Overview
- External ontology
- mediadive.medium:J79 (source link unavailable) — METHANOL-UTILIZING BACTERIA MEDIUM B
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=79
Composition
flowchart LR
CultureMech_003144["methanol_utilizing_bacteria_medium_b"]
ing0_Methanol["Methanol"]
CultureMech_003144 --> ing0_Methanol
CHEBI_17790["CHEBI:17790"]
ing0_Methanol -.-> CHEBI_17790
ing1__NH4_2SO4["(NH4)2SO4"]
CultureMech_003144 --> ing1__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing1__NH4_2SO4 -.-> CHEBI_62946
ing2_KH2PO4["KH2PO4"]
CultureMech_003144 --> ing2_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing2_KH2PO4 -.-> CHEBI_63036
ing3_Na2HPO4["Na2HPO4"]
CultureMech_003144 --> ing3_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing3_Na2HPO4 -.-> CHEBI_34683
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003144 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_Ferric_citrate["Ferric citrate"]
CultureMech_003144 --> ing5_Ferric_citrate
CHEBI_144421["CHEBI:144421"]
ing5_Ferric_citrate -.-> CHEBI_144421
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003144 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003144 --> ing7_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing7_MnCl2_x_4_H2O -.-> CHEBI_86368
ing8_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003144 --> ing8_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing8_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003144 --> ing9_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_003144 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Methanol
CH4O 32.042 g/mol
|
10 G_PER_L |
CHEBI:17790
methanol
|
|
| 2 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
2.9703 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 3 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.38614 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 4 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
2.9703 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.19802 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
Ferric citrate
C6H5FeO7 244.944 g/mol
|
0.029703 G_PER_L |
CHEBI:144421
iron(III) citrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.029703 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.00495049 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 9 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00495049 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 10 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.00049505 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.00039604 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
Preparation
- Thiamine x HCl can be replaced by 0.2 g of yeast extract.
- Adjust pH to 7.1.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanol_utilizing_bacteria_medium_b (CultureMech:003144)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.692589Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J79 |
| 2026-03-13T06:19:18.127781Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003144 |
| 2026-03-17T06:30:22.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.940324+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOL-UTILIZING BACTERIA MEDIUM B" to "methanol_utilizing_bacteria_medium_b" for consistent matching |
| 2026-06-05T07:41:31.761481+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.069834+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:44.564867+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint af1aff6362f2ce20ff55425c0f6f22c1389917053e59667ed3207c7eea6b5ecf |