Overview
- External ontology
- mediadive.medium:J963 (source link unavailable) — DESULFOTOMACULUM TONGENSE MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=963
Composition
flowchart LR
CultureMech_003311["desulfotomaculum_tongense_medium"]
ing0_Sea_Salt["Sea Salt"]
CultureMech_003311 --> ing0_Sea_Salt
ing1_KH2PO4["KH2PO4"]
CultureMech_003311 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_NH4Cl["NH4Cl"]
CultureMech_003311 --> ing2_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing2_NH4Cl -.-> CHEBI_31206
ing3_Sodium_pyruvate["Sodium pyruvate"]
CultureMech_003311 --> ing3_Sodium_pyruvate
CHEBI_50144["CHEBI:50144"]
ing3_Sodium_pyruvate -.-> CHEBI_50144
ing4_Resazurin["Resazurin"]
CultureMech_003311 --> ing4_Resazurin
CHEBI_8806["CHEBI:8806"]
ing4_Resazurin -.-> CHEBI_8806
ing5_NaHCO3["NaHCO3"]
CultureMech_003311 --> ing5_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing5_NaHCO3 -.-> CHEBI_32139
ing6_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003311 --> ing6_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing6_Na2S_x_9_H2O -.-> CHEBI_76209
ing7_Biotin["Biotin"]
CultureMech_003311 --> ing7_Biotin
CHEBI_15956["CHEBI:15956"]
ing7_Biotin -.-> CHEBI_15956
ing8_Folic_acid["Folic acid"]
CultureMech_003311 --> ing8_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing8_Folic_acid -.-> CHEBI_27470
ing9_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003311 --> ing9_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing9_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing10_Thiamine_HCl["Thiamine HCl"]
CultureMech_003311 --> ing10_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing10_Thiamine_HCl -.-> CHEBI_49105
ing11_Riboflavin["Riboflavin"]
CultureMech_003311 --> ing11_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing11_Riboflavin -.-> CHEBI_17015
ing12_Nicotinic_acid["Nicotinic acid"]
CultureMech_003311 --> ing12_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing12_Nicotinic_acid -.-> CHEBI_15940
ing13_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003311 --> ing13_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing13_Calcium_pantothenate -.-> CHEBI_31345
ing14_Vitamin_B12["Vitamin B12"]
CultureMech_003311 --> ing14_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing14_Vitamin_B12 -.-> CHEBI_176843
ing15_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003311 --> ing15_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing15_p_Aminobenzoic_acid -.-> CHEBI_30753
ing16_Lipoic_acid["Lipoic acid"]
CultureMech_003311 --> ing16_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing16_Lipoic_acid -.-> CHEBI_16494
Ingredients (17)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Sea Salt | 28.8462 G_PER_L | ||
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.134615 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.240385 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 4 |
Sodium pyruvate
C3H3O3.Na 110.044 g/mol
|
1.05769 G_PER_L |
CHEBI:50144
sodium pyruvate
|
|
| 5 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000961538 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 6 |
NaHCO3
CHO3.Na 84.006 g/mol
|
20 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 7 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
10 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 8 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 9 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 10 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 11 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 12 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 13 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 14 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 15 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 16 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 17 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
Preparation
- Mix components thoroughly, bring to a boil and cool down to room temperature under a N2-CO2 (4:1, v/v) gas stream. Dispense the medium into culture vessels under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter the following solutions from anaerobic stocks (autoclaved or *filter-sterilized):
- Check final pH to be 7.0 - 7.2.
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- desulfotomaculum_tongense_medium (CultureMech:003311)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.147575Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J963 |
| 2026-03-13T06:19:19.401944Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003311 |
| 2026-03-17T06:30:57.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.353870+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DESULFOTOMACULUM TONGENSE MEDIUM" to "desulfotomaculum_tongense_medium" for consistent matching |
| 2026-06-05T07:40:57.638455+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 16 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:55.972965+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:32.010646+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 96e7cdd385552e2bc38b43f8d6e90b49b5a4ec653e009bb12b798ead2c9480a3 |