pyrolobus_medium

CultureMech:003204 archaea DEFINED LIQUID
Original name: PYROLOBUS MEDIUM

Overview

External ontology
mediadive.medium:J859 (source link unavailable) — PYROLOBUS MEDIUM
Applications
  • Microbial cultivation
pH
5.7
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=859

Composition

flowchart LR
    CultureMech_003204["pyrolobus_medium"]
    ing0_MES["MES"]
    CultureMech_003204 --> ing0_MES
    CHEBI_39010["CHEBI:39010"]
    ing0_MES -.-> CHEBI_39010
    ing1_Resazurin["Resazurin"]
    CultureMech_003204 --> ing1_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing1_Resazurin -.-> CHEBI_8806
    ing2_KNO3["KNO3"]
    CultureMech_003204 --> ing2_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing2_KNO3 -.-> CHEBI_63043
    ing3_KH2PO4["KH2PO4"]
    CultureMech_003204 --> ing3_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing3_KH2PO4 -.-> CHEBI_63036
    ing4_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003204 --> ing4_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing4_Na2S_x_9_H2O -.-> CHEBI_76209
    ing5_NaCl["NaCl"]
    CultureMech_003204 --> ing5_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing5_NaCl -.-> CHEBI_26710
    ing6_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003204 --> ing6_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing6_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing7_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003204 --> ing7_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing7_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing8_KCl["KCl"]
    CultureMech_003204 --> ing8_KCl
    CHEBI_32588["CHEBI:32588"]
    ing8_KCl -.-> CHEBI_32588
    ing9_NaBr["NaBr"]
    CultureMech_003204 --> ing9_NaBr
    CHEBI_63004["CHEBI:63004"]
    ing9_NaBr -.-> CHEBI_63004
    ing10_H3BO3["H3BO3"]
    CultureMech_003204 --> ing10_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing10_H3BO3 -.-> CHEBI_33118
    ing11_SrCl2_x_6_H2O["SrCl2 x 6 H2O"]
    CultureMech_003204 --> ing11_SrCl2_x_6_H2O
    CHEBI_36385["CHEBI:36385"]
    ing11_SrCl2_x_6_H2O -.-> CHEBI_36385
    ing12_KI["KI"]
    CultureMech_003204 --> ing12_KI
    CHEBI_8346["CHEBI:8346"]
    ing12_KI -.-> CHEBI_8346
    ing13_Trisodium_citrate["Trisodium citrate"]
    CultureMech_003204 --> ing13_Trisodium_citrate
    CHEBI_53258["CHEBI:53258"]
    ing13_Trisodium_citrate -.-> CHEBI_53258
    ing14_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003204 --> ing14_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing14_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing15_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003204 --> ing15_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing15_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing16_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003204 --> ing16_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing16_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing17_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003204 --> ing17_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing17_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing18_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003204 --> ing18_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing18_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing19_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003204 --> ing19_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing19_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing20_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_003204 --> ing20_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing20_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003204 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing22__NH4_2Ni_SO4_2_x_6_H2O["(NH4)2Ni(SO4)2 x 6 H2O"]
    CultureMech_003204 --> ing22__NH4_2Ni_SO4_2_x_6_H2O
    CHEBI_86149["CHEBI:86149"]
    ing22__NH4_2Ni_SO4_2_x_6_H2O -.-> CHEBI_86149
    ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003204 --> ing23_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing24_Na2SeO4["Na2SeO4"]
    CultureMech_003204 --> ing24_Na2SeO4
    CHEBI_77775["CHEBI:77775"]
    ing24_Na2SeO4 -.-> CHEBI_77775

Ingredients (25)

# Ingredient Concentration Ontology Notes
1 MES 1.94805 G_PER_L CHEBI:39010
MES
2 Resazurin
C12H7NO4 229.191 g/mol
0.0004995 G_PER_L CHEBI:8806
Resazurin
3 KNO3
K.NO3 101.102 g/mol
100 G_PER_L CHEBI:63043
potassium nitrate
4 KH2PO4
H2O4P.K 136.084 g/mol
50 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
5 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
15 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
6 NaCl
Cl.Na 58.443 g/mol
65.4 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 55.4, 10.0]
7 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
44.0 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 14.0, 30.0]
8 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
11 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
9 KCl
Cl.K 74.551 g/mol
1.3 G_PER_L CHEBI:32588
potassium chloride
10 NaBr
Br.Na 102.894 g/mol
0.2 G_PER_L CHEBI:63004
sodium bromide
11 H3BO3
H3BO3 61.833 g/mol
0.16 G_PER_L CHEBI:33118
boric acid
 [Merged 2 duplicates: 0.06, 0.1]
12 SrCl2 x 6 H2O
Cl2Sr.6H2O 266.616 g/mol
0.03 G_PER_L CHEBI:36385
strontium dichloride hexahydrate
13 KI
I.K 166.002 g/mol
0.0001 G_PER_L CHEBI:8346
potassium iodide
14 Trisodium citrate
C6H5O7.3Na 258.069 g/mol
0.02 G_PER_L CHEBI:53258
sodium citrate
15 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
2.5 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 1.5, 1.0]
16 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
5 G_PER_L CHEBI:86360
manganese(II) sulfate
17 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
18 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
1.8 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
19 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
1.8 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
20 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.1 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
21 AlK(SO4)2 x 12 H2O
Al.12H2O.K.2O4S 474.386 g/mol
0.18 G_PER_L CHEBI:86465
potassium aluminium sulfate dodecahydrate
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.1 G_PER_L CHEBI:75213
sodium molybdate dihydrate
23 (NH4)2Ni(SO4)2 x 6 H2O
6H2O.2H4N.Ni.2O4S 394.987 g/mol
2.8 G_PER_L CHEBI:86149
ammonium nickel sulfate hexahydrate
24 Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
0.1 G_PER_L CHEBI:63939
sodium tungstate dihydrate
25 Na2SeO4
2Na.O4Se 188.936 g/mol
0.1 G_PER_L CHEBI:77775
sodium selenate

Preparation

  1. Mix components of solution A, adjust pH to 5.7, then boil for 1 min and cool down to room temperature under a stream of H2-CO2 (4:1, v/v) gas mixture. Dispense the medium into culture vessels (e.g., 20 ml in 120 ml serum bottles) under the same gas mixture, seal with butyl rubber stoppers and autoclave. Separately autoclave Solutions B and C under a N2 atmosphere. Prior to inoculation, add Solutions B and C (10.0 ml each per liter of Solution A) and readjust pH to 5.7, if necessary. Pressurize inoculated bottles to 200 kPa H2-CO2 (4:1, v/v).
  2. First adjust pH to 1.0 with diluted H2SO4, then dissolve the salts.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:17.814707Z mediadive-import Imported from MediaDive Source: JCM, ID: J859
2026-03-13T03:09:08.839241Z data-quality-cleanup-v1.0 Data quality fixes Merged 4 duplicate ingredient(s)
2026-03-13T06:19:18.555035Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003204
2026-03-17T06:30:15.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:32.435655+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYROLOBUS MEDIUM" to "pyrolobus_medium" for consistent matching
2026-06-05T07:41:43.480983+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:35.788860+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:47:10.734958+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:32.667110+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint bb7c3cdba9a729f9acbfccc61665658d435e7f65ef2ffcb04ca00f41a22c4f72