Overview
- External ontology
- mediadive.medium:J964 (source link unavailable) — 480G MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=964
Composition
flowchart LR
CultureMech_003312["480g_medium"]
ing0_NaCl["NaCl"]
CultureMech_003312 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_NH4Cl["NH4Cl"]
CultureMech_003312 --> ing1_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing1_NH4Cl -.-> CHEBI_31206
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003312 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003312 --> ing3_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing3_MgCl2_x_6_H2O -.-> CHEBI_86345
ing4_KCl["KCl"]
CultureMech_003312 --> ing4_KCl
CHEBI_32588["CHEBI:32588"]
ing4_KCl -.-> CHEBI_32588
ing5_KH2PO4["KH2PO4"]
CultureMech_003312 --> ing5_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing5_KH2PO4 -.-> CHEBI_63036
ing6_Casitone["Casitone"]
CultureMech_003312 --> ing6_Casitone
ing7_Yeast_extract["Yeast extract"]
CultureMech_003312 --> ing7_Yeast_extract
ing8_Resazurin["Resazurin"]
CultureMech_003312 --> ing8_Resazurin
CHEBI_8806["CHEBI:8806"]
ing8_Resazurin -.-> CHEBI_8806
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003312 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Co_NO3_2_x_6_H2O["Co(NO3)2 x 6 H2O"]
CultureMech_003312 --> ing10_Co_NO3_2_x_6_H2O
CHEBI_86214["CHEBI:86214"]
ing10_Co_NO3_2_x_6_H2O -.-> CHEBI_86214
ing11_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
CultureMech_003312 --> ing11_CuSO4_x_5_H2O
CHEBI_31440["CHEBI:31440"]
ing11_CuSO4_x_5_H2O -.-> CHEBI_31440
ing12_H3BO3["H3BO3"]
CultureMech_003312 --> ing12_H3BO3
CHEBI_33118["CHEBI:33118"]
ing12_H3BO3 -.-> CHEBI_33118
ing13_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003312 --> ing13_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing13_MnCl2_x_4_H2O -.-> CHEBI_86368
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003312 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
CultureMech_003312 --> ing15_ZnSO4_x_7_H2O
CHEBI_32312["CHEBI:32312"]
ing15_ZnSO4_x_7_H2O -.-> CHEBI_32312
Ingredients (16)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.497512 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.328358 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.149254 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.348259 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 5 |
KCl
Cl.K 74.551 g/mol
|
0.298507 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 6 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.298507 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 7 | Casitone | 0.995025 G_PER_L | ||
| 8 | Yeast extract | 0.497512 G_PER_L | ||
| 9 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000995025 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
5 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Co(NO3)2 x 6 H2O
Co.6H2O.2NO3 291.031 g/mol
|
0.00494 G_PER_L |
CHEBI:86214
cobalt dinitrate hexahydrate
|
|
| 12 |
CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
|
0.0079 G_PER_L |
CHEBI:31440
copper(II) sulfate pentahydrate
|
|
| 13 |
H3BO3
H3BO3 61.833 g/mol
|
0.286 G_PER_L |
CHEBI:33118
boric acid
|
|
| 14 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.181 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.039 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
|
0.0222 G_PER_L |
CHEBI:32312
zinc sulfate heptahydrate
|
Preparation
- Autoclave under a N2 gas atmosphere. After cooling, add per liter the following solution (autoclaved and stored under a N2 gas atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- 480g_medium (CultureMech:003312)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.150706Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J964 |
| 2026-03-13T06:19:19.409471Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003312 |
| 2026-03-17T06:30:19.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.363042+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "480G MEDIUM" to "480g_medium" for consistent matching |
| 2026-06-05T07:40:15.831277+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 14 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:42.559880+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:34.119062+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 75581c2854b3ba30e38c9db9178f898c67e7a718aa8bedbe9b89fa745536e9ed |