Overview
- External ontology
- mediadive.medium:J886 (source link unavailable) — MMA MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=886
Composition
flowchart LR
CultureMech_003234["mma_medium"]
ing0__NH4_2SO4["(NH4)2SO4"]
CultureMech_003234 --> ing0__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing0__NH4_2SO4 -.-> CHEBI_62946
ing1_K2HPO4["K2HPO4"]
CultureMech_003234 --> ing1_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing1_K2HPO4 -.-> CHEBI_131527
ing2_NaHCO3["NaHCO3"]
CultureMech_003234 --> ing2_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing2_NaHCO3 -.-> CHEBI_32139
ing3_HEPES["HEPES"]
CultureMech_003234 --> ing3_HEPES
CHEBI_46756["CHEBI:46756"]
ing3_HEPES -.-> CHEBI_46756
ing4_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003234 --> ing4_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing4_MgSO4_x_7_H2O -.-> CHEBI_31795
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003234 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003234 --> ing6_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing6_CuSO4_x_5_H2O -.-> CHEBI_31440
ing7_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003234 --> ing7_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing7_MnSO4_x_n_H2O -.-> CHEBI_86360
ing8_Fe_III__EDTA["Fe(III)-EDTA"]
CultureMech_003234 --> ing8_Fe_III__EDTA
CHEBI_30729["CHEBI:30729"]
ing8_Fe_III__EDTA -.-> CHEBI_30729
ing9_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003234 --> ing9_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing9_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing10_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003234 --> ing10_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing10_CoCl2_x_6_H2O -.-> CHEBI_53503
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003234 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
100 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 2 |
K2HPO4
HO4P.2K 174.174 g/mol
|
50 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 3 |
NaHCO3
CHO3.Na 84.006 g/mol
|
50 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 4 | HEPES | 1190 G_PER_L |
CHEBI:46756
HEPES
|
|
| 5 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
5 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.5 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.01 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 8 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.2 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 9 |
Fe(III)-EDTA
C10H12N2O8.Fe 344.057 g/mol
|
0.5 G_PER_L |
CHEBI:30729
ethylenediaminetetraacetatoferrate(1-)
|
|
| 10 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.005 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 11 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.0001 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.01 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L.
- Adjust pH to 7.8.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mma_medium (CultureMech:003234)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.902775Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J886 |
| 2026-03-13T06:19:18.809215Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003234 |
| 2026-03-17T06:30:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.684240+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MMA MEDIUM" to "mma_medium" for consistent matching |
| 2026-06-05T07:41:35.349802+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.249166+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:47.017058+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 72c26ef95792b18396d5bae858dd1f84d8ff79bb625ce9ac812e2856c5841007 |