Overview
- External ontology
- mediadive.medium:J783 (source link unavailable) — CLOSTRIDIUM TH7C1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=783
Composition
flowchart LR
CultureMech_003126["clostridium_th7c1_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003126 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_003126 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_003126 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_003126 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_003126 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003126 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003126 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Yeast_extract["Yeast extract"]
CultureMech_003126 --> ing7_Yeast_extract
ing8_Trypticase_peptone["Trypticase peptone"]
CultureMech_003126 --> ing8_Trypticase_peptone
ing9_Sodium_acetate["Sodium acetate"]
CultureMech_003126 --> ing9_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing9_Sodium_acetate -.-> CHEBI_32954
ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003126 --> ing10_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing11_Resazurin["Resazurin"]
CultureMech_003126 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_NaHCO3["NaHCO3"]
CultureMech_003126 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Glucose["Glucose"]
CultureMech_003126 --> ing13_Glucose
CHEBI_17234["CHEBI:17234"]
ing13_Glucose -.-> CHEBI_17234
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003126 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003126 --> ing15_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing15_Nitrilotriacetic_acid -.-> CHEBI_44557
ing16_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003126 --> ing16_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing16_MgSO4_x_7_H2O -.-> CHEBI_31795
ing17_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003126 --> ing17_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing17_MnSO4_x_n_H2O -.-> CHEBI_86360
ing18_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003126 --> ing18_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing18_FeSO4_x_7_H2O -.-> CHEBI_75836
ing19_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003126 --> ing19_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing19_CoSO4_x_7_H2O -.-> CHEBI_91244
ing20_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003126 --> ing20_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing20_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing21_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003126 --> ing21_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing21_CuSO4_x_5_H2O -.-> CHEBI_31440
ing22_AlK_SO4_2["AlK(SO4)2"]
CultureMech_003126 --> ing22_AlK_SO4_2
CHEBI_86463["CHEBI:86463"]
ing22_AlK_SO4_2 -.-> CHEBI_86463
ing23_H3BO3["H3BO3"]
CultureMech_003126 --> ing23_H3BO3
CHEBI_33118["CHEBI:33118"]
ing23_H3BO3 -.-> CHEBI_33118
ing24_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003126 --> ing24_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing24_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (25)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28222 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.28222 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.940734 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1.470367 G_PER_L |
CHEBI:26710
sodium chloride
|
[Merged 2 duplicates: 0.470367, 1.0] |
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.0940734 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1940734 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
[Merged 2 duplicates: 0.0940734, 0.1] |
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.470367 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 | Yeast extract | 0.940734 G_PER_L | ||
| 9 | Trypticase peptone | 0.940734 G_PER_L | ||
| 10 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.150517 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 11 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.470367 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000940734 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
Glucose
C6H12O6 180.156 g/mol
|
20 G_PER_L |
CHEBI:17234
glucose
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
8 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
1.5 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 17 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
3 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 18 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.5 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 19 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 20 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
0.1 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
|
| 21 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 22 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 23 |
AlK(SO4)2
Al.K.2O4S 258.206 g/mol
|
0.01 G_PER_L |
CHEBI:86463
potassium aluminium sulfate
|
|
| 24 |
H3BO3
H3BO3 61.833 g/mol
|
0.01 G_PER_L |
CHEBI:33118
boric acid
|
|
| 25 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.01 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components thoroughly, adjust pH to 7.3 with KOH, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
- Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- clostridium_th7c1_medium (CultureMech:003126)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.588236Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J783 |
| 2026-03-13T03:08:58.819839Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 2 duplicate ingredient(s) |
| 2026-03-13T06:19:18.008082Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003126 |
| 2026-03-17T06:30:03.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.806100+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "CLOSTRIDIUM TH7C1 MEDIUM" to "clostridium_th7c1_medium" for consistent matching |
| 2026-06-05T07:40:54.280878+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:54.830904+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:28.595722+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 011dd2c5789dffecb45767e41e6dc6cbc9388bfbee81c6ccf0cc5d9ea24d2564 |