thermococcales_rich_medium

Overview

External ontology

mediadive.medium:J811 (source link unavailable) — THERMOCOCCALES RICH MEDIUM

Applications

• Microbial cultivation

pH

6.8

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=811

Composition

flowchart LR
    CultureMech_003156["thermococcales_rich_medium"]
    ing0_PIPES["PIPES"]
    CultureMech_003156 --> ing0_PIPES
    CHEBI_39033["CHEBI:39033"]
    ing0_PIPES -.-> CHEBI_39033
    ing1_NaCl["NaCl"]
    CultureMech_003156 --> ing1_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing1_NaCl -.-> CHEBI_26710
    ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003156 --> ing2_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing3_KCl["KCl"]
    CultureMech_003156 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4__NH4_2SO4["(NH4)2SO4"]
    CultureMech_003156 --> ing4__NH4_2SO4
    CHEBI_62946["CHEBI:62946"]
    ing4__NH4_2SO4 -.-> CHEBI_62946
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003156 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_KH2PO4["KH2PO4"]
    CultureMech_003156 --> ing6_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing6_KH2PO4 -.-> CHEBI_63036
    ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003156 --> ing7_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing8_NaBr["NaBr"]
    CultureMech_003156 --> ing8_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing8_NaBr -.-> CHEBI_63004
    ing9_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_003156 --> ing9_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing9_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing10_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003156 --> ing10_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing10_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing11_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003156 --> ing11_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing11_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing12_Yeast_extract["Yeast extract"]
    CultureMech_003156 --> ing12_Yeast_extract
    ing13_Tryptone["Tryptone"]
    CultureMech_003156 --> ing13_Tryptone
    ing14_Sulfur["Sulfur"]
    CultureMech_003156 --> ing14_Sulfur
    CHEBI_26833["CHEBI:26833"]
    ing14_Sulfur -.-> CHEBI_26833
    ing15_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003156 --> ing15_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing15_Na2S_x_9_H2O -.-> CHEBI_76209
    ing16_Resazurin["Resazurin"]
    CultureMech_003156 --> ing16_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing16_Resazurin -.-> CHEBI_8806

Ingredients (17)

Preparation

1. Mix ingredients except Na2S x 9H2O, adjust pH to 6.8 and distribute the medium into culture vessels (e.g., 70 ml in 120 ml serum bottles). Bubble the medium with N2 gas for 30 min and seal with butyl rubber stoppers. Steam the medium for 3 hr on each of 3 successive days. Reduce the medium with sterile Na2S x 9H2O solution (neutralized and autoclaved under a N2 atmosphere) and readjust pH to 6.8, if necessary.
2. Comment: For cultivation of JCM 16557, inoculated cultures should be pressurized to 20 to 100 MPa. Therefore, aspirate the culture into a plastic syringe with a 18G needle and stick the needle into a silicone rubber stopper. Put the assemblage of the syringe, needle and rubber stopper in a pressure vessel, pour water into the vessel and keep pressure inside between 20 and 100 MPa (at 90C).

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• thermococcales_rich_medium (CultureMech:003156)