Overview
- External ontology
- mediadive.medium:J918 (source link unavailable) — DESULFAMPLUS MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.3
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=918
Composition
flowchart LR
CultureMech_003265["desulfamplus_medium"]
ing0_NaCl["NaCl"]
CultureMech_003265 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003265 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_Na2SO4["Na2SO4"]
CultureMech_003265 --> ing2_Na2SO4
CHEBI_32149["CHEBI:32149"]
ing2_Na2SO4 -.-> CHEBI_32149
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003265 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KCl["KCl"]
CultureMech_003265 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_NH4Cl["NH4Cl"]
CultureMech_003265 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Sodium_fumarate["Sodium fumarate"]
CultureMech_003265 --> ing6_Sodium_fumarate
CHEBI_115156["CHEBI:115156"]
ing6_Sodium_fumarate -.-> CHEBI_115156
ing7_HEPES["HEPES"]
CultureMech_003265 --> ing7_HEPES
CHEBI_46756["CHEBI:46756"]
ing7_HEPES -.-> CHEBI_46756
ing8_Yeast_extract["Yeast extract"]
CultureMech_003265 --> ing8_Yeast_extract
ing9_Resazurin["Resazurin"]
CultureMech_003265 --> ing9_Resazurin
CHEBI_8806["CHEBI:8806"]
ing9_Resazurin -.-> CHEBI_8806
ing10_Potassium_phosphate_buffer["Potassium phosphate buffer"]
CultureMech_003265 --> ing10_Potassium_phosphate_buffer
ing11_NaHCO3["NaHCO3"]
CultureMech_003265 --> ing11_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing11_NaHCO3 -.-> CHEBI_32139
ing12_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003265 --> ing12_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing12_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing13_FeSO4_x_5_H2O["FeSO4 x 5 H2O"]
CultureMech_003265 --> ing13_FeSO4_x_5_H2O
CHEBI_75832["CHEBI:75832"]
ing13_FeSO4_x_5_H2O -.-> CHEBI_75832
ing14_Thiamine_HCl["Thiamine HCl"]
CultureMech_003265 --> ing14_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing14_Thiamine_HCl -.-> CHEBI_49105
ing15_Myo_inositol["Myo-inositol"]
CultureMech_003265 --> ing15_Myo_inositol
CHEBI_17268["CHEBI:17268"]
ing15_Myo_inositol -.-> CHEBI_17268
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003265 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
ing17_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003265 --> ing17_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing17_p_Aminobenzoic_acid -.-> CHEBI_30753
ing18_Vitamin_B12["Vitamin B12"]
CultureMech_003265 --> ing18_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing18_Vitamin_B12 -.-> CHEBI_176843
ing19_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003265 --> ing19_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing19_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_003265 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Biotin["Biotin"]
CultureMech_003265 --> ing21_Biotin
CHEBI_15956["CHEBI:15956"]
ing21_Biotin -.-> CHEBI_15956
ing22_Folic_acid["Folic acid"]
CultureMech_003265 --> ing22_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing22_Folic_acid -.-> CHEBI_27470
Ingredients (23)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
19.4553 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
5.83658 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
Na2SO4
2Na.O4S 142.043 g/mol
|
3.15175 G_PER_L |
CHEBI:32149
sodium sulfate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.972763 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.535019 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.291829 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
Sodium fumarate
C4H2O4.2Na 160.036 g/mol
|
0.972763 G_PER_L |
CHEBI:115156
disodium fumarate
|
|
| 8 | HEPES | 2.33463 G_PER_L |
CHEBI:46756
HEPES
|
|
| 9 | Yeast extract | 0.194553 G_PER_L | ||
| 10 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000389105 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 11 | Potassium phosphate buffer | 1.8 G_PER_L | ||
| 12 |
NaHCO3
CHO3.Na 84.006 g/mol
|
2.35 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 13 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
8 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
|
| 14 |
FeSO4 x 5 H2O
Fe.O4S 151.908 g/mol
|
10 G_PER_L |
CHEBI:75832
iron(2+) sulfate (anhydrous)
|
|
| 15 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.9 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 16 |
Myo-inositol
C6H12O6 180.156 g/mol
|
0.4 G_PER_L |
CHEBI:17268
myo-inositol
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.04 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.05 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 19 | Vitamin B12 | 0.05 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 20 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.04 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.04 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.001 G_PER_L |
CHEBI:15956
biotin
|
|
| 23 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.02 G_PER_L |
CHEBI:27470
folic acid
|
Preparation
- Mix components thoroughly and adjust pH to 7.0 with NaOH. Distribute the medium into culture vessels under a stream of a N2 gas, seal with butyl rubber stoppers, and autoclave. After cooling, aseptically and anaerobically add per liter the following solutions from anaerobic stock solutions (autoclaved or *filter-sterilized):
- Readjust pH to 7.2 - 7.5, if necessary, with sterile HCl or NaOH solution. Inoculate when the medium turns colorless.
- Comment: Optionally add the FeSO4 x 5H2O solution as described, for the formation of magnetosome.
- Use trace minerals (see Medium No. 151) with final 0.4 g/L Na2MoO4 x 2H2O, 0.02 g/L CuSO4 x 5H2O and 0.01 g/L NiCl2 x 6H2O.
- Comment: The medium tubes can be stocked under a N2 gas atmosphere. In this case, prior to use, fill the head space with air and allow to stand until the O2 gradient is formed.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- JCM_J918_DESULFAMPLUS_MEDIUM (CultureMech:003265)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.000127Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J918 |
| 2026-03-13T06:19:19.043841Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003265 |
| 2026-03-17T06:30:35.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:32.949442+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFAMPLUS MEDIUM" to "desulfamplus_medium" for consistent matching |
| 2026-06-05T07:40:17.916345+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 21 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:43.177015+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:37.503708+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint efd09effe25333955285eba3482c5a8fa36c313b2589938f3cea691797cc69af |