Overview
- External ontology
- mediadive.medium:J769 (source link unavailable) — MODIFIED R2A MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=769
Composition
flowchart LR
CultureMech_003110["modified_r2a_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003110 --> ing0_Yeast_extract
ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
CultureMech_003110 --> ing1_Proteose_peptone_no__3
ing2_Casamino_acids["Casamino acids"]
CultureMech_003110 --> ing2_Casamino_acids
ing3_Glucose["Glucose"]
CultureMech_003110 --> ing3_Glucose
CHEBI_17234["CHEBI:17234"]
ing3_Glucose -.-> CHEBI_17234
ing4_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003110 --> ing4_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing4_Sodium_pyruvate -.-> CHEBI_50144
ing5_Trisodium_citrate["Trisodium citrate"]
CultureMech_003110 --> ing5_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing5_Trisodium_citrate -.-> CHEBI_53258
ing6_K2HPO4["K2HPO4"]
CultureMech_003110 --> ing6_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing6_K2HPO4 -.-> CHEBI_131527
ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003110 --> ing7_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
ing8_NaCl["NaCl"]
CultureMech_003110 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
Ingredients (9)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.5 G_PER_L | ||
| 2 | Proteose peptone no. 3 | 0.5 G_PER_L | ||
| 3 | Casamino acids | 0.5 G_PER_L | ||
| 4 |
Glucose
C6H12O6 180.156 g/mol
|
0.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 5 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.5 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 6 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 7 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.3 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 8 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
20 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
230 G_PER_L |
CHEBI:26710
sodium chloride
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2.
- For preparation of solid medium, add 18.0 g/L washed agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_r2a_medium (CultureMech:003110)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.542953Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J769 |
| 2026-01-28T19:48:17.545029Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:17.886347Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003110 |
| 2026-03-17T06:30:02.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.667390+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED R2A MEDIUM" to "modified_r2a_medium" for consistent matching |
| 2026-06-05T07:41:37.663745+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.058482+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:47:08.922513+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:49.697657+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 136e15b35a7f66ad5943d744709cc670d9a0077960f52dee96066ccfa04239f5 |