caldinitratiruptor_microaerophilus_medium

CultureMech:003125 bacterial COMPLEX LIQUID
Original name: CALDINITRATIRUPTOR MICROAEROPHILUS MEDIUM

Overview

External ontology
mediadive.medium:J782 (source link unavailable) — CALDINITRATIRUPTOR MICROAEROPHILUS MEDIUM
Applications
  • Microbial cultivation
pH
7.2
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=782

Composition

flowchart LR
    CultureMech_003125["caldinitratiruptor_microaerophilus_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003125 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003125 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003125 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_KNO3["KNO3"]
    CultureMech_003125 --> ing3_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing3_KNO3 -.-> CHEBI_63043
    ing4_NaCl["NaCl"]
    CultureMech_003125 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_KCl["KCl"]
    CultureMech_003125 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003125 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003125 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_003125 --> ing8_Yeast_extract
    ing9_NaHCO3["NaHCO3"]
    CultureMech_003125 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_Glucose["Glucose"]
    CultureMech_003125 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003125 --> ing11_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing11_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing12_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003125 --> ing12_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing12_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003125 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003125 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003125 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003125 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003125 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003125 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_003125 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003125 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (21)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.28436 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.28436 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.947867 G_PER_L CHEBI:31206
ammonium chloride
4 KNO3
K.NO3 101.102 g/mol
1.61137 G_PER_L CHEBI:63043
potassium nitrate
5 NaCl
Cl.Na 58.443 g/mol
1.947867 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 0.947867, 1.0]
6 KCl
Cl.K 74.551 g/mol
0.0947867 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1947867 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0947867, 0.1]
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
0.236967 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 Yeast extract 0.947867 G_PER_L
10 NaHCO3
CHO3.Na 84.006 g/mol
25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
12 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
13 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
14 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly, adjust pH to 7.2 with KOH and distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.584009Z mediadive-import Imported from MediaDive Source: JCM, ID: J782
2026-03-13T03:09:43.203341Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:17.997086Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003125
2026-03-17T06:30:55.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:31.793946+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "CALDINITRATIRUPTOR MICROAEROPHILUS MEDIUM" to "caldinitratiruptor_microaerophilus_medium" for consistent matching
2026-06-05T07:40:52.205299+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 18 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.608051+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:54.189618+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:26.495953+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a882ad30b50962c0bb119ec354a8e277c9e86065d6671d799d365da2d59ac1c8