desulfovibrio_b0109p2_medium

CultureMech:003174 bacterial COMPLEX LIQUID
Original name: DESULFOVIBRIO B0109P2 MEDIUM

Overview

External ontology
mediadive.medium:J830 (source link unavailable) — DESULFOVIBRIO B0109P2 MEDIUM
Applications
  • Microbial cultivation
pH
7.4
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=830

Composition

flowchart LR
    CultureMech_003174["desulfovibrio_b0109p2_medium"]
    ing0_NH4Cl["NH4Cl"]
    CultureMech_003174 --> ing0_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing0_NH4Cl -.-> CHEBI_31206
    ing1_KH2PO4["KH2PO4"]
    CultureMech_003174 --> ing1_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing1_KH2PO4 -.-> CHEBI_63036
    ing2_K2HPO4["K2HPO4"]
    CultureMech_003174 --> ing2_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing2_K2HPO4 -.-> CHEBI_131527
    ing3_Na2SO4["Na2SO4"]
    CultureMech_003174 --> ing3_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing3_Na2SO4 -.-> CHEBI_32149
    ing4_NaCl["NaCl"]
    CultureMech_003174 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_KCl["KCl"]
    CultureMech_003174 --> ing5_KCl
    CHEBI_32588["CHEBI:32588"]
    ing5_KCl -.-> CHEBI_32588
    ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003174 --> ing6_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_003174 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_Yeast_extract["Yeast extract"]
    CultureMech_003174 --> ing8_Yeast_extract
    ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003174 --> ing9_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing10_Resazurin["Resazurin"]
    CultureMech_003174 --> ing10_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing10_Resazurin -.-> CHEBI_8806
    ing11_Sodium_lactate["Sodium lactate"]
    CultureMech_003174 --> ing11_Sodium_lactate
    CHEBI_75228["CHEBI:75228"]
    ing11_Sodium_lactate -.-> CHEBI_75228
    ing12_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003174 --> ing12_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing12_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing13_NaHCO3["NaHCO3"]
    CultureMech_003174 --> ing13_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing13_NaHCO3 -.-> CHEBI_32139
    ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003174 --> ing14_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing14_Na2S_x_9_H2O -.-> CHEBI_76209
    ing15_HCl["HCl"]
    CultureMech_003174 --> ing15_HCl
    CHEBI_17883["CHEBI:17883"]
    ing15_HCl -.-> CHEBI_17883
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003174 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_H3BO3["H3BO3"]
    CultureMech_003174 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003174 --> ing18_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003174 --> ing19_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003174 --> ing20_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003174 --> ing21_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003174 --> ing22_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003174 --> ing23_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (24)

# Ingredient Concentration Ontology Notes
1 NH4Cl
Cl.H4N 53.492 g/mol
0.999001 G_PER_L CHEBI:31206
ammonium chloride
2 KH2PO4
H2O4P.K 136.084 g/mol
0.2997 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
3 K2HPO4
HO4P.2K 174.174 g/mol
0.2997 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
4 Na2SO4
2Na.O4S 142.043 g/mol
2.7972 G_PER_L CHEBI:32149
sodium sulfate
5 NaCl
Cl.Na 58.443 g/mol
19.98 G_PER_L CHEBI:26710
sodium chloride
6 KCl
Cl.K 74.551 g/mol
0.0999001 G_PER_L CHEBI:32588
potassium chloride
7 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0999001 G_PER_L CHEBI:86158
calcium chloride dihydrate
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
3.15684 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 Yeast extract 0.0999001 G_PER_L
10 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.499501 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
11 Resazurin
C12H7NO4 229.191 g/mol
0.000999001 G_PER_L CHEBI:8806
Resazurin
12 Sodium lactate
C3H5O3.Na 112.06 g/mol
20 G_PER_L CHEBI:75228
sodium lactate
13 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
20 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
14 NaHCO3
CHO3.Na 84.006 g/mol
40 G_PER_L CHEBI:32139
sodium hydrogencarbonate
15 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
20 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
16 HCl
HCl 36.461 g/mol
12.5 G_PER_L CHEBI:17883
hydrogen chloride
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
2.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 H3BO3
H3BO3 61.833 g/mol
0.03 G_PER_L CHEBI:33118
boric acid
19 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.1 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
20 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.19 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
21 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.024 G_PER_L CHEBI:34887
nickel dichloride
22 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.002 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
23 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.144 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
24 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.036 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix ingredients and adjust pH to 7.4. Bring to a boil and cool down under a N2-CO2 (4:1, v/v) gas stream. Distribute the medium into culture tubes under the same gas mixture, seal with butyl rubber stoppers and autoclave.
  2. To complete the medium, aseptically and anaerobically add the following stock solutions (per liter, final volume):
  3. Filter-sterilize NaHCO3 solution and store under a N2-CO2 (4:1, v/v) gas mixture. The other solutions are autoclaved and stored anaerobically under a N2 atmosphere.
  4. Readjust pH of the medium to 7.4, if necessary.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.732595Z mediadive-import Imported from MediaDive Source: JCM, ID: J830
2026-03-13T06:19:18.354942Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003174
2026-03-17T06:29:39.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.198847+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DESULFOVIBRIO B0109P2 MEDIUM" to "desulfovibrio_b0109p2_medium" for consistent matching
2026-06-05T07:40:57.728401+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:56.006610+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:32.116983+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint a5c9bbc28b1c9a15ad03e34639135cf8dad5d129217f122730888f8630f4b82d