Overview
- External ontology
- mediadive.medium:J801 (source link unavailable) — BHI-S MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=801 Commercial Product: Brain Heart Infusion Supplier: Difco/BD Catalog: 237500 Source: https://microbenotes.com/brain-heart-infusion-bhi-agar/ Accessed: 2026-03-28 Constituent composition researched from product specification.
Composition
flowchart LR
CultureMech_003146["bhi_s_medium"]
ing0_Calf_brains["Calf brains"]
CultureMech_003146 --> ing0_Calf_brains
ing1_Beef_heart["Beef heart"]
CultureMech_003146 --> ing1_Beef_heart
ing2_Proteose_peptone["Proteose peptone"]
CultureMech_003146 --> ing2_Proteose_peptone
ing3_Dextrose["Dextrose"]
CultureMech_003146 --> ing3_Dextrose
CHEBI_17634["CHEBI:17634"]
ing3_Dextrose -.-> CHEBI_17634
ing4_Sodium_chloride["Sodium chloride"]
CultureMech_003146 --> ing4_Sodium_chloride
CHEBI_26710["CHEBI:26710"]
ing4_Sodium_chloride -.-> CHEBI_26710
ing5_Disodium_phosphate["Disodium phosphate"]
CultureMech_003146 --> ing5_Disodium_phosphate
CHEBI_34683["CHEBI:34683"]
ing5_Disodium_phosphate -.-> CHEBI_34683
ing6_NaCl["NaCl"]
CultureMech_003146 --> ing6_NaCl
ing6_NaCl -.-> CHEBI_26710
ing7_PIPES["PIPES"]
CultureMech_003146 --> ing7_PIPES
CHEBI_39033["CHEBI:39033"]
ing7_PIPES -.-> CHEBI_39033
ing8_Sulfur["Sulfur"]
CultureMech_003146 --> ing8_Sulfur
CHEBI_26833["CHEBI:26833"]
ing8_Sulfur -.-> CHEBI_26833
ing9_Resazurin["Resazurin"]
CultureMech_003146 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003146 --> ing10_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing10_Na2S_x_9_H2O -.-> CHEBI_76209
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Calf brains
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000955
brain
|
infusion from 200 g/L; Tissue infusion - source material is calf brain tissue; Constituent of Brain Heart Infusion commercial product |
| 2 |
Beef heart
Difco/BD Brain Heart Infusion 237500
|
variable VARIABLE |
UBERON:0000948
heart
|
infusion from 250 g/L; Tissue infusion - source material is beef/ox heart tissue; Constituent of Brain Heart Infusion commercial product |
| 3 |
Proteose peptone
Difco/BD Brain Heart Infusion 237500
|
10.0 G_PER_L |
MICRO:0000180 (source link unavailable)
proteose peptone
|
Enzymatic digest of animal protein providing amino acids and peptides; Constituent of Brain Heart Infusion commercial product |
| 4 |
Dextrose
C6H12O6 180.156 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.0 G_PER_L |
CHEBI:17634
D-glucose
|
Carbon/energy source; Constituent of Brain Heart Infusion commercial product |
| 5 |
Sodium chloride
Cl.Na 58.443 g/mol
Difco/BD Brain Heart Infusion 237500
|
5.0 G_PER_L |
CHEBI:26710
sodium chloride
|
Maintains osmotic equilibrium; Constituent of Brain Heart Infusion commercial product |
| 6 |
Disodium phosphate
HO4P.2Na 141.958 g/mol
Difco/BD Brain Heart Infusion 237500
|
2.5 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
pH buffer (final pH 7.4 ± 0.2); Constituent of Brain Heart Infusion commercial product |
| 7 |
NaCl
Cl.Na 58.443 g/mol
|
23 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 8 | PIPES | 6.05 G_PER_L |
CHEBI:39033
PIPES
|
|
| 9 |
Sulfur
S 32.067 g/mol
|
10 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.001 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
Preparation
- Mix ingredients except sulfur and Na2S x 9H2O, adjust pH to 7.0 - 7.2 and autoclave under N2 atmosphere. Steam sulfur for 3 hr on each of 3 successive days. Neutralize Na2S x 9H2O as a 5% solution and autoclave under a N2 gas atmosphere. Distribute the medium and sulfur into culture vessels under a N2 stream, and seal with butyl rubber stoppers. Prior to inoculation, add the sterile Na2S x 9H2O solution and readjust pH to 7.0 - 7.2, if necessary.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- bhi_s_medium (CultureMech:003146)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.642411Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J801 |
| 2026-03-13T06:19:18.134940Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003146 |
| 2026-03-17T06:30:49.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:31.948585+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "BHI-S MEDIUM" to "bhi_s_medium" for consistent matching |
| 2026-06-05T07:40:50.927543+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:25.163001+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint e71412b83750b90305cc02d5d94742dfbb54bc4f808161fcda2d65f5fe240b3e |