Overview
- External ontology
- mediadive.medium:J893 (source link unavailable) — MBBS MEDIUM FOR HYDROGENOBACTER
- Applications
-
- Microbial cultivation
- pH
- 6.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=893
Composition
flowchart LR
CultureMech_003242["mbbs_medium_for_hydrogenobacter"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003242 --> ing0_Yeast_extract
ing1_NaHCO3["NaHCO3"]
CultureMech_003242 --> ing1_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing1_NaHCO3 -.-> CHEBI_32139
ing2_Sulfur["Sulfur"]
CultureMech_003242 --> ing2_Sulfur
CHEBI_26833["CHEBI:26833"]
ing2_Sulfur -.-> CHEBI_26833
ing3__NH4_2SO4["(NH4)2SO4"]
CultureMech_003242 --> ing3__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing3__NH4_2SO4 -.-> CHEBI_62946
ing4_KH2PO4["KH2PO4"]
CultureMech_003242 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003242 --> ing5_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing5_MgSO4_x_7_H2O -.-> CHEBI_31795
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003242 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003242 --> ing7_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing7_FeCl3_x_6_H2O -.-> CHEBI_86254
ing8_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003242 --> ing8_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing8_MnCl2_x_4_H2O -.-> CHEBI_86368
ing9_Na2B4O7_x_10_H2O["Na2B4O7 x 10 H2O"]
CultureMech_003242 --> ing9_Na2B4O7_x_10_H2O
CHEBI_131366["CHEBI:131366"]
ing9_Na2B4O7_x_10_H2O -.-> CHEBI_131366
ing10_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003242 --> ing10_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing10_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing11_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003242 --> ing11_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing11_CuCl2_x_2_H2O -.-> CHEBI_86318
ing12_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003242 --> ing12_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing12_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing13_VOSO4_x_n_H2O["VOSO4 x n H2O"]
CultureMech_003242 --> ing13_VOSO4_x_n_H2O
CHEBI_87020["CHEBI:87020"]
ing13_VOSO4_x_n_H2O -.-> CHEBI_87020
ing14_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
CultureMech_003242 --> ing14_CoSO4_x_7_H2O
CHEBI_91244["CHEBI:91244"]
ing14_CoSO4_x_7_H2O -.-> CHEBI_91244
Ingredients (15)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 |
NaHCO3
CHO3.Na 84.006 g/mol
|
10 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 3 |
Sulfur
S 32.067 g/mol
|
0.985222 G_PER_L |
CHEBI:26833
sulfur atom
|
|
| 4 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
1.3 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.28 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.25 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.07 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.002 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 9 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.0018 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 10 |
Na2B4O7 x 10 H2O
10H2O.H4B4O9.2Na 417.401 g/mol
|
0.0045 G_PER_L |
CHEBI:131366
disodium tetraborate decahydrate
|
|
| 11 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.00022 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 12 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
5e-05 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 13 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
3e-05 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 14 |
VOSO4 x n H2O
(H2O)n.O5SV 181.021 g/mol
|
3e-05 G_PER_L |
CHEBI:87020
vanadyl sulfate hydrate
|
|
| 15 |
CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
|
1e-05 G_PER_L |
CHEBI:91244
cobalt(2+) sulfate heptahydrate
|
Preparation
- Autoclave the base solution and the yeast extract solution separately. Filter-sterilize the NaHCO3 solution and steam sulfur for 3 hr on each of 3 successive 3 days. Aseptically mix the three solutions and distribute into culture vessels containing sulfur under a H2-CO2-O2 (80:20:2, v/v) gas stream and seal with butyl rubber stoppers. Adjust pH to 6.0 with sterile 1 N H2SO4, if necessary. Pressurize inoculated bottles to 100 kPa with the same gas mixture.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mbbs_medium_for_hydrogenobacter (CultureMech:003242)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.924645Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J893 |
| 2026-03-13T06:19:18.865041Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003242 |
| 2026-03-17T06:29:45.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.748086+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MBBS MEDIUM FOR HYDROGENOBACTER" to "mbbs_medium_for_hydrogenobacter" for consistent matching |
| 2026-06-05T07:41:13.342039+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 13 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:01.039493+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:42.277095+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint edfd67147b5fd6442d7e8bb0ea5f3d9387838481e4db3a981fa388a3cc51898d |