alkalinus_abyssiniese_medium

CultureMech:003323 bacterial COMPLEX LIQUID
Original name: ALKALINUS ABYSSINIESE MEDIUM

Overview

External ontology
mediadive.medium:J974 (source link unavailable) — ALKALINUS ABYSSINIESE MEDIUM
Applications
  • Microbial cultivation
High-metal flag
True
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=974

Composition

flowchart LR
    CultureMech_003323["alkalinus_abyssiniese_medium"]
    ing0_NaCl["NaCl"]
    CultureMech_003323 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_Yeast_extract["Yeast extract"]
    CultureMech_003323 --> ing1_Yeast_extract
    ing2_Peptone["Peptone"]
    CultureMech_003323 --> ing2_Peptone
    ing3_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003323 --> ing3_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing3_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003323 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_KH2PO4["KH2PO4"]
    CultureMech_003323 --> ing5_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing5_KH2PO4 -.-> CHEBI_63036
    ing6_Glucose["Glucose"]
    CultureMech_003323 --> ing6_Glucose
    CHEBI_17234["CHEBI:17234"]
    ing6_Glucose -.-> CHEBI_17234
    ing7_Na2CO3["Na2CO3"]
    CultureMech_003323 --> ing7_Na2CO3
    CHEBI_29377["CHEBI:29377"]
    ing7_Na2CO3 -.-> CHEBI_29377
    ing8_H3BO4["H3BO4"]
    CultureMech_003323 --> ing8_H3BO4
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003323 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003323 --> ing10_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing10_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing11_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003323 --> ing11_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing11_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing12_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003323 --> ing12_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing12_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing13_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003323 --> ing13_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing13_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing14_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003323 --> ing14_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing14_CuCl2_x_2_H2O -.-> CHEBI_86318

Ingredients (15)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
1017.39 G_PER_L CHEBI:26710
sodium chloride
2 Yeast extract 43.4783 G_PER_L
3 Peptone 43.4783 G_PER_L
4 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
1.73913 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
1.73913 G_PER_L CHEBI:86158
calcium chloride dihydrate
6 KH2PO4
H2O4P.K 136.084 g/mol
0.869565 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
7 Glucose
C6H12O6 180.156 g/mol
5 G_PER_L CHEBI:17234
glucose
8 Na2CO3
CO3.2Na 105.988 g/mol
100 G_PER_L CHEBI:29377
sodium carbonate
9 H3BO4 0.03 G_PER_L
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.01 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.003 G_PER_L CHEBI:75213
sodium molybdate dihydrate
12 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.003 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
13 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.002 G_PER_L CHEBI:34887
nickel dichloride
14 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.01 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
15 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.001 G_PER_L CHEBI:86318
copper(II) chloride dihydrate

Preparation

  1. Add components to distilled water and bring volume to 900 ml. For preparation of solid medium, add 20.0 g/L agar. Autoclave and cool (to 50 - 60C for agar medium). Then, add aseptically the following solutions (separately autoclaved):

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.182192Z mediadive-import Imported from MediaDive Source: JCM, ID: J974
2026-03-13T06:19:19.493073Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003323
2026-03-17T06:30:25.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.498683+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "ALKALINUS ABYSSINIESE MEDIUM" to "alkalinus_abyssiniese_medium" for consistent matching
2026-06-05T07:40:47.953227+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 12 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:52.818248+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:22.452548+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint d204ef7e6256f7070a11b53fb90f3a3aa32d43eb8d8060bcf5e1ac097affe028