Overview
- External ontology
- mediadive.medium:J906 (source link unavailable) — MK MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=906
Composition
flowchart LR
CultureMech_003255["mk_medium"]
ing0_Trisodium_citrate_x_2_H2O["Trisodium citrate x 2 H2O"]
CultureMech_003255 --> ing0_Trisodium_citrate_x_2_H2O
CHEBI_53258["CHEBI:53258"]
ing0_Trisodium_citrate_x_2_H2O -.-> CHEBI_53258
ing1_Tryptone["Tryptone"]
CultureMech_003255 --> ing1_Tryptone
ing2_Peptone["Peptone"]
CultureMech_003255 --> ing2_Peptone
ing3_KH2PO4["KH2PO4"]
CultureMech_003255 --> ing3_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing3_KH2PO4 -.-> CHEBI_63036
ing4_NH4Cl["NH4Cl"]
CultureMech_003255 --> ing4_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing4_NH4Cl -.-> CHEBI_31206
ing5_NaCl["NaCl"]
CultureMech_003255 --> ing5_NaCl
CHEBI_26710["CHEBI:26710"]
ing5_NaCl -.-> CHEBI_26710
ing6_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003255 --> ing6_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing6_MgCl2_x_6_H2O -.-> CHEBI_86345
ing7_K2SO4["K2SO4"]
CultureMech_003255 --> ing7_K2SO4
CHEBI_32036["CHEBI:32036"]
ing7_K2SO4 -.-> CHEBI_32036
ing8_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003255 --> ing8_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing8_FeCl2_x_4_H2O -.-> CHEBI_86249
ing9_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003255 --> ing9_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing9_MnCl2_x_4_H2O -.-> CHEBI_86368
ing10_Glycine_NaOH_buffer["Glycine-NaOH buffer"]
CultureMech_003255 --> ing10_Glycine_NaOH_buffer
ing11_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003255 --> ing11_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing11_ZnSO4_x_7_H2O -.-> CHEBI_32312
ing12_H3BO3["H3BO3"]
CultureMech_003255 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003255 --> ing13_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003255 --> ing14_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing14_CuCl2_x_2_H2O -.-> CHEBI_86318
ing15_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003255 --> ing15_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing15_NiCl2_x_6_H2O -.-> CHEBI_34887
ing16_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003255 --> ing16_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing16_Na2MoO4_x_2_H2O -.-> CHEBI_75213
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
Trisodium citrate x 2 H2O
C6H5O7.3Na 258.069 g/mol
|
9.90099 G_PER_L |
CHEBI:53258
sodium citrate
|
|
| 2 | Tryptone | 19.802 G_PER_L | ||
| 3 | Peptone | 9.90099 G_PER_L | ||
| 4 |
KH2PO4
H2O4P.K 136.084 g/mol
|
5.94059 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 5 |
NH4Cl
Cl.H4N 53.492 g/mol
|
5.94059 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 6 |
NaCl
Cl.Na 58.443 g/mol
|
1980.2 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 7 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
1.9802 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 8 |
K2SO4
2K.O4S 174.259 g/mol
|
198.02 G_PER_L |
CHEBI:32036
potassium sulfate
|
|
| 9 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
0.356436 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 10 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.03356436 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
[Merged 2 duplicates: 0.00356436, 0.03] |
| 11 | Glycine-NaOH buffer | 100 G_PER_L | ||
| 12 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.1 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.3 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.2 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 15 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.01 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 16 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.02 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 17 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.03 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
Preparation
- Add components to distilled water and bring volume to 900 ml. Adjust pH to 7.0 and autoclave. For preparation of solid medium, add 20.0 g/L Bacto agar (BD-Difco). Separately autoclave the following solution:
- After autoclaving, add the solution to the medium.
- Adjust pH to 3.6.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- mk_medium (CultureMech:003255)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:17.962728Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J906 |
| 2026-01-28T19:48:08.103866Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T03:08:39.985219Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:18.962259Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003255 |
| 2026-03-17T06:29:41.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.859045+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "MK MEDIUM" to "mk_medium" for consistent matching |
| 2026-06-05T07:41:35.166666+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:08.207376+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:46.827525+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 929c086d15b979525bf322570972d13c67aa01228765ed52a8718342a0acca94 |