c |