Overview
- External ontology
- mediadive.medium:J985 (source link unavailable) — METHANOMETHYLOVORANS UPONENSIS MEDIUM
- Applications
-
- Microbial cultivation
- Notes
- Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=985
Composition
flowchart LR
CultureMech_003334["methanomethylovorans_uponensis_medium"]
ing0_NaCl["NaCl"]
CultureMech_003334 --> ing0_NaCl
CHEBI_26710["CHEBI:26710"]
ing0_NaCl -.-> CHEBI_26710
ing1_KCl["KCl"]
CultureMech_003334 --> ing1_KCl
CHEBI_32588["CHEBI:32588"]
ing1_KCl -.-> CHEBI_32588
ing2_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003334 --> ing2_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing2_MgCl2_x_6_H2O -.-> CHEBI_86345
ing3_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003334 --> ing3_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing3_CaCl2_x_2_H2O -.-> CHEBI_86158
ing4_KH2PO4["KH2PO4"]
CultureMech_003334 --> ing4_KH2PO4
CHEBI_63036["CHEBI:63036"]
ing4_KH2PO4 -.-> CHEBI_63036
ing5_NH4Cl["NH4Cl"]
CultureMech_003334 --> ing5_NH4Cl
CHEBI_31206["CHEBI:31206"]
ing5_NH4Cl -.-> CHEBI_31206
ing6_Resazurin["Resazurin"]
CultureMech_003334 --> ing6_Resazurin
CHEBI_8806["CHEBI:8806"]
ing6_Resazurin -.-> CHEBI_8806
ing7_Trimethylamine_HCl["Trimethylamine-HCl"]
CultureMech_003334 --> ing7_Trimethylamine_HCl
CHEBI_64700["CHEBI:64700"]
ing7_Trimethylamine_HCl -.-> CHEBI_64700
ing8_NaHCO3["NaHCO3"]
CultureMech_003334 --> ing8_NaHCO3
CHEBI_32139["CHEBI:32139"]
ing8_NaHCO3 -.-> CHEBI_32139
ing9_Na2S_x_9_H2O["Na2S x 9 H2O"]
CultureMech_003334 --> ing9_Na2S_x_9_H2O
CHEBI_76209["CHEBI:76209"]
ing9_Na2S_x_9_H2O -.-> CHEBI_76209
ing10_Biotin["Biotin"]
CultureMech_003334 --> ing10_Biotin
CHEBI_15956["CHEBI:15956"]
ing10_Biotin -.-> CHEBI_15956
ing11_Folic_acid["Folic acid"]
CultureMech_003334 --> ing11_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing11_Folic_acid -.-> CHEBI_27470
ing12_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003334 --> ing12_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing12_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing13_Thiamine_HCl["Thiamine HCl"]
CultureMech_003334 --> ing13_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing13_Thiamine_HCl -.-> CHEBI_49105
ing14_Riboflavin["Riboflavin"]
CultureMech_003334 --> ing14_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing14_Riboflavin -.-> CHEBI_17015
ing15_Nicotinic_acid["Nicotinic acid"]
CultureMech_003334 --> ing15_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing15_Nicotinic_acid -.-> CHEBI_15940
ing16_Calcium_pantothenate["Calcium pantothenate"]
CultureMech_003334 --> ing16_Calcium_pantothenate
CHEBI_31345["CHEBI:31345"]
ing16_Calcium_pantothenate -.-> CHEBI_31345
ing17_Vitamin_B12["Vitamin B12"]
CultureMech_003334 --> ing17_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing17_Vitamin_B12 -.-> CHEBI_176843
ing18_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003334 --> ing18_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing18_p_Aminobenzoic_acid -.-> CHEBI_30753
ing19_Lipoic_acid["Lipoic acid"]
CultureMech_003334 --> ing19_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing19_Lipoic_acid -.-> CHEBI_16494
ing20_HCl["HCl"]
CultureMech_003334 --> ing20_HCl
CHEBI_17883["CHEBI:17883"]
ing20_HCl -.-> CHEBI_17883
ing21_FeCl2_x_4_H2O["FeCl2 x 4 H2O"]
CultureMech_003334 --> ing21_FeCl2_x_4_H2O
CHEBI_86249["CHEBI:86249"]
ing21_FeCl2_x_4_H2O -.-> CHEBI_86249
ing22_ZnCl2["ZnCl2"]
CultureMech_003334 --> ing22_ZnCl2
CHEBI_49976["CHEBI:49976"]
ing22_ZnCl2 -.-> CHEBI_49976
ing23_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003334 --> ing23_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing23_MnCl2_x_4_H2O -.-> CHEBI_86368
ing24_H3BO3["H3BO3"]
CultureMech_003334 --> ing24_H3BO3
CHEBI_33118["CHEBI:33118"]
ing24_H3BO3 -.-> CHEBI_33118
ing25_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003334 --> ing25_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing25_CoCl2_x_6_H2O -.-> CHEBI_53503
ing26_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
CultureMech_003334 --> ing26_CuCl2_x_2_H2O
CHEBI_86318["CHEBI:86318"]
ing26_CuCl2_x_2_H2O -.-> CHEBI_86318
ing27_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
CultureMech_003334 --> ing27_NiCl2_x_6_H2O
CHEBI_34887["CHEBI:34887"]
ing27_NiCl2_x_6_H2O -.-> CHEBI_34887
ing28_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003334 --> ing28_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing28_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing29_NaOH["NaOH"]
CultureMech_003334 --> ing29_NaOH
CHEBI_32145["CHEBI:32145"]
