acidic_diluted_asm_medium

CultureMech:003159 bacterial DEFINED LIQUID
Original name: ACIDIC DILUTED ASM MEDIUM

Overview

External ontology
mediadive.medium:J815 (source link unavailable) — ACIDIC DILUTED ASM MEDIUM
Applications
  • Microbial cultivation
pH
3.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=815

Composition

flowchart LR
    CultureMech_003159["acidic_diluted_asm_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003159 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003159 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003159 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003159 --> ing3_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing3_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing4_Na2_EDTA["Na2-EDTA"]
    CultureMech_003159 --> ing4_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing4_Na2_EDTA -.-> CHEBI_64734
    ing5_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003159 --> ing5_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing5_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing6_H3BO3["H3BO3"]
    CultureMech_003159 --> ing6_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing6_H3BO3 -.-> CHEBI_33118
    ing7_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003159 --> ing7_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing7_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing8_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003159 --> ing8_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing8_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing9_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003159 --> ing9_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing9_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003159 --> ing10_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing11_Fe_III__EDTA["Fe(III)-EDTA"]
    CultureMech_003159 --> ing11_Fe_III__EDTA
    CHEBI_30729["CHEBI:30729"]
    ing11_Fe_III__EDTA -.-> CHEBI_30729
    ing12_KH2PO4["KH2PO4"]
    CultureMech_003159 --> ing12_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing12_KH2PO4 -.-> CHEBI_63036
    ing13_Na2HPO4_x_2_H2O["Na2HPO4 x 2 H2O"]
    CultureMech_003159 --> ing13_Na2HPO4_x_2_H2O
    CHEBI_91258["CHEBI:91258"]
    ing13_Na2HPO4_x_2_H2O -.-> CHEBI_91258

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.0998004 G_PER_L CHEBI:31206
ammonium chloride
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0998004 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0199601 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.4 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
5 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.25 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
6 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.02 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
7 H3BO3
H3BO3 61.833 g/mol
0.015 G_PER_L CHEBI:33118
boric acid
8 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.04 G_PER_L CHEBI:75213
sodium molybdate dihydrate
9 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.01 G_PER_L CHEBI:34887
nickel dichloride
10 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
11 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.05 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
12 Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
4.5 G_PER_L CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
13 KH2PO4
H2O4P.K 136.084 g/mol
37.425 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
14 Na2HPO4 x 2 H2O
2H2O.HO4P.2Na 177.988 g/mol
48.95 G_PER_L CHEBI:91258
disodium hydrogenphosphate dihydrate

Preparation

  1. Mix components and autoclave. After cooling, add the following solution (autoclaved) to the medium:
  2. To complete medium, adjust pH to 3.0 with sterile 1 N HCl solution. Then, distribute 20 ml-aliquots of the medium into sterile 120 ml-serum bottles and seal with butyl rubber stoppers. Add 100 ml methane gas (filter-sterilized) into the head space of the culture bottle through a syringe.

Solutions

Phosphate buffer stock solution

Dose: 2 G_PER_L

See mediadive_4768_Phosphate_buffer_stock_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.677257Z mediadive-import Imported from MediaDive Source: JCM, ID: J815
2026-03-09T08:04:16.371444+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:18.214974Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003159
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:29:46.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.042037+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ACIDIC DILUTED ASM MEDIUM" to "acidic_diluted_asm_medium" for consistent matching
2026-05-16T09:03:23.131159+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:47.109414+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.523262+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:21.705455+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 1cac1e6e983b1b25b1082c67755d70ffbaacad569cee321e95ccaf567903f950