Overview
- External ontology
- mediadive.medium:J905 (source link unavailable) — 225M1 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 6.8
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=905
Composition
flowchart LR
CultureMech_003254["225m1_medium"]
ing0_Casamino_acids["Casamino acids"]
CultureMech_003254 --> ing0_Casamino_acids
ing1_Yeast_extract["Yeast extract"]
CultureMech_003254 --> ing1_Yeast_extract
ing2_Starch["Starch"]
CultureMech_003254 --> ing2_Starch
CHEBI_28017["CHEBI:28017"]
ing2_Starch -.-> CHEBI_28017
ing3_NaCl["NaCl"]
CultureMech_003254 --> ing3_NaCl
CHEBI_26710["CHEBI:26710"]
ing3_NaCl -.-> CHEBI_26710
ing4_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003254 --> ing4_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing4_MgCl2_x_6_H2O -.-> CHEBI_86345
ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003254 --> ing5_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
ing6_K2SO4["K2SO4"]
CultureMech_003254 --> ing6_K2SO4
CHEBI_32036["CHEBI:32036"]
ing6_K2SO4 -.-> CHEBI_32036
ing7_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003254 --> ing7_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing7_Sodium_pyruvate -.-> CHEBI_50144
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Casamino acids | 1 G_PER_L | ||
| 2 | Yeast extract | 1 G_PER_L | ||
| 3 | Starch | 2 G_PER_L |
CHEBI:28017
starch
|
|
| 4 |
NaCl
Cl.Na 58.443 g/mol
|
20 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 5 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
300 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 6 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.13 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 7 |
K2SO4
2K.O4S 174.259 g/mol
|
5 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 8 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.13 G_PER_L |
CHEBI:50144
sodium pyruvate
|
Preparation
- Add components to distilled water and bring volume to 1.0 L Adjust pH to 6.8 with 25% KOH. For preparation of solid medium, add 23.0 g/L Bacto agar (BD-Difco).
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 225m1_medium (CultureMech:003254)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.960518Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J905 |
| 2026-01-28T19:48:10.730627Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:18.954765Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003254 |
| 2026-03-17T06:29:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.850836+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "225M1 MEDIUM" to "225m1_medium" for consistent matching |
| 2026-06-05T07:40:15.694843+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 7 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-08T23:19:27.174952+00:00 | chebi-regrounding-v1.0 | Corrected CHEBI ingredient grounding (audit G21/G22) | Re-grounded 2 term reference(s) to the correct CHEBI (see reports/chebi_grounding_audit.md). Label-conditional fixes for pyridoxine/pyridoxamine, glycerol, MnSO4, Ca(NO3)2, selenate; casamino acids de-grounded (mixture, no single CHEBI). |
| 2026-06-10T07:46:42.524315+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:33.940279+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 69d8f8e9e5e34c0548b22575f9a10a4eac37b697d4d0f0a6b7e604eb0439d7eb |