Overview
- External ontology
- mediadive.medium:J844 (source link unavailable) — RSULFAC1 MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=844
Composition
flowchart LR
CultureMech_003189["rsulfac1_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003189 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_003189 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_003189 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_003189 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_KCl["KCl"]
CultureMech_003189 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003189 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003189 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_Yeast_extract["Yeast extract"]
CultureMech_003189 --> ing7_Yeast_extract
ing8_Peptone["Peptone"]
CultureMech_003189 --> ing8_Peptone
ing9_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003189 --> ing9_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing9_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing10_Sulfur["Sulfur"]
CultureMech_003189 --> ing10_Sulfur
CHEBI_26833["CHEBI:26833"]
ing10_Sulfur -.-> CHEBI_26833
ing11_Resazurin["Resazurin"]
CultureMech_003189 --> ing11_Resazurin
CHEBI_8806["CHEBI:8806"]
ing11_Resazurin -.-> CHEBI_8806
ing12_NaHCO3["NaHCO3"]
CultureMech_003189 --> ing12_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing12_NaHCO3 -.-> CHEBI_32139
ing13_Sodium_fumarate["Sodium fumarate"]
CultureMech_003189 --> ing13_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing13_Sodium_fumarate -.-> CHEBI_115156
ing14_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003189 --> ing14_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing14_Na2S_x_9_H2O -.-> CHEBI_76209
ing15_HCl["HCl"]
CultureMech_003189 --> ing15_HCl
CHEBI_17883["CHEBI:17883"]
ing15_HCl -.-> CHEBI_17883
ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003189 --> ing16_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
ing17_H3BO3["H3BO3"]
CultureMech_003189 --> ing17_H3BO3
CHEBI_33118["CHEBI:33118"]
ing17_H3BO3 -.-> CHEBI_33118
ing18_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003189 --> ing18_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing18_MnCl2_x_4_H2O -.-> CHEBI_86368
ing19_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003189 --> ing19_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing19_CoCl2_x_6_H2O -.-> CHEBI_53503
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003189 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003189 --> ing21_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
ing22_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003189 --> ing22_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing22_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing23_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003189 --> ing23_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing23_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (24)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28222 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.28222 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.940734 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
4.70367 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.0940734 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.0940734 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.470367 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 | Yeast extract | 0.940734 G_PER_L | ||
| 9 | Peptone | 1.88147 G_PER_L | ||
| 10 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
0.470367 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 11 |
Sulfur
S 32.067 g/mol
|
9.40734 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 12 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000940734 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 13 |
NaHCO3
CHO3.Na 84.006 g/mol
|
25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 14 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
20 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 15 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
8 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 16 |
HCl
HCl 36.461 g/mol
|
12.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 17 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
2.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 18 |
H3BO3
H3BO3 61.833 g/mol
|
0.03 G_PER_L |
CHEBI:33118
boric acid
|
|
| 19 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 20 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 23 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.144 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 24 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Mix components except sulfur thoroughly and autoclave under a N2-CO2 (4:1, v/v) gas mixture. After cooling, add per liter the following solutions (autoclaved or *filter-sterilized):
- Steam sulfur for 3 hr on each of 3 successive days. Aseptically distribute the medium and sulfur into culture vessels under a stream of N2-CO2 (4:1, v/v) and seal with butyl rubber stoppers. Prior to inoculation, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- rsulfac1_medium (CultureMech:003189)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.771205Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J844 |
| 2026-03-13T06:19:18.450390Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003189 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.317573+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "RSULFAC1 MEDIUM" to "rsulfac1_medium" for consistent matching |
| 2026-06-05T07:41:44.718402+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 22 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:11.098715+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:54.893958+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint fb2e88f48744bdddcaf567149e57cd64046ab285db24cedfacc0682751da0aa5 |