Overview
- External ontology
- mediadive.medium:J965 (source link unavailable) — G-S MEDIUM
- Applications
-
- Microbial cultivation
- pH
- 7.4
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=965
Composition
flowchart LR
CultureMech_003313["g_s_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003313 --> ing0_Yeast_extract
ing1_KH2PO4["KH2PO4"]
CultureMech_003313 --> ing1_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing1_KH2PO4 -.-> CHEBI_63036
ing2_K2HPO4["K2HPO4"]
CultureMech_003313 --> ing2_K2HPO4
CHEBI_131527["CHEBI:131527"]
ing2_K2HPO4 -.-> CHEBI_131527
ing3_Urea["Urea"]
CultureMech_003313 --> ing3_Urea
CHEBI_16199["CHEBI:16199"]
ing3_Urea -.-> CHEBI_16199
ing4_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
CultureMech_003313 --> ing4_L_Cysteine_HCl_x_H2O
CHEBI_91248["CHEBI:91248"]
ing4_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
ing5_MOPS["MOPS"]
CultureMech_003313 --> ing5_MOPS
CHEBI_39074["CHEBI:39074"]
ing5_MOPS -.-> CHEBI_39074
ing6_Cellobiose["Cellobiose"]
CultureMech_003313 --> ing6_Cellobiose
CHEBI_17057["CHEBI:17057"]
ing6_Cellobiose -.-> CHEBI_17057
ing7_Resazurin["Resazurin"]
CultureMech_003313 --> ing7_Resazurin
CHEBI_8806["CHEBI:8806"]
ing7_Resazurin -.-> CHEBI_8806
ing8_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003313 --> ing8_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing8_MgCl2_x_6_H2O -.-> CHEBI_86345
ing9_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003313 --> ing9_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing9_CaCl2_x_2_H2O -.-> CHEBI_86158
ing10_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003313 --> ing10_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing10_FeSO4_x_7_H2O -.-> CHEBI_75836
ing11_ZnCl2["ZnCl2"]
CultureMech_003313 --> ing11_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing11_ZnCl2 -.-> CHEBI_49976
Ingredients (12)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 4.45545 G_PER_L | ||
| 2 |
KH2PO4
H2O4P.K 136.084 g/mol
|
1.48515 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 3 |
K2HPO4
HO4P.2K 174.174 g/mol
|
2.87129 G_PER_L |
CHEBI:131527
dipotassium hydrogen phosphate
|
|
| 4 |
Urea
CH4N2O 60.056 g/mol
|
2.87129 G_PER_L |
CHEBI:16199
urea
|
|
| 5 |
L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
|
3.000003 G_PER_L |
CHEBI:91248
L-cysteine hydrochloride hydrate
|
[Merged 2 duplicates: 2.9703, 0.029703] |
| 6 | MOPS | 20.6931 G_PER_L |
CHEBI:39074
MOPS
|
|
| 7 |
Cellobiose
C12H22O11 342.297 g/mol
|
9.90099 G_PER_L |
CHEBI:17057
cellobiose
|
|
| 8 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.000990099 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 9 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
2.47525 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 10 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.371287 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 11 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.0306931 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 12 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.00990099 G_PER_L |
CHEBI:49976
zinc dichloride
|
Preparation
- Mix components thoroughly and adjust pH to 7.4. Distribute the medium into culture vessels under a N2-CO2 (9:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add per liter 1 ml following sterile solution from anaerobic stock.
- <I>Mineral solution</I>:
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- g_s_medium (CultureMech:003313)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.153549Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J965 |
| 2026-03-13T03:08:38.454122Z | data-quality-cleanup-v1.0 | Data quality fixes | Merged 1 duplicate ingredient(s) |
| 2026-03-13T06:19:19.416288Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003313 |
| 2026-03-17T06:29:40.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.371244+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "G-S MEDIUM" to "g_s_medium" for consistent matching |
| 2026-06-05T07:41:05.860974+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 11 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:46:58.929700+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:05:35.877177+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 9a9ee8b7c8d395106029e6e7e5ab3c258dc3e8c5e8e91934cc510303177e8cf0 |