Overview
- External ontology
- mediadive.medium:J90 (source link unavailable) — S6 MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.0
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=90
Composition
flowchart LR
CultureMech_003258["s6_medium"]
ing0_KH2PO4["KH2PO4"]
CultureMech_003258 --> ing0_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing0_KH2PO4 -.-> CHEBI_63036
ing1_Na2HPO4["Na2HPO4"]
CultureMech_003258 --> ing1_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing1_Na2HPO4 -.-> CHEBI_34683
ing2__NH4_2SO4["(NH4)2SO4"]
CultureMech_003258 --> ing2__NH4_2SO4
CHEBI_62946["CHEBI:62946"]
ing2__NH4_2SO4 -.-> CHEBI_62946
ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003258 --> ing3_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
ing4_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
CultureMech_003258 --> ing4_FeCl3_x_6_H2O
CHEBI_86254["CHEBI:86254"]
ing4_FeCl3_x_6_H2O -.-> CHEBI_86254
ing5_MnSO4_x_n_H2O["MnSO4 x n H2O"]
CultureMech_003258 --> ing5_MnSO4_x_n_H2O
CHEBI_86360["CHEBI:86360"]
ing5_MnSO4_x_n_H2O -.-> CHEBI_86360
ing6_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003258 --> ing6_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing6_CaCl2_x_2_H2O -.-> CHEBI_86158
ing7_Na2S2O3["Na2S2O3"]
CultureMech_003258 --> ing7_Na2S2O3
CHEBI_132112["CHEBI:132112"]
ing7_Na2S2O3 -.-> CHEBI_132112
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.8 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 2 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
1.2 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 3 |
(NH4)2SO4
2H4N.O4S 132.141 g/mol
|
0.1 G_PER_L |
CHEBI:62946
ammonium sulfate
|
|
| 4 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.1 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 5 |
FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
|
0.03 G_PER_L |
CHEBI:86254
iron trichloride hexahydrate
|
|
| 6 |
MnSO4 x n H2O
Mn.O4S 151.001 g/mol
|
0.03 G_PER_L |
CHEBI:86360
manganese(II) sulfate
|
|
| 7 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.04 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 8 |
Na2S2O3
2Na.O3S2 158.111 g/mol
|
100 G_PER_L |
CHEBI:132112
sodium thiosulfate
|
Preparation
- Adjust pH to 7.0.
- Separately sterilize Na2S2O3 solution by filtration. Aseptically add sterile Na2S2O3 solution to the medium.
- For preparation of solid medium, add 15.0 g/L agar.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- s6_medium (CultureMech:003258)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:15.713272Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J90 |
| 2026-03-13T06:19:18.996071Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003258 |
| 2026-03-17T06:30:12.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.897346+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "S6 MEDIUM" to "s6_medium" for consistent matching |
| 2026-06-05T07:41:44.932027+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:37:35.807438+00:00 | primary-term-regrounding-v1.0 | Re-grounded wrong primary term id (OAK-verified) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-06-10T07:47:11.182277+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:55.004881+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint deaf6ba9d3ffe38ec2c194e13571167656b3529cfef83caece52cd54b794533b |