Overview
- External ontology
- mediadive.medium:J861 (source link unavailable) — ACIDIFERROBACTER MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=861
Composition
flowchart LR
CultureMech_003207["acidiferrobacter_medium"]
ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003207 --> ing0_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
ing1_Sulfur["Sulfur"]
CultureMech_003207 --> ing1_Sulfur
CHEBI_26833["CHEBI:26833"]
ing1_Sulfur -.-> CHEBI_26833
ing2_FeSO4["FeSO4"]
CultureMech_003207 --> ing2_FeSO4
CHEBI_75832["CHEBI:75832"]
ing2_FeSO4 -.-> CHEBI_75832
ing3_Potassium_tetrathionate["Potassium tetrathionate"]
CultureMech_003207 --> ing3_Potassium_tetrathionate
CHEBI_86466["CHEBI:86466"]
ing3_Potassium_tetrathionate -.-> CHEBI_86466
ing4_Na2SO4["Na2SO4"]
CultureMech_003207 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5__NH4_2SO4["(NH4)2SO4"]
CultureMech_003207 --> ing5__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing5__NH4_2SO4 -.-> CHEBI_62946
ing6_KCl["KCl"]
CultureMech_003207 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_K2HPO4["K2HPO4"]
CultureMech_003207 --> ing7_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing7_K2HPO4 -.-> CHEBI_131527
ing8_Ca_NO3_2_x_4_H2O["Ca(NO3)2 x 4 H2O"]
CultureMech_003207 --> ing8_Ca_NO3_2_x_4_H2O
CHEBI_86159["CHEBI:86159"]
ing8_Ca_NO3_2_x_4_H2O -.-> CHEBI_86159
ing9_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003207 --> ing9_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing9_FeCl3_x_6_H2O -.-> CHEBI_86254
ing10_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003207 --> ing10_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing10_CuSO4_x_5_H2O -.-> CHEBI_31440
ing11_H3BO3["H3BO3"]
CultureMech_003207 --> ing11_H3BO3
CHEBI_33118["CHEBI:33118"]
ing11_H3BO3 -.-> CHEBI_33118
ing12_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003207 --> ing12_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing12_MnSO4_x_n_H2O -.-> CHEBI_86360
ing13_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003207 --> ing13_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing13_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing14_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003207 --> ing14_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing14_CoCl2_x_6_H2O -.-> CHEBI_53503
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003207 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
27.75 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 2 |
Sulfur
S 32.067 g/mol
|
1.04167 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 3 |
FeSO4
Fe.O4S 151.908 g/mol
|
10 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 4 |
Potassium tetrathionate
2K.O6S4 302.458 g/mol
|
5 G_PER_L |
CHEBI:86466
potassium tetrathionate
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
1.5 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
4.5 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.5 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.5 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 9 |
Ca(NO3)2 x 4 H2O
Ca.4H2O.2NO3 236.146 g/mol
|
0.14 G_PER_L |
CHEBI:86159
calcium nitrate tetrahydrate
|
|
| 10 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
1.1 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 11 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.05 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 12 |
H3BO3
H3BO3 61.833 g/mol
|
0.2 G_PER_L |
CHEBI:33118
boric acid
|
|
| 13 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 14 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.08 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 15 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.06 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.09 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Preparation
- Mix thoroughly except sulfur, adjust pH 1.9 with H2SO4 and autoclave. Steam sulfur for 3 hr on each of 3 successive days. After cooling, add sulfur and the following solutions (filter-sterilized) to the medium:
- Comment: For cultivation of JCM 30830, adjust pH of the medium to 1.7.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- acidiferrobacter_medium (CultureMech:003207)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.822384Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J861 |
| 2026-03-13T06:19:18.575183Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003207 |
| 2026-03-17T06:30:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.458441+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ACIDIFERROBACTER MEDIUM" to "acidiferrobacter_medium" for consistent matching |
| 2026-06-05T07:40:47.184925+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:52.551907+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:21.793726+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9d43544adf2973847323cb169fdbf05d287b94c2ae34e55886feb6d278088d8d |