modified_r2a_medium

CultureMech:003110 bacterial COMPLEX LIQUID
Original name: MODIFIED R2A MEDIUM

Overview

External ontology
mediadive.medium:J769 (source link unavailable) — MODIFIED R2A MEDIUM
Applications
  • Microbial cultivation
pH
7.1
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=769

Composition

flowchart LR
    CultureMech_003110["modified_r2a_medium"]
    ing0_Yeast_extract["Yeast extract"]
    CultureMech_003110 --> ing0_Yeast_extract
    ing1_Proteose_peptone_no__3["Proteose peptone no. 3"]
    CultureMech_003110 --> ing1_Proteose_peptone_no__3
    ing2_Casamino_acids["Casamino acids"]
    CultureMech_003110 --> ing2_Casamino_acids
    ing3_Glucose["Glucose"]
    CultureMech_003110 --> ing3_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing3_Glucose -.-> CHEBI_17234
    ing4_Sodium_pyruvate["Sodium pyruvate"]
    CultureMech_003110 --> ing4_Sodium_pyruvate
    CHEBI_50144["CHEBI:50144"]
    ing4_Sodium_pyruvate -.-> CHEBI_50144
    ing5_Trisodium_citrate["Trisodium citrate"]
    CultureMech_003110 --> ing5_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing5_Trisodium_citrate -.-> CHEBI_53258
    ing6_K2HPO4["K2HPO4"]
    CultureMech_003110 --> ing6_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing6_K2HPO4 -.-> CHEBI_131527
    ing7_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003110 --> ing7_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing7_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing8_NaCl["NaCl"]
    CultureMech_003110 --> ing8_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing8_NaCl -.-> CHEBI_26710

Ingredients (9)

# Ingredient Concentration Ontology Notes
1 Yeast extract 0.5 G_PER_L
2 Proteose peptone no. 3 0.5 G_PER_L
3 Casamino acids 0.5 G_PER_L
4 Glucose
C6H12O6 180.156 g/mol
0.5 G_PER_L CHEBI:17234
glucose
5 Sodium pyruvate
C3H3O3.Na 110.044 g/mol
0.5 G_PER_L CHEBI:50144
sodium pyruvate
6 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
3 G_PER_L CHEBI:53258
sodium citrate
7 K2HPO4
HO4P.2K 174.174 g/mol
0.3 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
8 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
20 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
9 NaCl
Cl.Na 58.443 g/mol
230 G_PER_L CHEBI:26710
sodium chloride

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2.
  2. For preparation of solid medium, add 18.0 g/L washed agar.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.542953Z mediadive-import Imported from MediaDive Source: JCM, ID: J769
2026-01-28T19:48:17.545029Z chebi-enrichment Added CHEBI ontology terms to ingredients Enriched using MicrobeMediaParam and MediaDive chemical mappings
2026-03-13T06:19:17.886347Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003110
2026-03-17T06:30:02.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.667390+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MODIFIED R2A MEDIUM" to "modified_r2a_medium" for consistent matching
2026-06-05T07:41:37.663745+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-08T23:19:49.058482+00:00 chebi-regrounding-v1.0 Corrected CHEBI ingredient grounding (audit G21/G22) Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI).
2026-06-10T07:47:08.922513+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:49.697657+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 136e15b35a7f66ad5943d744709cc670d9a0077960f52dee96066ccfa04239f5