desulfohalobium_medium

CultureMech:003168 bacterial COMPLEX LIQUID
Original name: DESULFOHALOBIUM MEDIUM

Overview

External ontology
mediadive.medium:J824 (source link unavailable) — DESULFOHALOBIUM MEDIUM
Applications
  • Microbial cultivation
pH
7.0
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=824

Composition

flowchart LR
    CultureMech_003168["desulfohalobium_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003168 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003168 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_003168 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_NaCl["NaCl"]
    CultureMech_003168 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_003168 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_Na2SO4["Na2SO4"]
    CultureMech_003168 --> ing5_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing5_Na2SO4 -.-> CHEBI_32149
    ing6_Sodium_acetate["Sodium acetate"]
    CultureMech_003168 --> ing6_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate -.-> CHEBI_32954
    ing7_Sodium_lactate["Sodium lactate"]
    CultureMech_003168 --> ing7_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing7_Sodium_lactate -.-> CHEBI_75228
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_003168 --> ing8_Yeast_extract
    ing9_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003168 --> ing9_Trypticase_peptone
    ing10_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003168 --> ing10_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing10_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing11_Resazurin["Resazurin"]
    CultureMech_003168 --> ing11_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing11_Resazurin -.-> CHEBI_8806
    ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003168 --> ing12_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing13_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003168 --> ing13_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing13_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003168 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_HCl["HCl"]
    CultureMech_003168 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883
    ing16_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003168 --> ing16_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing16_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing17_ZnCl2["ZnCl2"]
    CultureMech_003168 --> ing17_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing17_ZnCl2 -.-> CHEBI_49976
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003168 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_H3BO3["H3BO3"]
    CultureMech_003168 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003168 --> ing20_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing20_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003168 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003168 --> ing22_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing22_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003168 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
1.10865 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.332594 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.332594 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 NaCl
Cl.Na 58.443 g/mol
110.865 G_PER_L CHEBI:26710
sodium chloride
5 KCl
Cl.K 74.551 g/mol
4.43459 G_PER_L CHEBI:32588
potassium chloride
6 Na2SO4
2Na.O4S 142.043 g/mol
3.32594 G_PER_L CHEBI:32149
sodium sulfate
7 Sodium acetate
C2H3O2.Na 82.034 g/mol
1.10865 G_PER_L CHEBI:32954
sodium acetate
8 Sodium lactate
C3H5O3.Na 112.06 g/mol
1.10865 G_PER_L CHEBI:75228
sodium lactate
9 Yeast extract 1.10865 G_PER_L
10 Trypticase peptone 1.10865 G_PER_L
11 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
3 G_PER_L CHEBI:131361
disodium selenite pentahydrate
12 Resazurin
C12H7NO4 229.191 g/mol
0.00110865 G_PER_L CHEBI:8806
Resazurin
13 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
181.818 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
14 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
24.5455 G_PER_L CHEBI:86158
calcium chloride dihydrate
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
10 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
16 HCl
HCl 36.461 g/mol
2.5 G_PER_L CHEBI:17883
hydrogen chloride
17 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.5 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
18 ZnCl2
2Cl.Zn 136.296 g/mol
0.07 G_PER_L CHEBI:49976
zinc dichloride
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 H3BO3
H3BO3 61.833 g/mol
0.006 G_PER_L CHEBI:33118
boric acid
21 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly and adjust pH to 7.0. Autoclave under a N2 atmosphere.
  2. Autoclave under a N2 atmosphere.
  3. To complete the medium, combine Solutions A and B under a N2 atmosphere. Then, add per liter the following solution from an anaerobic stock:

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.708565Z mediadive-import Imported from MediaDive Source: JCM, ID: J824
2026-03-13T06:19:18.291610Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003168
2026-03-17T06:30:30.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.127660+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOHALOBIUM MEDIUM" to "desulfohalobium_medium" for consistent matching
2026-06-05T07:40:17.741139+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.118508+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:37.297354+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 47f97f345f149283844fcfbbd1c5b02a71665c883500e5d3ced192940fd11eaf