peat_medium_1_for_methanoregura_boonei

Overview

External ontology

mediadive.medium:J923 (source link unavailable) — PEAT MEDIUM 1 FOR METHANOREGURA BOONEI

Applications

• Microbial cultivation

pH

5.1

Notes

Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=923

Composition

flowchart LR
    CultureMech_003270["peat_medium_1_for_methanoregura_boonei"]
    ing0_HOMOPIPES["HOMOPIPES"]
    CultureMech_003270 --> ing0_HOMOPIPES
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003270 --> ing1_Yeast_extract
    ing2_Coenzyme_M["Coenzyme M"]
    CultureMech_003270 --> ing2_Coenzyme_M
    CHEBI_17905["CHEBI:17905"]
    ing2_Coenzyme_M -.-> CHEBI_17905
    ing3_Sodium_acetate["Sodium acetate"]
    CultureMech_003270 --> ing3_Sodium_acetate
    CHEBI_32954["CHEBI:32954"]
    ing3_Sodium_acetate -.-> CHEBI_32954
    ing4_KCl["KCl"]
    CultureMech_003270 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_KH2PO4["KH2PO4"]
    CultureMech_003270 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_NH4Cl["NH4Cl"]
    CultureMech_003270 --> ing6_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing6_NH4Cl -.-> CHEBI_31206
    ing7_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003270 --> ing7_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing7_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing8_ZnCl2["ZnCl2"]
    CultureMech_003270 --> ing8_ZnCl2
    CHEBI_49976["CHEBI:49976"]
    ing8_ZnCl2 -.-> CHEBI_49976
    ing9_H3BO3["H3BO3"]
    CultureMech_003270 --> ing9_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing9_H3BO3 -.-> CHEBI_33118
    ing10_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003270 --> ing10_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing10_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing11_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003270 --> ing11_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing11_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing12_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
    CultureMech_003270 --> ing12_FeCl2_x_4_H2O
    CHEBI_86249["CHEBI:86249"]
    ing12_FeCl2_x_4_H2O -.-> CHEBI_86249
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003270 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003270 --> ing14_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing14_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing15_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003270 --> ing15_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing15_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing16_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003270 --> ing16_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing16_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing17_AlK_SO4_2_x_12_H2O["AlK(SO4)2 x 12 H2O"]
    CultureMech_003270 --> ing17_AlK_SO4_2_x_12_H2O
    CHEBI_86465["CHEBI:86465"]
    ing17_AlK_SO4_2_x_12_H2O -.-> CHEBI_86465
    ing18_Tris_base["Tris base"]
    CultureMech_003270 --> ing18_Tris_base
    CHEBI_9754["CHEBI:9754"]
    ing18_Tris_base -.-> CHEBI_9754
    ing19_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003270 --> ing19_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing19_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing20_TiCl3["TiCl3"]
    CultureMech_003270 --> ing20_TiCl3
    ing21_Biotin["Biotin"]
    CultureMech_003270 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_003270 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003270 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003270 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_003270 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003270 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_DL_Calcium_pantothenate["DL-Calcium pantothenate"]
    CultureMech_003270 --> ing27_DL_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_DL_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_003270 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003270 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_003270 --> more["...1 more"]

Ingredients (31)

Preparation

1. Mix components thoroughly and adjust pH to 5.0 with HCl. Bring to a boil for 5 min, then, cool down under a stream of a N2-CO2 (80:20, v/v) gas mixture. Distribute the medium into Balch-type culture tubes (5 ml each) under the same gas mixture, seal with thick blue butyl rubber stoppers and autoclave.
2. To complete the medium, replace the gas phase with H2-CO2 (80:20, v/v) gas mixture, and add the following solutions to each of the culture tubes (containing 5 ml medium) from sterile anaerobic stocks (autoclaved or *filter-sterilized):
3. Check the final pH of the medium to be about 5.1. Pressurize inoculated tubes to 70 kPa H2-CO2 (80:20, v/v).
4. Filter-sterilize and store under N2 at 4C in the dark.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

• peat_medium_1_for_methanoregura_boonei (CultureMech:003270)