Overview
- External ontology
- komodo.medium:1012 (source link unavailable) — DN BROTH
- Applications
-
- Microbial cultivation
- pH
- 7.2
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 1012 | DSMZ Medium: 1012 (mediadive.medium:1012) | Aerobic: No
Composition
flowchart LR
CultureMech_003523["dn_broth"]
ing0_HEPES_buffer["HEPES buffer"]
CultureMech_003523 --> ing0_HEPES_buffer
CHEBI_46756["CHEBI:46756"]
ing0_HEPES_buffer -.-> CHEBI_46756
ing1_MgCl2_x_6_H2O["MgCl2 x 6 H2O"]
CultureMech_003523 --> ing1_MgCl2_x_6_H2O
CHEBI_86345["CHEBI:86345"]
ing1_MgCl2_x_6_H2O -.-> CHEBI_86345
ing2_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003523 --> ing2_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing2_CaCl2_x_2_H2O -.-> CHEBI_86158
ing3_NaOH["NaOH"]
CultureMech_003523 --> ing3_NaOH
CHEBI_32145["CHEBI:32145"]
ing3_NaOH -.-> CHEBI_32145
Ingredients (4)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | HEPES buffer | 6 G_PER_L |
CHEBI:46756
HEPES
|
|
| 2 |
MgCl2 x 6 H2O
2Cl.6H2O.Mg 203.301 g/mol
|
0.6 G_PER_L |
CHEBI:86345
magnesium dichloride hexahydrate
|
|
| 3 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.3 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 4 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- dn_broth (CultureMech:003523)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-06-14T05:58:58.561437+00:00 | idnf-grounding-fix-v1.0 | Fixed ID_NOT_FOUND term groundings | Absent/obsolete CHEBI term ids fixed - 1 re-grounded to the correct CHEBI, 0 de-grounded (mixture or unverifiable). |
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1012 |
| 2026-02-03T11:19:12.160253 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1012 |
| 2026-03-13T03:09:24.939664Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:20.433878Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003523 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:33.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.568302+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "DN BROTH" to "dn_broth" for consistent matching |
| 2026-05-16T09:03:30.917578+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:58.797018+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 3 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:05:33.007972+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 1253f6fa17ce68c3912cc7cdbe192fe57e78a6060b554d3ab3c59b73a5b9543d |