mbm_medium_2

CultureMech:003343 bacterial DEFINED LIQUID
Original name: MBM MEDIUM 2

Overview

External ontology
mediadive.medium:J994 (source link unavailable) — MBM MEDIUM 2
Applications
  • Microbial cultivation
pH
7.5
Notes
Source: JCM | Link: https://www.jcm.riken.jp/cgi-bin/jcm/jcm_grmd?GRMD=994

Composition

flowchart LR
    CultureMech_003343["mbm_medium_2"]
    ing0_NaCl["NaCl"]
    CultureMech_003343 --> ing0_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing0_NaCl -.-> CHEBI_26710
    ing1_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003343 --> ing1_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing1_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing2_KCl["KCl"]
    CultureMech_003343 --> ing2_KCl
    CHEBI_32588["CHEBI:32588"]
    ing2_KCl -.-> CHEBI_32588
    ing3_NH4Cl["NH4Cl"]
    CultureMech_003343 --> ing3_NH4Cl
    CHEBI_31206["CHEBI:31206"]
    ing3_NH4Cl -.-> CHEBI_31206
    ing4_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003343 --> ing4_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing4_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing5_K2HPO4["K2HPO4"]
    CultureMech_003343 --> ing5_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing5_K2HPO4 -.-> CHEBI_131527
    ing6_Sodium_acetate_x_3_H2O["Sodium acetate x 3 H2O"]
    CultureMech_003343 --> ing6_Sodium_acetate_x_3_H2O
    CHEBI_32954["CHEBI:32954"]
    ing6_Sodium_acetate_x_3_H2O -.-> CHEBI_32954
    ing7_Resazurin["Resazurin"]
    CultureMech_003343 --> ing7_Resazurin
    CHEBI_8806["CHEBI:8806"]
    ing7_Resazurin -.-> CHEBI_8806
    ing8_Disodium_fumarate["Disodium fumarate"]
    CultureMech_003343 --> ing8_Disodium_fumarate
    CHEBI_115156["CHEBI:115156"]
    ing8_Disodium_fumarate -.-> CHEBI_115156
    ing9_NaHCO3["NaHCO3"]
    CultureMech_003343 --> ing9_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing9_NaHCO3 -.-> CHEBI_32139
    ing10_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003343 --> ing10_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing10_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing11_Na2S_x_9_H2O["Na2S x 9 H2O"]
    CultureMech_003343 --> ing11_Na2S_x_9_H2O
    CHEBI_76209["CHEBI:76209"]
    ing11_Na2S_x_9_H2O -.-> CHEBI_76209
    ing12_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
    CultureMech_003343 --> ing12_Nitrilotriacetic_acid
    CHEBI_44557["CHEBI:44557"]
    ing12_Nitrilotriacetic_acid -.-> CHEBI_44557
    ing13_MnSO4_x_n_H2O["MnSO4 x n H2O"]
    CultureMech_003343 --> ing13_MnSO4_x_n_H2O
    CHEBI_86360["CHEBI:86360"]
    ing13_MnSO4_x_n_H2O -.-> CHEBI_86360
