Overview
- External ontology
- mediadive.medium:J788 (source link unavailable) — NATRONOARCHAEUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 8.7
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=788
Composition
flowchart LR
CultureMech_003131["natronoarchaeum_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_003131 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_003131 --> ing1_Yeast_extract
ing2_Glucose["Glucose"]
CultureMech_003131 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Na_glutamate["Na glutamate"]
CultureMech_003131 --> ing3_Na_glutamate
CHEBI_64220["CHEBI:64220"]
ing3_Na_glutamate -.-> CHEBI_64220
ing4_Trisodium_citrate["Trisodium citrate"]
CultureMech_003131 --> ing4_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing4_Trisodium_citrate -.-> CHEBI_53258
ing5_K2HSO4["K2HSO4"]
CultureMech_003131 --> ing5_K2HSO4
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003131 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_KCl["KCl"]
CultureMech_003131 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_NaCl["NaCl"]
CultureMech_003131 --> ing8_NaCl
CHEBI_26710["CHEBI:26710"]
ing8_NaCl -.-> CHEBI_26710
ing9_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003131 --> ing9_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing9_FeCl2_x_4_H2O -.-> CHEBI_86249
ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003131 --> ing10_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 4 G_PER_L | ||
| 2 | Yeast extract | 0.5 G_PER_L | ||
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
3 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Na glutamate
C5H8NO4.Na 169.112 g/mol
|
0.1 G_PER_L |
CHEBI:64220
monosodium glutamate
|
|
| 5 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
0.3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 6 | K2HSO4 | 1 G_PER_L | ||
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
2 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
NaCl
Cl.Na 58.443 g/mol
|
220 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 10 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
36 MG_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 11 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.36 MG_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 8.5 - 8.8 before autoclaving.
- For preparation of solid medium, add 20.0 g/L Agar, Noble (BD-Difco).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- natronoarchaeum_medium (CultureMech:003131)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.609287Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J788 |
| 2026-01-28T19:48:20.110961Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.056453Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003131 |
| 2026-03-17T06:30:08.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.860969+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NATRONOARCHAEUM MEDIUM" to "natronoarchaeum_medium" for consistent matching |
| 2026-06-05T07:41:39.360450+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 9 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.340499+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:04:32.057963+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 27e57f4481996ff159ec71c30846bb82a81a73b7e967423983b1a16a8b71095f |