spirochaeta_medium

CultureMech:003198 bacterial COMPLEX LIQUID
Original name: SPIROCHAETA MEDIUM

Overview

External ontology
mediadive.medium:J852 (source link unavailable) — SPIROCHAETA MEDIUM
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=852

Composition

flowchart LR
    CultureMech_003198["spirochaeta_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003198 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003198 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003198 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003198 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_NaCl["NaCl"]
    CultureMech_003198 --> ing4_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing4_NaCl -.-> CHEBI_26710
    ing5_Yeast_extract["Yeast extract"]
    CultureMech_003198 --> ing5_Yeast_extract
    ing6_Sucrose["Sucrose"]
    CultureMech_003198 --> ing6_Sucrose
    CHEBI_17992["CHEBI:17992"]
    ing6_Sucrose -.-> CHEBI_17992
    ing7_Maltose["Maltose"]
    CultureMech_003198 --> ing7_Maltose
    CHEBI_17306["CHEBI:17306"]
    ing7_Maltose -.-> CHEBI_17306
    ing8_NaHCO3["NaHCO3"]
    CultureMech_003198 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_Resazurin["Resazurin"]
    CultureMech_003198 --> ing9_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing9_Resazurin -.-> CHEBI_8806
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003198 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003198 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003198 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003198 --> ing13_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003198 --> ing14_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing15_ZnCl2["ZnCl2"]
    CultureMech_003198 --> ing15_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing15_ZnCl2 -.-> CHEBI_49976
    ing16_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003198 --> ing16_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing16_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing17_H3BO3["H3BO3"]
    CultureMech_003198 --> ing17_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing17_H3BO3 -.-> CHEBI_33118
    ing18_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003198 --> ing18_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing18_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing19_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003198 --> ing19_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing19_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing20_AlCl3["AlCl3"]
    CultureMech_003198 --> ing20_AlCl3
    CHEBI_30114["CHEBI:30114"]
    ing20_AlCl3 -.-> CHEBI_30114
    ing21_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003198 --> ing21_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing21_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing22_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003198 --> ing22_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing22_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing23_Biotin["Biotin"]
    CultureMech_003198 --> ing23_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing23_Biotin -.-> CHEBI_15956
    ing24_Folic_acid["Folic acid"]
    CultureMech_003198 --> ing24_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing24_Folic_acid -.-> CHEBI_27470
    ing25_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003198 --> ing25_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing25_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing26_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003198 --> ing26_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing26_Thiamine_HCl -.-> CHEBI_49105
    ing27_Riboflavin["Riboflavin"]
    CultureMech_003198 --> ing27_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing27_Riboflavin -.-> CHEBI_17015
    ing28_Nicotinamide["Nicotinamide"]
    CultureMech_003198 --> ing28_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing28_Nicotinamide -.-> CHEBI_17154
    ing29_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003198 --> ing29_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing29_DL_Calcium_pantothenate -.-> CHEBI_31345
    CultureMech_003198 --> more["...3 more"]

Ingredients (33)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.0996016 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
3.98406 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.996016 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 NH4Cl
Cl.H4N 53.492 g/mol
0.498008 G_PER_L CHEBI:31206
ammonium chloride
5 NaCl
Cl.Na 58.443 g/mol
24.9004 G_PER_L CHEBI:26710
sodium chloride
6 Yeast extract 0.996016 G_PER_L
7 Sucrose
C12H22O11 342.297 g/mol
1.69323 G_PER_L CHEBI:17992
sucrose
8 Maltose
C12H22O11 342.297 g/mol
1.69323 G_PER_L CHEBI:17306
maltose
9 NaHCO3
CHO3.Na 84.006 g/mol
1.99203 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 Resazurin
C12H7NO4 229.191 g/mol
0.000996016 G_PER_L CHEBI:8806
Resazurin
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.298805 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
0.298805 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
1.99 G_PER_L CHEBI:86249
iron dichloride tetrahydrate
14 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.198 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
15 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.13 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
16 ZnCl2
2Cl.Zn 136.296 g/mol
0.136 G_PER_L CHEBI:49976
zinc dichloride
17 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.0017 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
18 H3BO3
H3BO3 61.833 g/mol
0.0062 G_PER_L CHEBI:33118
boric acid
19 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.024 G_PER_L CHEBI:75213
sodium molybdate dihydrate
20 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.013 G_PER_L CHEBI:34887
nickel dichloride
21 AlCl3
AlCl3 133.341 g/mol
0.013 G_PER_L CHEBI:30114
aluminium trichloride
22 Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
0.0017 G_PER_L CHEBI:131361
disodium selenite pentahydrate
23 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.0033 G_PER_L CHEBI:63939
sodium tungstate dihydrate
24 Biotin
C10H16N2O3S 244.316 g/mol
0.0049 G_PER_L CHEBI:15956
biotin
25 Folic acid
C19H19N7O6 441.404 g/mol
0.0088 G_PER_L CHEBI:27470
folic acid
26 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.0041 G_PER_L CHEBI:30961
pyridoxine hydrochloride
27 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.0067 G_PER_L CHEBI:49105
thiamine hydrochloride
28 Riboflavin
C17H20N4O6 376.369 g/mol
0.0075 G_PER_L CHEBI:17015
riboflavin
29 Nicotinamide
C6H6N2O 122.127 g/mol
0.0025 G_PER_L CHEBI:17154
nicotinamide
30 DL-Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.0095 G_PER_L CHEBI:31345
Calcium pantothenate
31 Vitamin B12 0.0271 G_PER_L CHEBI:176843
vitamin B12
32 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.0027 G_PER_L CHEBI:30753
4-aminobenzoic acid
33 Lipoic acid
C8H14O2S2 206.332 g/mol
0.0041 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. For vial cultures, dissolve all compounds except Na2S x 9H2O and L-cysteine x HCl x H2O, then, adjust pH to 7.5. Dispense 19 ml of medium into 50-ml vials, purge with N2-CO2 (80:20) gas and fix with a stopper. After autoclaving, add filter-sterilized Na2S x 9H2O and L-cysteine x HCl x H2O solution to each vial as reducing agents.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.798647Z mediadive-import Imported from MediaDive Source: JCM, ID: J852
2026-03-13T06:19:18.523533Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003198
2026-03-17T06:30:38.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.400723+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "SPIROCHAETA MEDIUM" to "spirochaeta_medium" for consistent matching
2026-06-05T07:41:46.324870+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:11.633461+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:56.083103+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint e024fce674bb51be5ffe07c8fa1ffb816bf1cfebf5d43dae2d665242c22b7631