Overview
- External ontology
- mediadive.medium:J888 (source link unavailable) — SALT POTATO-DEXTROSE AGAR (SPDA)
- Applications
-
- Microbial cultivation
- pH
- 5.5
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=888
Composition
flowchart LR
CultureMech_003236["salt_potato_dextrose_agar_spda"]
ing0_Potato["Potato"]
CultureMech_003236 --> ing0_Potato
ing1_Glucose["Glucose"]
CultureMech_003236 --> ing1_Glucose
CHEBI_17234["CHEBI:17234"]
ing1_Glucose -.-> CHEBI_17234
ing2_NaCl["NaCl"]
CultureMech_003236 --> ing2_NaCl
CHEBI_26710["CHEBI:26710"]
ing2_NaCl -.-> CHEBI_26710
ing3_Agar["Agar"]
CultureMech_003236 --> ing3_Agar
CHEBI_2509["CHEBI:2509"]
ing3_Agar -.-> CHEBI_2509
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Potato | 200 G_PER_L | ( peeled and cut) | |
| 2 |
Glucose
C6H12O6 180.156 g/mol
|
10 G_PER_L |
CHEBI:17234
glucose
|
|
| 3 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 4 | Agar | 15 G_PER_L |
CHEBI:2509
agar
|
Preparation
- Boil potatoes for 20 min. Strain through a fine sieve. Add glucose, NaCl and agar, and boil until dissolved. Do not use new potatoes. Adjust pH to 5.4 - 5.6.
- Comment: Commercial potato dextrose agar (BD-Difco or Oxoid) supplemented with NaCl can be used.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- salt_potato_dextrose_agar_spda (CultureMech:003236)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.909307Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J888 |
| 2026-03-13T06:19:18.823286Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003236 |
| 2026-03-17T06:30:30.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.700762+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "SALT POTATO-DEXTROSE AGAR (SPDA)" to "salt_potato_dextrose_agar_spda" for consistent matching |
| 2026-06-05T07:41:45.172154+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:55.211415+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint d2b0b0b0159d321dc3b93ff80f9bec4930e910ad847073fbaff4da45d45a768d |