Overview
- External ontology
- mediadive.medium:J946 (source link unavailable) — KORTHOF'S MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=946
Composition
flowchart LR
CultureMech_003294["korthofs_medium"]
ing0_Rabbit_serum["Rabbit serum"]
CultureMech_003294 --> ing0_Rabbit_serum
ing1_NaCl["NaCl"]
CultureMech_003294 --> ing1_NaCl
CHEBI_26710["CHEBI:26710"]
ing1_NaCl -.-> CHEBI_26710
ing2_Na2HPO4["Na2HPO4"]
CultureMech_003294 --> ing2_Na2HPO4
CHEBI_34683["CHEBI:34683"]
ing2_Na2HPO4 -.-> CHEBI_34683
ing3_Polypeptone["Polypeptone"]
CultureMech_003294 --> ing3_Polypeptone
ing4_KH2PO4["KH2PO4"]
CultureMech_003294 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NaHCO3["NaHCO3"]
CultureMech_003294 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_KCl["KCl"]
CultureMech_003294 --> ing6_KCl
CHEBI_32588["CHEBI:32588"]
ing6_KCl -.-> CHEBI_32588
ing7_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003294 --> ing7_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing7_CaCl2_x_2_H2O -.-> CHEBI_86158
Ingredients (8)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Rabbit serum | 50 G_PER_L | ||
| 2 |
NaCl
Cl.Na 58.443 g/mol
|
15.5556 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 3 |
Na2HPO4
HO4P.2Na 141.958 g/mol
|
9.77778 G_PER_L |
CHEBI:34683
disodium hydrogenphosphate
|
|
| 4 | Polypeptone | 8.88889 G_PER_L | ||
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.66667 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
0.222222 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
KCl
Cl.K 74.551 g/mol
|
0.444444 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 8 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.444444 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
Preparation
- Autoclave distilled water. After autoclaving, allow to cool, aseptically add 10 x Korthof's basal medium and heat-inactivated rabbit serum that has been filter-sterilized ahead of time.
- Mix ingredients except CaCl2 and autoclave. Dissolve CaCl2 in 50 ml distilled water and filter-sterilize. After autoclaving, allow to cool, add sterile CaCl2 solution to the medium.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- korthofs_medium (CultureMech:003294)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.092123Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J946 |
| 2026-03-13T06:19:19.276785Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003294 |
| 2026-03-17T06:30:21.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.209128+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "KORTHOF'S MEDIUM" to "korthofs_medium" for consistent matching |
| 2026-06-05T07:41:10.488504+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 6 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:00.403007+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:39.714098+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint bfaea4a7d31b812ef76031d09055c883256b416e8903bb22cbdaa88bdb2497fd |