Overview
- External ontology
- mediadive.medium:J833 (source link unavailable) — IP3-3 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=833
Composition
flowchart LR
CultureMech_003177["ip3_3_medium"]
ing0_NaCl["NaCl"]
CultureMech_003177 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003177 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003177 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NH4Cl["NH4Cl"]
CultureMech_003177 --> ing3_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing3_NH4Cl -.-> CHEBI_31206
ing4_KH2PO4["KH2PO4"]
CultureMech_003177 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_KCl["KCl"]
CultureMech_003177 --> ing5_KCl
CHEBI_32588["CHEBI:32588"]
ing5_KCl -.-> CHEBI_32588
ing6_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
CultureMech_003177 --> ing6_Sodium_acetate_x_3_H2O
CHEBI_32954["CHEBI:32954"]
ing6_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
ing7_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003177 --> ing7_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing7_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing8_Resazurin["Resazurin"]
CultureMech_003177 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_NaHCO3["NaHCO3"]
CultureMech_003177 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Ampicillin["Ampicillin"]
CultureMech_003177 --> ing10_Ampicillin
CHEBI_28971["CHEBI:28971"]
ing10_Ampicillin -.-> CHEBI_28971
ing11_1_1_2_Trichloroethane["1,1,2-Trichloroethane"]
CultureMech_003177 --> ing11_1_1_2_Trichloroethane
CHEBI_36018["CHEBI:36018"]
ing11_1_1_2_Trichloroethane -.-> CHEBI_36018
ing12_Titanium_chloride["Titanium chloride"]
CultureMech_003177 --> ing12_Titanium_chloride
ing13_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003177 --> ing13_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing13_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing14_HCl["HCl"]
CultureMech_003177 --> ing14_HCl
CHEBI_17883["CHEBI:17883"]
ing14_HCl -.-> CHEBI_17883
ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003177 --> ing15_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
ing16_H3BO3["H3BO3"]
CultureMech_003177 --> ing16_H3BO3
CHEBI_33118["CHEBI:33118"]
ing16_H3BO3 -.-> CHEBI_33118
ing17_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003177 --> ing17_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing17_MnCl2_x_4_H2O -.-> CHEBI_86368
ing18_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003177 --> ing18_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing18_CoCl2_x_6_H2O -.-> CHEBI_53503
ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003177 --> ing19_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
ing20_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003177 --> ing20_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing20_CuCl2_x_2_H2O -.-> CHEBI_86318
ing21_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003177 --> ing21_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing21_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing22_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003177 --> ing22_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing22_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing23_NaOH["NaOH"]
CultureMech_003177 --> ing23_NaOH
CHEBI_32145["CHEBI:32145"]
ing23_NaOH -.-> CHEBI_32145
ing24_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
CultureMech_003177 --> ing24_Na2SeO3_x_5_H2O
CHEBI_131361["CHEBI:131361"]
ing24_Na2SeO3_x_5_H2O -.-> CHEBI_131361
ing25_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003177 --> ing25_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing25_Na2WO4_x_2_H2O -.-> CHEBI_63939
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.985222 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.394089 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0985222 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.246305 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.197044 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
KCl
Cl.K 74.551 g/mol
|
0.492611 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 7 |
Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
|
6.69951 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 8 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.246305 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000985222 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
30 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Ampicillin
C16H19N3O4S 349.412 g/mol
|
10 G_PER_L |
CHEBI:28971
ampicillin
|
|
| 12 |
1,1,2-Trichloroethane
C2H3Cl3 133.405 g/mol
|
46 G_PER_L |
CHEBI:36018
1,1,2-trichloroethane
|
|
| 13 | Titanium chloride | 13.6364 G_PER_L | ||
| 14 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
53.4545 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 15 |
HCl
HCl 36.461 g/mol
|
12.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 16 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 17 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 18 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 19 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 20 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 21 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 22 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 23 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 24 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 25 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 26 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components and autoclave under a N2 atmosphere. After cooling, anaerobically add the following sterile solutions (autoclaved or *filter-sterilized) from anaerobic stocks:
- To complete the medium, dispense the medium into culture vessels leaving 40 to 50% volume of gas phase (e.g., 30 ml of the medium in a 50 ml serum bottle) and seal with butyl rubber stoppers. Replace the gas phase with a N2-CO2-H2 (8:1:1, v/v) gas mixture. Finally, add the following reducing solution and chlorosolvent (per liter):
- Adjust pH to 7.0, if necessary.
- Mix components and centrifuge at 8,000 x g for 10 min. Filter-sterilize the supernatant and store under a N2 atmosphere.
Solutions
Vitamin solution (see Medium No. 403)
Dose: 1 G_PER_L
Thiamine solution (see Medium No. 403)
Dose: 1 G_PER_L
Vitamin B12 solution (see Medium No. 403)
Dose: 1 G_PER_L
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- ip3_3_medium (CultureMech:003177)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.743322Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J833 |
| 2026-01-28T19:48:25.231785Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-09T08:04:41.721914+00:00 | solution-migrator-v1.0 | Migrated solutions from ingredients to solutions array | Moved 3 solution(s) from ingredients |
| 2026-03-13T06:19:18.385442Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003177 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default solution name; Added default solution name; Added default solution name |
| 2026-03-17T06:30:18.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.232907+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "IP3-3 MEDIUM" to "ip3_3_medium" for consistent matching |
| 2026-05-16T09:03:37.828355+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:41:09.428266+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 24 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.060286+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:38.466809+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 726cb47b4ab27049508ddfbd44542284245428400f37de1b4c3b605f19ef5168 |