Overview
- External ontology
- mediadive.medium:J866 (source link unavailable) — DESULFOTOMACULUM BS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=866
Composition
flowchart LR
CultureMech_003212["desulfotomaculum_bs_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003212 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_K2HPO4["K2HPO4"]
CultureMech_003212 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NH4Cl["NH4Cl"]
CultureMech_003212 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_NaCl["NaCl"]
CultureMech_003212 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_Na2SO4["Na2SO4"]
CultureMech_003212 --> ing4_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing4_Na2SO4 -.-> CHEBI_32149
ing5_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003212 --> ing5_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing5_MgCl2_x_6_H2O -.-> CHEBI_86345
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003212 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_KCl["KCl"]
CultureMech_003212 --> ing7_KCl
CHEBI_32588["CHEBI:32588"]
ing7_KCl -.-> CHEBI_32588
ing8_Resazurin["Resazurin"]
CultureMech_003212 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_NaHCO3["NaHCO3"]
CultureMech_003212 --> ing9_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing9_NaHCO3 -.-> CHEBI_32139
ing10_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003212 --> ing10_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing10_Sodium_pyruvate -.-> CHEBI_50144
ing11_Sodium_dithionite["Sodium dithionite"]
CultureMech_003212 --> ing11_Sodium_dithionite
CHEBI_66870["CHEBI:66870"]
ing11_Sodium_dithionite -.-> CHEBI_66870
ing12_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003212 --> ing12_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing12_Na2S_x_9_H2O -.-> CHEBI_76209
ing13_HCl["HCl"]
CultureMech_003212 --> ing13_HCl
CHEBI_17883["CHEBI:17883"]
ing13_HCl -.-> CHEBI_17883
ing14_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003212 --> ing14_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing14_FeCl2_x_4_H2O -.-> CHEBI_86249
ing15_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003212 --> ing15_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing15_CoCl2_x_6_H2O -.-> CHEBI_53503
ing16_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003212 --> ing16_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing16_MnCl2_x_4_H2O -.-> CHEBI_86368
ing17_ZnCl2["ZnCl2"]
CultureMech_003212 --> ing17_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing17_ZnCl2 -.-> CHEBI_49976
ing18_H3BO3["H3BO3"]
CultureMech_003212 --> ing18_H3BO3
CHEBI_33118["CHEBI:33118"]
ing18_H3BO3 -.-> CHEBI_33118
ing19_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003212 --> ing19_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing19_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing20_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003212 --> ing20_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing20_NiCl2_x_6_H2O -.-> CHEBI_34887
ing21_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003212 --> ing21_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing21_CuCl2_x_2_H2O -.-> CHEBI_86318
ing22_AlCl3["AlCl3"]
CultureMech_003212 --> ing22_AlCl3
CHEBI_30114["CHEBI:30114"]
ing22_AlCl3 -.-> CHEBI_30114
ing23_Na2WO4_x_2_H2O["Na2WO4 x 2 H2O"]
CultureMech_003212 --> ing23_Na2WO4_x_2_H2O
CHEBI_63939["CHEBI:63939"]
ing23_Na2WO4_x_2_H2O -.-> CHEBI_63939
ing24_Biotin["Biotin"]
CultureMech_003212 --> ing24_Biotin
CHEBI_15956["CHEBI:15956"]
ing24_Biotin -.-> CHEBI_15956
ing25_Folic_acid["Folic acid"]
CultureMech_003212 --> ing25_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing25_Folic_acid -.-> CHEBI_27470
ing26_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003212 --> ing26_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing26_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing27_Thiamine_HCl["Thiamine HCl"]
CultureMech_003212 --> ing27_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing27_Thiamine_HCl -.-> CHEBI_49105
ing28_Riboflavin["Riboflavin"]
CultureMech_003212 --> ing28_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing28_Riboflavin -.-> CHEBI_17015
ing29_Nicotinic_acid["Nicotinic acid"]
CultureMech_003212 --> ing29_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing29_Nicotinic_acid -.-> CHEBI_15940
CultureMech_003212 --> more["...4 more"]
Ingredients (34)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.318471 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
0.318471 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
1.06157 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
1.06157 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.18471 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 6 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.530786 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.106157 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
KCl
Cl.K 74.551 g/mol
|
0.106157 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00106157 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.25 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 11 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.2 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 12 |
Sodium dithionite
2Na.O4S2 174.11 g/mol
|
0.1 G_PER_L |
CHEBI:66870
sodium dithionite
|
|
| 13 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
0.04 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 14 |
HCl
HCl 36.461 g/mol
|
3.16832 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 15 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.9802 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 16 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.247525 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 17 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0990099 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 18 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.0693069 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 19 |
H3BO3
H3BO3 61.833 g/mol
|
0.00594059 G_PER_L |
CHEBI:33118
boric acid
|
|
| 20 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.039604 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 21 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.0693069 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 22 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.0019802 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 23 |
AlCl3
AlCl3 133.341 g/mol
|
0.0247525 G_PER_L |
CHEBI:30114
aluminium trichloride
|
|
| 24 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.00594059 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
|
| 25 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 26 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 27 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 28 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 29 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 30 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 31 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 32 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 33 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 34 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, adjust pH to 7.2 with KOH, distribute the medium into culture vessels (e.g., 9.4 ml in Balch tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, aseptically and anaerobically add the following solutions (per 9.4 ml medium, autoclaved or *filter-sterilized):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfotomaculum_bs_medium (CultureMech:003212)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.836943Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J866 |
| 2026-03-13T06:19:18.614295Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003212 |
| 2026-03-17T06:30:37.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.502926+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOTOMACULUM BS MEDIUM" to "desulfotomaculum_bs_medium" for consistent matching |
| 2026-06-05T07:40:57.529365+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 34 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.928372+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 5 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:31.937154+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9872336503180ae973cc90828a73f2074a16333cc354a6ad6d84863191eadf74 |