Overview
- External ontology
- mediadive.medium:J793 (source link unavailable) — NHA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=793
Composition
flowchart LR
CultureMech_003137["nha_medium"]
ing0_NaCl["NaCl"]
CultureMech_003137 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003137 --> ing1_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
ing2_KCl["KCl"]
CultureMech_003137 --> ing2_KCl
CHEBI_32588["CHEBI:32588"]
ing2_KCl -.-> CHEBI_32588
ing3_Trisodium_citrate["Trisodium citrate"]
CultureMech_003137 --> ing3_Trisodium_citrate
CHEBI_53258["CHEBI:53258"]
ing3_Trisodium_citrate -.-> CHEBI_53258
ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003137 --> ing4_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
ing5_Casamino_acids["Casamino acids"]
CultureMech_003137 --> ing5_Casamino_acids
Ingredients (6)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
10 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 3 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 4 |
Trisodium citrate
C6H5O7.3Na 258.069 g/mol
|
3 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 5 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.2 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 6 | Casamino acids | 5 G_PER_L |
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.0 - 7.2.
- For preparation of solid medium, add 20.0 g/L agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- nha_medium (CultureMech:003137)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.620707Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J793 |
| 2026-01-28T19:48:10.935730Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.085319Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003137 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.893054+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NHA MEDIUM" to "nha_medium" for consistent matching |
| 2026-06-05T07:41:39.651030+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:49.390055+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:05:51.321055+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint b6eea1a73b0f9627032cf0d196f7bfc52ad3c1700bf3336ba16cf09b70358fb7 |