    ing14_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
    CultureMech_003343 --> ing14_FeSO4_x_7_H2O
    CHEBI_75836["CHEBI:75836"]
    ing14_FeSO4_x_7_H2O -.-> CHEBI_75836
    ing15_CoSO4_x_7_H2O["CoSO4 x 7 H2O"]
    CultureMech_003343 --> ing15_CoSO4_x_7_H2O
    CHEBI_91244["CHEBI:91244"]
    ing15_CoSO4_x_7_H2O -.-> CHEBI_91244
    ing16_ZnSO4_x_7_H2O["ZnSO4 x 7 H2O"]
    CultureMech_003343 --> ing16_ZnSO4_x_7_H2O
    CHEBI_32312["CHEBI:32312"]
    ing16_ZnSO4_x_7_H2O -.-> CHEBI_32312
    ing17_CuSO4_x_5_H2O["CuSO4 x 5 H2O"]
    CultureMech_003343 --> ing17_CuSO4_x_5_H2O
    CHEBI_31440["CHEBI:31440"]
    ing17_CuSO4_x_5_H2O -.-> CHEBI_31440
    ing18_AlK_SO4_2["AlK(SO4)2"]
    CultureMech_003343 --> ing18_AlK_SO4_2
    CHEBI_86463["CHEBI:86463"]
    ing18_AlK_SO4_2 -.-> CHEBI_86463
    ing19_H3BO3["H3BO3"]
    CultureMech_003343 --> ing19_H3BO3
    CHEBI_33118["CHEBI:33118"]
    ing19_H3BO3 -.-> CHEBI_33118
    ing20_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
    CultureMech_003343 --> ing20_Na2MoO4_x_2_H2O
    CHEBI_75213["CHEBI:75213"]
    ing20_Na2MoO4_x_2_H2O -.-> CHEBI_75213
    ing21_Biotin["Biotin"]
    CultureMech_003343 --> ing21_Biotin
    CHEBI_15956["CHEBI:15956"]
    ing21_Biotin -.-> CHEBI_15956
    ing22_Folic_acid["Folic acid"]
    CultureMech_003343 --> ing22_Folic_acid
    CHEBI_27470["CHEBI:27470"]
    ing22_Folic_acid -.-> CHEBI_27470
    ing23_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
    CultureMech_003343 --> ing23_Pyridoxine_hydrochloride
    CHEBI_30961["CHEBI:30961"]
    ing23_Pyridoxine_hydrochloride -.-> CHEBI_30961
    ing24_Thiamine_HCl["Thiamine HCl"]
    CultureMech_003343 --> ing24_Thiamine_HCl
    CHEBI_49105["CHEBI:49105"]
    ing24_Thiamine_HCl -.-> CHEBI_49105
    ing25_Riboflavin["Riboflavin"]
    CultureMech_003343 --> ing25_Riboflavin
    CHEBI_17015["CHEBI:17015"]
    ing25_Riboflavin -.-> CHEBI_17015
    ing26_Nicotinic_acid["Nicotinic acid"]
    CultureMech_003343 --> ing26_Nicotinic_acid
    CHEBI_15940["CHEBI:15940"]
    ing26_Nicotinic_acid -.-> CHEBI_15940
    ing27_Calcium_pantothenate["Calcium pantothenate"]
    CultureMech_003343 --> ing27_Calcium_pantothenate
    CHEBI_31345["CHEBI:31345"]
    ing27_Calcium_pantothenate -.-> CHEBI_31345
    ing28_Vitamin_B12["Vitamin B12"]
    CultureMech_003343 --> ing28_Vitamin_B12
    CHEBI_176843["CHEBI:176843"]
    ing28_Vitamin_B12 -.-> CHEBI_176843
    ing29_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
    CultureMech_003343 --> ing29_p_Aminobenzoic_acid
    CHEBI_30753["CHEBI:30753"]
    ing29_p_Aminobenzoic_acid -.-> CHEBI_30753
    CultureMech_003343 --> more["...1 more"]

