steroidobacter_medium

Overview

External ontology

mediadive.medium:J862 (source link unavailable) — STEROIDOBACTER MEDIUM

Applications

• Microbial cultivation

pH

7.2

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=862

Composition

flowchart LR
    CultureMech_003208["steroidobacter_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003208 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003208 --> ing1_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing2_KH2PO4["KH2PO4"]
    CultureMech_003208 --> ing2_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing2_KH2PO4 -.-> CHEBI_63036
    ing3_KCl["KCl"]
    CultureMech_003208 --> ing3_KCl
    CHEBI_32588["CHEBI:32588"]
    ing3_KCl -.-> CHEBI_32588
    ing4_NH4Cl["NH4Cl"]
    CultureMech_003208 --> ing4_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing4_NH4Cl -.-> CHEBI_31206
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003208 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_Na2SO4["Na2SO4"]
    CultureMech_003208 --> ing6_Na2SO4
    CHEBI_32149["CHEBI:32149"]
    ing6_Na2SO4 -.-> CHEBI_32149
    ing7_NaNO3["NaNO3"]
    CultureMech_003208 --> ing7_NaNO3
    CHEBI_63005["CHEBI:63005"]
    ing7_NaNO3 -.-> CHEBI_63005
    ing8_NaOH["NaOH"]
    CultureMech_003208 --> ing8_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing8_NaOH -.-> CHEBI_32145
    ing9_Na2SeO3_x_5_H2O["Na2SeO3 x 5 H2O"]
    CultureMech_003208 --> ing9_Na2SeO3_x_5_H2O
    CHEBI_131361["CHEBI:131361"]
    ing9_Na2SeO3_x_5_H2O -.-> CHEBI_131361
    ing10_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
    CultureMech_003208 --> ing10_Na2WO4_x_2_H2O
    CHEBI_63939["CHEBI:63939"]
    ing10_Na2WO4_x_2_H2O -.-> CHEBI_63939
    ing11_Vitamin_B12["Vitamin B12"]
    CultureMech_003208 --> ing11_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing11_Vitamin_B12 -.-> CHEBI_176843
    ing12_Pantothenic_acid["Pantothenic acid"]
    CultureMech_003208 --> ing12_Pantothenic_acid
    CHEBI_7916["CHEBI:7916"]
    ing12_Pantothenic_acid -.-> CHEBI_7916
    ing13_Riboflavin["Riboflavin"]
    CultureMech_003208 --> ing13_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing13_Riboflavin -.-> CHEBI_17015
    ing14_Pyridoxamine_hydrochloride["Pyridoxamine hydrochloride"]
    CultureMech_003208 --> ing14_Pyridoxamine_hydrochloride
    CHEBI_131531["CHEBI:131531"]
    ing14_Pyridoxamine_hydrochloride -.-> CHEBI_131531
    ing15_Biotin["Biotin"]
    CultureMech_003208 --> ing15_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing15_Biotin -.-> CHEBI_15956
    ing16_Folic_acid["Folic acid"]
    CultureMech_003208 --> ing16_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing16_Folic_acid -.-> CHEBI_27470
    ing17_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003208 --> ing17_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing17_Nicotinic_acid -.-> CHEBI_15940
    ing18_Nicotinamide["Nicotinamide"]
    CultureMech_003208 --> ing18_Nicotinamide
    CHEBI_17154["CHEBI:17154"]
    ing18_Nicotinamide -.-> CHEBI_17154
    ing19_alpha_lipoic_acid["alpha-lipoic acid"]
    CultureMech_003208 --> ing19_alpha_lipoic_acid
    CHEBI_16494["CHEBI:16494"]
    ing19_alpha_lipoic_acid -.-> CHEBI_16494
    ing20_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003208 --> ing20_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing20_p_Aminobenzoic_acid -.-> CHEBI_30753
    ing21_Thiamine_HCl_x_2_H2O["Thiamine-HCl x 2 H2O"]
    CultureMech_003208 --> ing21_Thiamine_HCl_x_2_H2O
    CHEBI_132751["CHEBI:132751"]
    ing21_Thiamine_HCl_x_2_H2O -.-> CHEBI_132751

Ingredients (22)

Preparation

1. For growth with testosterone, dissolve testosterone in acetone (20 mg/ml) and dispense portions of the solution, e.g., 0.1 ml portions for 10 ml medium, to anaerobic culture tubes. Let the solvent evaporate to dryness. Dispense 10 ml portions of medium in the culture tubes and stream with N2-CO2 (80:20, v/v). Close the vessels and autoclave. Treat the vessels in an ultrasonic bath to detach and suspend the testosterone.
2. For growth with heptanoate, omit testosterone. Sterilize the gassed medium in anaerobic vessels by autoclaving. Add 2.5 mM heptanoate from a 20-fold filter-sterilized anaerobic stock solution after cooling.
3. After autoclaving, add (per 10 ml) 0.3 ml NaHCO3 solution (84 g/L, autoclaved under a CO2 atmosphere), 1 �l Trace element solution SL-10 (see Medium No. 433), 1 �l Selenite-tungstate solution (see below) and 1 �l Vitamin solution (see below). Adjust pH to 7.2. Incubate the cultures in the dark for up to 4 weeks. Briefly shake the cultures once per day.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• JCM_J862_STEROIDOBACTER_MEDIUM (CultureMech:003208)