Overview
- External ontology
- mediadive.medium:J896 (source link unavailable) — MODIFIED BSK MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=896
Composition
flowchart LR
CultureMech_003244["modified_bsk_medium"]
ing0_HEPES["HEPES"]
CultureMech_003244 --> ing0_HEPES
CHEBI_46756["CHEBI:46756"]
ing0_HEPES -.-> CHEBI_46756
ing1_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003244 --> ing1_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing1_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing2_Glucose["Glucose"]
CultureMech_003244 --> ing2_Glucose
CHEBI_17234["CHEBI:17234"]
ing2_Glucose -.-> CHEBI_17234
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003244 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_N_Acetyl_D_glucosamine["N-Acetyl-D-glucosamine"]
CultureMech_003244 --> ing4_N_Acetyl_D_glucosamine
CHEBI_506227["CHEBI:506227"]
ing4_N_Acetyl_D_glucosamine -.-> CHEBI_506227
ing5_NaHCO3["NaHCO3"]
CultureMech_003244 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_MEM_alpha_Modification["MEM alpha Modification"]
CultureMech_003244 --> ing6_MEM_alpha_Modification
ing7_Yeast_extract["Yeast extract"]
CultureMech_003244 --> ing7_Yeast_extract
ing8_Probumin_Universal_Grade["Probumin Universal Grade"]
CultureMech_003244 --> ing8_Probumin_Universal_Grade
ing9_Neopeptone["Neopeptone"]
CultureMech_003244 --> ing9_Neopeptone
ing10_Rabbit_serum["Rabbit serum"]
CultureMech_003244 --> ing10_Rabbit_serum
Ingredients (11)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES | 75 G_PER_L |
CHEBI:46756
HEPES
|
|
| 2 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
8.75 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 3 |
Glucose
C6H12O6 180.156 g/mol
|
62.5 G_PER_L |
CHEBI:17234
glucose
|
|
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
10 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 5 |
N-Acetyl-D-glucosamine
C8H15NO6 221.209 g/mol
|
5 G_PER_L |
CHEBI:506227
N-acetyl-D-glucosamine
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
27.5 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 | MEM alpha Modification | 130 G_PER_L | ||
| 8 | Yeast extract | 25 G_PER_L | ||
| 9 | Probumin Universal Grade | 625 G_PER_L | ||
| 10 | Neopeptone | 62.5 G_PER_L | ||
| 11 | Rabbit serum | 40 G_PER_L |
Preparation
- Adjust pH to 7.2 with NaOH, fill up to 584 ml with distilled water and filter-sterilize with 0.22 �m PES filter.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- modified_bsk_medium (CultureMech:003244)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.930369Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J896 |
| 2026-01-28T19:48:13.981814Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.873475Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003244 |
| 2026-03-17T06:29:55.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.757882+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MODIFIED BSK MEDIUM" to "modified_bsk_medium" for consistent matching |
| 2026-06-05T07:41:36.397987+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 5 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:48.160073+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 578ddf1c5dc4d077abab5305a14e58b865e4f365713a32d175733313d217470b |