Ingredients (31)

# Ingredient Concentration Ontology Notes
1 NaCl
Cl.Na 58.443 g/mol
79.2779 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 78.2779, 1.0]
2 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
8.870840000000001 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
 [Merged 2 duplicates: 5.87084, 3.0]
3 KCl
Cl.K 74.551 g/mol
1.46771 G_PER_L CHEBI:32588
potassium chloride
4 NH4Cl
Cl.H4N 53.492 g/mol
0.978474 G_PER_L CHEBI:31206
ammonium chloride
5 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.49138899999999996 G_PER_L CHEBI:86158
calcium chloride dihydrate
 [Merged 2 duplicates: 0.391389, 0.1]
6 K2HPO4
HO4P.2K 174.174 g/mol
0.391389 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
7 Sodium acetate x 3 H2O
C2H3O2.Na 82.034 g/mol
1.56556 G_PER_L CHEBI:32954
sodium acetate
8 Resazurin
C12H7NO4 229.191 g/mol
0.000978474 G_PER_L CHEBI:8806
Resazurin
9 Disodium fumarate
C4H2O4.2Na 160.036 g/mol
40 G_PER_L CHEBI:115156
disodium fumarate
10 NaHCO3
CHO3.Na 84.006 g/mol
50 G_PER_L CHEBI:32139
sodium hydrogencarbonate
11 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
6 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
12 Na2S x 9 H2O
9H2O.2Na.S 240.182 g/mol
6 G_PER_L CHEBI:76209
sodium sulfide nonahydrate
13 Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
1.5 G_PER_L CHEBI:44557
nitrilotriacetic acid
14 MnSO4 x n H2O
Mn.O4S 151.001 g/mol
0.5 G_PER_L CHEBI:86360
manganese(II) sulfate
15 FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
0.1 G_PER_L CHEBI:75836
iron(2+) sulfate heptahydrate
16 CoSO4 x 7 H2O
Co.7H2O.O4S 281.101 g/mol
0.1 G_PER_L CHEBI:91244
cobalt(2+) sulfate heptahydrate
17 ZnSO4 x 7 H2O
7H2O.O4S.Zn 287.558 g/mol
0.1 G_PER_L CHEBI:32312
zinc sulfate heptahydrate
18 CuSO4 x 5 H2O
Cu.5H2O.O4S 249.684 g/mol
0.01 G_PER_L CHEBI:31440
copper(II) sulfate pentahydrate
19 AlK(SO4)2
Al.K.2O4S 258.206 g/mol
0.01 G_PER_L CHEBI:86463
potassium aluminium sulfate
20 H3BO3
H3BO3 61.833 g/mol
0.01 G_PER_L CHEBI:33118
boric acid
21 Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
0.01 G_PER_L CHEBI:75213
sodium molybdate dihydrate
22 Biotin
C10H16N2O3S 244.316 g/mol
0.002 G_PER_L CHEBI:15956
biotin
23 Folic acid
C19H19N7O6 441.404 g/mol
0.002 G_PER_L CHEBI:27470
folic acid
24 Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
0.01 G_PER_L CHEBI:30961
pyridoxine hydrochloride
25 Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
0.005 G_PER_L CHEBI:49105
thiamine hydrochloride
26 Riboflavin
C17H20N4O6 376.369 g/mol
0.005 G_PER_L CHEBI:17015
riboflavin
27 Nicotinic acid
C6H5NO2 123.111 g/mol
0.005 G_PER_L CHEBI:15940
nicotinic acid
28 Calcium pantothenate
2C9H16NO5.Ca 476.536 g/mol
0.005 G_PER_L CHEBI:31345
Calcium pantothenate
29 Vitamin B12 0.0001 G_PER_L CHEBI:176843
vitamin B12
30 p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
0.005 G_PER_L CHEBI:30753
4-aminobenzoic acid
31 Lipoic acid
C8H14O2S2 206.332 g/mol
0.005 G_PER_L CHEBI:16494
lipoic acid

Preparation

  1. Mix components thoroughly. Bring to a boil for a few seconds, cool down under a N2-CO2 (4:1, v/v) gas mixture, seal with butyl rubber stoppers and autoclave. After cooling, add the following solutions (autoclaved or *filter-sterilized):
  2. Aseptically distribute the medium into culture vessels (e.g., 5 ml of the medium in the Hungate tubes) under a N2-CO2 (4:1, v/v) gas mixture and seal with butyl rubber stoppers. Add per liter the following solutions from anaerobic stocks (autoclaved and stored under a N2 gas atmosphere):
  3. Readjust pH to 7.5, if necessary.
  4. Dissolve nitrilotriacetic acid and adjust pH to 6.5 with KOH solution. Then proceed to add minerals. Adjust final pH to 7.0.

Target organisms

No target organisms are recorded.

Provenance

Merged from 1 source record.

TimestampCuratorActionNotes
2026-01-26T17:53:18.252975Z mediadive-import Imported from MediaDive Source: JCM, ID: J994
2026-03-13T03:08:54.492893Z data-quality-cleanup-v1.0 Data quality fixes Merged 3 duplicate ingredient(s)
2026-03-13T06:19:19.669845Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003343
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:33.707460+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "MBM MEDIUM 2" to "mbm_medium_2" for consistent matching
2026-06-05T07:41:13.371391+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 29 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:47:01.047324+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 4 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:05:42.310664+00:00 merge_recipes.py MERGED_RECIPES merged 1 source recipe(s) on fingerprint 5a66bd65f4c27bf5a4d9f9313cf0318d0478878369f16daae2a72772877230e6