halanaerobium_sehlinense_medium

CultureMech:003276 bacterial COMPLEX LIQUID
Original name: HALANAEROBIUM SEHLINENSE MEDIUM

Overview

External ontology
mediadive.medium:J929 (source link unavailable) — HALANAEROBIUM SEHLINENSE MEDIUM
Applications
  • Microbial cultivation
pH
8.3
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=929

Composition

flowchart LR
    CultureMech_003276["halanaerobium_sehlinense_medium"]
    ing0_KH2PO4["KH2PO4"]
    CultureMech_003276 --> ing0_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing0_KH2PO4 -.-> CHEBI_63036
    ing1_K2HPO4["K2HPO4"]
    CultureMech_003276 --> ing1_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing1_K2HPO4 -.-> CHEBI_131527
    ing2_NH4Cl["NH4Cl"]
    CultureMech_003276 --> ing2_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing2_NH4Cl -.-> CHEBI_31206
    ing3_NaCl["NaCl"]
    CultureMech_003276 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_KCl["KCl"]
    CultureMech_003276 --> ing4_KCl
    CHEBI_32588["CHEBI:32588"]
    ing4_KCl -.-> CHEBI_32588
    ing5_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003276 --> ing5_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing5_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing6_KNO3["KNO3"]
    CultureMech_003276 --> ing6_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing6_KNO3 -.-> CHEBI_63043
    ing7_Yeast_extract["Yeast extract"]
    CultureMech_003276 --> ing7_Yeast_extract
    ing8_NaHCO3["NaHCO3"]
    CultureMech_003276 --> ing8_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing8_NaHCO3 -.-> CHEBI_32139
    ing9_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
    CultureMech_003276 --> ing9_MgCl2_x_6_H2O
    CHEBI_86345["CHEBI:86345"]
    ing9_MgCl2_x_6_H2O -.-> CHEBI_86345
    ing10_Glucose["Glucose"]
    CultureMech_003276 --> ing10_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing10_Glucose -.-> CHEBI_17234
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003276 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003276 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003276 --> ing13_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing13_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing14_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003276 --> ing14_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing14_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing15_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003276 --> ing15_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing15_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing16_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003276 --> ing16_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing16_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing17_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003276 --> ing17_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing17_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing18_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003276 --> ing18_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing18_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing19_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003276 --> ing19_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing19_AlK_SO4_2 -.-> CHEBI_86463
    ing20_H3BO3["H3BO3"]
    CultureMech_003276 --> ing20_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing20_H3BO3 -.-> CHEBI_33118
    ing21_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003276 --> ing21_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing21_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (22)

# Ingredient Concentration Ontology Notes
1 KH2PO4
H2O4P.K 136.084 g/mol
0.278293 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
2 K2HPO4
HO4P.2K 174.174 g/mol
0.278293 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
3 NH4Cl
Cl.H4N 53.492 g/mol
0.927644 G_PER_L CHEBI:31206
ammonium chloride
4 NaCl
Cl.Na 58.443 g/mol
186.529 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 185.529, 1.0]
5 KCl
Cl.K 74.551 g/mol
0.0927644 G_PER_L CHEBI:32588
potassium chloride
6 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.1927644 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.0927644, 0.1]
7 KNO3
K.NO3 101.102 g/mol
1.85529 G_PER_L CHEBI:63043
potassium nitrate
8 Yeast extract 0.927644 G_PER_L
9 NaHCO3
CHO3.Na 84.006 g/mol
25 G_PER_L CHEBI:32139
sodium hydrogencarbonate
10 MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
15 G_PER_L CHEBI:86345
magnesium dichloride hexahydrate
11 Glucose
C6H12O6 180.156 g/mol
20 G_PER_L CHEBI:17234
glucose
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
8 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
3 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
15 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
16 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
17 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
18 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
19 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
20 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
21 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
22 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Add components to distilled water and bring volume to 1.0 L. Adjust pH to 8.3 with 10 M KOH. Bring to a boil and cool down under a N2 gas stream. Then, distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave at 110C for 20 min. Aseptically and anaerobically add the following solutions (autoclaved or *filter-sterilized) into the medium (per liter):
  2. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.035630Z mediadive-import Imported from MediaDive Source: JCM, ID: J929
2026-03-13T03:09:11.153566Z data-quality-cleanup-v1.0 Data quality fixes Merged 2 duplicate ingredient(s)
2026-03-13T06:19:19.132822Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003276
2026-03-17T06:30:18.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.047437+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "HALANAEROBIUM SEHLINENSE MEDIUM" to "halanaerobium_sehlinense_medium" for consistent matching
2026-06-05T07:41:07.090671+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 19 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.932168+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:59.296311+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 6 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:36.900473+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ec2311ecf8376e0f2484c582c7c8e811b78e8e07b610335dd1a7485766a63562