methanotroph_1a_medium

CultureMech:003165 bacterial DEFINED LIQUID
Original name: METHANOTROPH 1A MEDIUM

Overview

External ontology
mediadive.medium:J820 (source link unavailable) — METHANOTROPH 1A MEDIUM
Applications
  • Microbial cultivation
pH
6.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=820

Composition

flowchart LR
    CultureMech_003165["methanotroph_1a_medium"]
    ing0_NaNO3["NaNO3"]
    CultureMech_003165 --> ing0_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing0_NaNO3 -.-> CHEBI_63005
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003165 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_Na2HPO4_x_12_H2O["Na2HPO4 x 12 H2O"]
    CultureMech_003165 --> ing2_Na2HPO4_x_12_H2O
    CHEBI_34683["CHEBI:34683"]
    ing2_Na2HPO4_x_12_H2O -.-> CHEBI_34683
    ing3_KH2PO4["KH2PO4"]
    CultureMech_003165 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003165 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003165 --> ing5_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing5_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing6_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003165 --> ing6_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing6_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003165 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003165 --> ing8_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing8_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003165 --> ing9_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing10_H3BO3["H3BO3"]
    CultureMech_003165 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003165 --> ing11_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing11_CoCl2_x_6_H2O -.-> CHEBI_53503

Ingredients (12)

# Ingredient Concentration Ontology Notes
1 NaNO3
NO3.Na 84.994 g/mol
0.999001 G_PER_L CHEBI:63005
sodium nitrate
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.0999001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
3 Na2HPO4 x 12 H2O
HO4P.2Na 141.958 g/mol
0.3996 G_PER_L CHEBI:34683
disodium hydrogenphosphate
4 KH2PO4
H2O4P.K 136.084 g/mol
0.21978 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.02997 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.001998 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
7 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.44 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.2 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.17 G_PER_L CHEBI:86360
manganese(II) sulfate
10 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.06 G_PER_L CHEBI:75213
sodium molybdate dihydrate
11 H3BO3
H3BO3 61.833 g/mol
0.1 G_PER_L CHEBI:33118
boric acid
12 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.08 G_PER_L CHEBI:53503
cobalt chloride hexahydrate

Preparation

  1. Adjust pH to 6.5.
  2. Distribute the medium into culture vessels (leave >80% volume gas phase), seal with butyl rubber stoppers and autoclave. After cooling, add filter-sterilized methane gas to make a final concentration about 20% (v/v) methane in the gas phase.

Solutions

Trace metal solution

Dose: 1 G_PER_L

See mediadive_6140_Trace_metal_solution.yaml for composition

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.694123Z mediadive-import Imported from MediaDive Source: JCM, ID: J820
2026-03-09T08:04:47.121277+00:00 solution-migrator-v1.0 Migrated solutions from ingredients to solutions array Moved 1 solution(s) from ingredients
2026-03-13T06:19:18.257644Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003165
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default solution name
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.089935+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "METHANOTROPH 1A MEDIUM" to "methanotroph_1a_medium" for consistent matching
2026-05-16T09:04:22.921560+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:41:32.971012+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:07.500210+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:44.588012+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 3b0a48848aeb14e9508fdecdf510c36bbdafecb26ad5694853c684f3b3c5b8ad