Overview
- External ontology
- mediadive.medium:J829 (source link unavailable) — HALARCHAEUM NEUTROPHILUM MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 5.5
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=829
Composition
flowchart LR
CultureMech_003173["halarchaeum_neutrophilum_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_003173 --> ing0_Casamino_acids
ing1_Tryptone["Tryptone"]
CultureMech_003173 --> ing1_Tryptone
ing2_Yeast_extract["Yeast extract"]
CultureMech_003173 --> ing2_Yeast_extract
ing3_NaCl["NaCl"]
CultureMech_003173 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003173 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_KCl["KCl"]
CultureMech_003173 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Glucose["Glucose"]
CultureMech_003173 --> ing6_Glucose
CHEBI_17234["CHEBI:17234"]
ing6_Glucose -.-> CHEBI_17234
Ingredients (7)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 2 G_PER_L | ||
| 2 | Tryptone | 2 G_PER_L | ||
| 3 | Yeast extract | 2 G_PER_L | ||
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
180 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
20 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
Preparation
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 5.5 with KOH.
- For preparation of solid medium, add 20.0 g/L agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- halarchaeum_neutrophilum_medium (CultureMech:003173)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.730404Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J829 |
| 2026-01-28T19:48:14.081820Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.345802Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003173 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.188871+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "HALARCHAEUM NEUTROPHILUM MEDIUM" to "halarchaeum_neutrophilum_medium" for consistent matching |
| 2026-06-05T07:41:07.118226+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:39.430013+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-08-06T03:04:28.623562+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint cbf02910cca26f2047cd101c0d4f31cf9bc7a3438f36d0aec7c96d41b3ad63e9 |