diluted_nitrate_mineral_medium

CultureMech:003342 bacterial DEFINED LIQUID
Original name: DILUTED NITRATE MINERAL MEDIUM

Overview

External ontology
mediadive.medium:J992 (source link unavailable) — DILUTED NITRATE MINERAL MEDIUM
Applications
  • Microbial cultivation
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=992

Composition

flowchart LR
    CultureMech_003342["diluted_nitrate_mineral_medium"]
    ing0_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003342 --> ing0_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing0_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing1_KNO3["KNO3"]
    CultureMech_003342 --> ing1_KNO3
    CHEBI_63043["CHEBI:63043"]
    ing1_KNO3 -.-> CHEBI_63043
    ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003342 --> ing2_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing3_Na2HPO4["Na2HPO4"]
    CultureMech_003342 --> ing3_Na2HPO4
    CHEBI_34683["CHEBI:34683"]
    ing3_Na2HPO4 -.-> CHEBI_34683
    ing4_KH2PO4["KH2PO4"]
    CultureMech_003342 --> ing4_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing4_KH2PO4 -.-> CHEBI_63036
    ing5_Na2_EDTA["Na2-EDTA"]
    CultureMech_003342 --> ing5_Na2_EDTA
    CHEBI_64734["CHEBI:64734"]
    ing5_Na2_EDTA -.-> CHEBI_64734
    ing6_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003342 --> ing6_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing6_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing7_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003342 --> ing7_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing7_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing8_FeCl3_x_6_H2O["FeCl3 x 6 H2O"]
    CultureMech_003342 --> ing8_FeCl3_x_6_H2O
    CHEBI_86254["CHEBI:86254"]
    ing8_FeCl3_x_6_H2O -.-> CHEBI_86254
    ing9_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003342 --> ing9_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing9_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing10_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
    CultureMech_003342 --> ing10_MnCl2_x_4_H2O
    CHEBI_86368["CHEBI:86368"]
    ing10_MnCl2_x_4_H2O -.-> CHEBI_86368
    ing11_H3BO3["H3BO3"]
    CultureMech_003342 --> ing11_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing11_H3BO3 -.-> CHEBI_33118
    ing12_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
    CultureMech_003342 --> ing12_CoCl2_x_6_H2O
    CHEBI_53503["CHEBI:53503"]
    ing12_CoCl2_x_6_H2O -.-> CHEBI_53503
    ing13_CuCl2_x_2_H2O["CuCl2 x 2 H2O"]
    CultureMech_003342 --> ing13_CuCl2_x_2_H2O
    CHEBI_86318["CHEBI:86318"]
    ing13_CuCl2_x_2_H2O -.-> CHEBI_86318
    ing14_NiCl2_x_6_H2O["NiCl2 x 6 H2O"]
    CultureMech_003342 --> ing14_NiCl2_x_6_H2O
    CHEBI_34887["CHEBI:34887"]
    ing14_NiCl2_x_6_H2O -.-> CHEBI_34887
    ing15_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003342 --> ing15_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing15_Na2MoO4_x_2_H2O -.-> CHEBI_75213

Ingredients (16)

# Ingredient Concentration Ontology Notes
1 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.197824 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
2 KNO3
K.NO3 101.102 g/mol
0.197824 G_PER_L CHEBI:63043
potassium nitrate
3 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.0296736 G_PER_L CHEBI:86158
calcium chloride dihydrate
4 Na2HPO4
HO4P.2Na 141.958 g/mol
5 G_PER_L CHEBI:34683
disodium hydrogenphosphate
5 KH2PO4
H2O4P.K 136.084 g/mol
5 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
6 Na2-EDTA
C10H14N2O8.2Na 336.208 g/mol
0.872 G_PER_L CHEBI:64734
EDTA disodium salt (anhydrous)
 [Merged 2 duplicates: 0.5, 0.372]
7 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.2 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
8 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.25 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
9 FeCl3 x 6 H2O
Cl3Fe.6H2O 270.294 g/mol
0.27 G_PER_L CHEBI:86254
iron trichloride hexahydrate
10 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
11 MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
0.03 G_PER_L CHEBI:86368
manganese(II) chloride tetrahydrate
12 H3BO3
H3BO3 61.833 g/mol
0.3 G_PER_L CHEBI:33118
boric acid
13 CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
0.2 G_PER_L CHEBI:53503
cobalt chloride hexahydrate
14 CuCl2 x 2 H2O
Cl2Cu.2H2O 170.482 g/mol
0.01 G_PER_L CHEBI:86318
copper(II) chloride dihydrate
15 NiCl2 x 6 H2O
Cl2Ni 129.599 g/mol
0.02 G_PER_L CHEBI:34887
nickel dichloride
16 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.03 G_PER_L CHEBI:75213
sodium molybdate dihydrate

Preparation

  1. Mix components thoroughly. For preparation of agar medium, add 15.0 g/L agar. Autoclave and, after cooling to 60C, add the following solutions (filter-sterilized):
  2. pH of the medium should be around 6.8. Cultivate under an air containing methane in gastight flasks or jars leaving &;80% gas phase by volume (20% methane for liquid cultures; 50% methane for agar plate cultures).
  3. Adjust pH to 3.6.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.245989Z mediadive-import Imported from MediaDive Source: JCM, ID: J992
2026-03-13T03:09:26.966512Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s)
2026-03-13T06:19:19.657274Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003342
2026-03-17T06:30:36.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.693616+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "DILUTED NITRATE MINERAL MEDIUM" to "diluted_nitrate_mineral_medium" for consistent matching
2026-06-05T07:40:58.733865+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 15 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:37:34.732325+00:00 primary-term-regrounding-v1.0 Re-grounded wrong primary term id (OAK-verified) 1 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-06-10T07:46:56.380874+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 3 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:32.836378+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint ff6213e121f49592ae134b5b3e6c3c578e2e056cd165f9bc57475cc1a81bc284