Overview
- External ontology
- mediadive.medium:J954 (source link unavailable) — NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM NO.3
- Applications
-
- Microbial cultivation
- pH
- 7.0
- High-metal flag
- True
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=954
Composition
flowchart LR
CultureMech_003302["neutral_oligotrophic_haloarchaeal_medium_no_3"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003302 --> ing0_Yeast_extract
ing1_Fish_peptone["Fish peptone"]
CultureMech_003302 --> ing1_Fish_peptone
ing2_Sodium_formate["Sodium formate"]
CultureMech_003302 --> ing2_Sodium_formate
CHEBI_62965["CHEBI:62965"]
ing2_Sodium_formate -.-> CHEBI_62965
ing3_Sodium_acetate["Sodium acetate"]
CultureMech_003302 --> ing3_Sodium_acetate
CHEBI_32954["CHEBI:32954"]
ing3_Sodium_acetate -.-> CHEBI_32954
ing4_Sodium_lactate["Sodium lactate"]
CultureMech_003302 --> ing4_Sodium_lactate
CHEBI_75228["CHEBI:75228"]
ing4_Sodium_lactate -.-> CHEBI_75228
ing5_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003302 --> ing5_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing5_Sodium_pyruvate -.-> CHEBI_50144
ing6_NH4Cl["NH4Cl"]
CultureMech_003302 --> ing6_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing6_NH4Cl -.-> CHEBI_31206
ing7_NaCl["NaCl"]
CultureMech_003302 --> ing7_NaCl
CHEBI_26710["CHEBI:26710"]
ing7_NaCl -.-> CHEBI_26710
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003302 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003302 --> ing9_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
ing10_KCl["KCl"]
CultureMech_003302 --> ing10_KCl
CHEBI_32588["CHEBI:32588"]
ing10_KCl -.-> CHEBI_32588
ing11_CaCl2["CaCl2"]
CultureMech_003302 --> ing11_CaCl2
CHEBI_3312["CHEBI:3312"]
ing11_CaCl2 -.-> CHEBI_3312
ing12_K2HPO4["K2HPO4"]
CultureMech_003302 --> ing12_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing12_K2HPO4 -.-> CHEBI_131527
ing13_KH2PO4["KH2PO4"]
CultureMech_003302 --> ing13_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing13_KH2PO4 -.-> CHEBI_63036
Ingredients (14)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 0.993049 G_PER_L | ||
| 2 | Fish peptone | 0.248262 G_PER_L | ||
| 3 |
Sodium formate
CHO2.Na 68.007 g/mol
|
0.248262 G_PER_L |
CHEBI:62965
sodium formate
|
|
| 4 |
Sodium acetate
C2H3O2.Na 82.034 g/mol
|
0.248262 G_PER_L |
CHEBI:32954
sodium acetate
|
|
| 5 |
Sodium lactate
C3H5O3.Na 112.06 g/mol
|
0.248262 G_PER_L |
CHEBI:75228
sodium lactate
|
|
| 6 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
0.248262 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 7 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.26745 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 8 |
NaCl
Cl.Na 58.443 g/mol
|
240 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
30 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
35 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 11 |
KCl
Cl.K 74.551 g/mol
|
7 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 12 |
CaCl2
Ca.2Cl 110.984 g/mol
|
0.5549 G_PER_L |
CHEBI:3312
calcium dichloride
|
|
| 13 |
K2HPO4
HO4P.2K 174.174 g/mol
|
14.5263 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 14 |
KH2PO4
H2O4P.K 136.084 g/mol
|
2.25901 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
Preparation
- Mix components thoroughly and adjust pH to 7.0. For preparation of solid medium, add 20.0 g/L agar. (if necessary, use washed agar as described in the recipe of Medium No. 574). Autoclave and, after cooling, add the following solutions (*filter-sterilized or autoclaved) to the medium.
- Add components to distilled water and bring volume to 1.0 L. Adjust pH to 7.5 with 1 M Tris base.
- Combine the two solutions and check that pH is close to 7.5. Add an equal volume of distilled water.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- neutral_oligotrophic_haloarchaeal_medium_no_3 (CultureMech:003302)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.119673Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J954 |
| 2026-03-13T06:19:19.331781Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003302 |
| 2026-03-17T06:30:04.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.271961+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "NEUTRAL OLIGOTROPHIC HALOARCHAEAL MEDIUM NO.3" to "neutral_oligotrophic_haloarchaeal_medium_no_3" for consistent matching |
| 2026-06-05T07:41:39.608743+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:09.528933+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:32.120733+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 6aabd4ce4573420c07e75a720f21172a51ed38780142a7d1ffcb1e4d108d1337 |