ing29_NaOH -.-> CHEBI_32145
CultureMech_003334 --> more["...2 more"]
Ingredients (32)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 |
NaCl
Cl.Na 58.443 g/mol
|
0.98912 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 2 |
KCl
Cl.K 74.551 g/mol
|
0.49456 G_PER_L |
CHEBI:32588
potassium chloride
|
|
| 3 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.395648 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 4 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.098912 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 5 |
KH2PO4
H2O4P.K 136.084 g/mol
|
0.197824 G_PER_L |
CHEBI:63036
potassium dihydrogen phosphate
|
|
| 6 |
NH4Cl
Cl.H4N 53.492 g/mol
|
0.49456 G_PER_L |
CHEBI:31206
ammonium chloride
|
|
| 7 |
Resazurin
C12H7NO4 229.191 g/mol
|
0.00098912 G_PER_L |
CHEBI:8806
Resazurin
|
|
| 8 |
Trimethylamine-HCl
C3H9N.HCl 95.573 g/mol
|
10 G_PER_L |
CHEBI:64700
trimethylamine hydrochloride
|
|
| 9 |
NaHCO3
CHO3.Na 84.006 g/mol
|
20 G_PER_L |
CHEBI:32139
sodium hydrogencarbonate
|
|
| 10 |
Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
|
8 G_PER_L |
CHEBI:76209
sodium sulfide nonahydrate
|
|
| 11 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 12 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 13 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 14 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 15 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 16 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 17 |
Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 18 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 19 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 20 |
Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 21 |
HCl
HCl 36.461 g/mol
|
2.5 G_PER_L |
CHEBI:17883
hydrogen chloride
|
|
| 22 |
FeCl2 x 4 H2O
Cl2Fe.4H2O 198.811 g/mol
|
1.5 G_PER_L |
CHEBI:86249
iron dichloride tetrahydrate
|
|
| 23 |
ZnCl2
2Cl.Zn 136.296 g/mol
|
0.07 G_PER_L |
CHEBI:49976
zinc dichloride
|
|
| 24 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 25 |
H3BO3
H3BO3 61.833 g/mol
|
0.006 G_PER_L |
CHEBI:33118
boric acid
|
|
| 26 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.19 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 27 |
CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
|
0.002 G_PER_L |
CHEBI:86318
copper(II) chloride dihydrate
|
|
| 28 |
NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
|
0.024 G_PER_L |
CHEBI:34887
nickel dichloride
|
|
| 29 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.036 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 30 |
NaOH
HO.Na 39.997 g/mol
|
0.4 G_PER_L |
CHEBI:32145
sodium hydroxide
|
|
| 31 |
Na2SeO3 x 5 H2O
5H2O.2Na.O3Se 263.012 g/mol
|
0.006 G_PER_L |
CHEBI:131361
disodium selenite pentahydrate
|
|
| 32 |
Na2WO4 x 2 H2O
2H2O.2Na.O4W 329.846 g/mol
|
0.008 G_PER_L |
CHEBI:63939
sodium tungstate dihydrate
|
Preparation
- Mix components thoroughly and autoclave under a N2-CO2 (80:20, v/v) gas atmosphere. After cooling, add the following solutions (autoclaved or *filter-sterilized):
- Aseptically and anaerobically distribute the medium into culture vessels under a N2-CO2 (80:20, v/v) gas mixture and seal with butyl rubber stoppers. Finally, add per liter the following solution from an anaerobic stock (autoclaved and stored under a N2 atmosphere):
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- methanomethylovorans_uponensis_medium (CultureMech:003334)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-26T17:53:18.220518Z | mediadive-import | Imported from MediaDive | Source: JCM, ID: J985 |
| 2026-01-28T19:48:31.542150Z | chebi-enrichment | Added CHEBI ontology terms to ingredients | Enriched using MicrobeMediaParam and MediaDive chemical mappings |
| 2026-03-13T06:19:19.588360Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003334 |
| 2026-03-17T06:30:32.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:33.613695+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "METHANOMETHYLOVORANS UPONENSIS MEDIUM" to "methanomethylovorans_uponensis_medium" for consistent matching |
| 2026-06-05T07:41:32.016078+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 32 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-06-10T07:47:07.155589+00:00 | primary-term-label-canonicalization-v1.0 | Canonicalized primary term label to OBO canonical (OAK) | 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi. |
| 2026-08-06T03:04:30.851882+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint f3586f80da019bbc1f26567677dc5d3a4567361d949cae23fd615e3160d17d28 |