Overview
- External ontology
- komodo.medium:1019 (source link unavailable) — ROUF'S medium
- Applications
-
- Microbial cultivation
- pH
- 7.1
- Notes
- pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 1019 | DSMZ Medium: 1019 (mediadive.medium:1019) | Aerobic: No
Composition
flowchart LR
CultureMech_003530["roufs_medium"]
ing0_Yeast_extract["Yeast extract"]
CultureMech_003530 --> ing0_Yeast_extract
ing1_Peptone["Peptone"]
CultureMech_003530 --> ing1_Peptone
ing2_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
CultureMech_003530 --> ing2_MgSO4_x_7_H2O
CHEBI_31795["CHEBI:31795"]
ing2_MgSO4_x_7_H2O -.-> CHEBI_31795
ing3_CaCl2_x_6_H2O["CaCl2 x 6 H2O"]
CultureMech_003530 --> ing3_CaCl2_x_6_H2O
CHEBI_91243["CHEBI:91243"]
ing3_CaCl2_x_6_H2O -.-> CHEBI_91243
ing4_Fe_NH4_citrate["Fe(NH4)citrate"]
CultureMech_003530 --> ing4_Fe_NH4_citrate
CHEBI_31604["CHEBI:31604"]
ing4_Fe_NH4_citrate -.-> CHEBI_31604
ing5_MnSO4_x_4_H2O["MnSO4 x 4 H2O"]
CultureMech_003530 --> ing5_MnSO4_x_4_H2O
CHEBI_86358["CHEBI:86358"]
ing5_MnSO4_x_4_H2O -.-> CHEBI_86358
ing6_FeCl3_x_4_H2O["FeCl3 x 4 H2O"]
CultureMech_003530 --> ing6_FeCl3_x_4_H2O
CHEBI_30808["CHEBI:30808"]
ing6_FeCl3_x_4_H2O -.-> CHEBI_30808
ing7_Agar["Agar"]
CultureMech_003530 --> ing7_Agar
CHEBI_2509["CHEBI:2509"]
ing7_Agar -.-> CHEBI_2509
ing8_Nitrilotriacetic_acid["Nitrilotriacetic acid"]
CultureMech_003530 --> ing8_Nitrilotriacetic_acid
CHEBI_44557["CHEBI:44557"]
ing8_Nitrilotriacetic_acid -.-> CHEBI_44557
ing9_CoCl2_x_6_H2O["CoCl2 x 6 H2O"]
CultureMech_003530 --> ing9_CoCl2_x_6_H2O
CHEBI_53503["CHEBI:53503"]
ing9_CoCl2_x_6_H2O -.-> CHEBI_53503
ing10_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
CultureMech_003530 --> ing10_CaCl2_x_2_H2O
CHEBI_86158["CHEBI:86158"]
ing10_CaCl2_x_2_H2O -.-> CHEBI_86158
ing11_FeSO4_x_7_H2O["FeSO4 x 7 H2O"]
CultureMech_003530 --> ing11_FeSO4_x_7_H2O
CHEBI_75836["CHEBI:75836"]
ing11_FeSO4_x_7_H2O -.-> CHEBI_75836
ing12_MnCl2_x_4_H2O["MnCl2 x 4 H2O"]
CultureMech_003530 --> ing12_MnCl2_x_4_H2O
CHEBI_86368["CHEBI:86368"]
ing12_MnCl2_x_4_H2O -.-> CHEBI_86368
ing13_NaCl["NaCl"]
CultureMech_003530 --> ing13_NaCl
CHEBI_26710["CHEBI:26710"]
ing13_NaCl -.-> CHEBI_26710
ing14_Na2MoO4_x_2_H2O["Na2MoO4 x 2 H2O"]
CultureMech_003530 --> ing14_Na2MoO4_x_2_H2O
CHEBI_75213["CHEBI:75213"]
ing14_Na2MoO4_x_2_H2O -.-> CHEBI_75213
ing15_Biotin["Biotin"]
CultureMech_003530 --> ing15_Biotin
CHEBI_15956["CHEBI:15956"]
ing15_Biotin -.-> CHEBI_15956
ing16_Folic_acid["Folic acid"]
CultureMech_003530 --> ing16_Folic_acid
CHEBI_27470["CHEBI:27470"]
ing16_Folic_acid -.-> CHEBI_27470
ing17_Pyridoxine_hydrochloride["Pyridoxine hydrochloride"]
CultureMech_003530 --> ing17_Pyridoxine_hydrochloride
CHEBI_30961["CHEBI:30961"]
ing17_Pyridoxine_hydrochloride -.-> CHEBI_30961
ing18_Thiamine_HCl["Thiamine HCl"]
CultureMech_003530 --> ing18_Thiamine_HCl
CHEBI_49105["CHEBI:49105"]
ing18_Thiamine_HCl -.-> CHEBI_49105
ing19_Riboflavin["Riboflavin"]
CultureMech_003530 --> ing19_Riboflavin
CHEBI_17015["CHEBI:17015"]
ing19_Riboflavin -.-> CHEBI_17015
ing20_Nicotinic_acid["Nicotinic acid"]
CultureMech_003530 --> ing20_Nicotinic_acid
CHEBI_15940["CHEBI:15940"]
ing20_Nicotinic_acid -.-> CHEBI_15940
ing21_Calcium_D_____pantothenate["Calcium D-(+)-pantothenate"]
CultureMech_003530 --> ing21_Calcium_D_____pantothenate
CHEBI_31345["CHEBI:31345"]
ing21_Calcium_D_____pantothenate -.-> CHEBI_31345
ing22_Vitamin_B12["Vitamin B12"]
CultureMech_003530 --> ing22_Vitamin_B12
CHEBI_176843["CHEBI:176843"]
ing22_Vitamin_B12 -.-> CHEBI_176843
ing23_p_Aminobenzoic_acid["p-Aminobenzoic acid"]
CultureMech_003530 --> ing23_p_Aminobenzoic_acid
CHEBI_30753["CHEBI:30753"]
ing23_p_Aminobenzoic_acid -.-> CHEBI_30753
ing24__DL__alpha_Lipoic_acid["(DL)-alpha-Lipoic acid"]
CultureMech_003530 --> ing24__DL__alpha_Lipoic_acid
CHEBI_16494["CHEBI:16494"]
ing24__DL__alpha_Lipoic_acid -.-> CHEBI_16494
ing25_NaOH["NaOH"]
CultureMech_003530 --> ing25_NaOH
CHEBI_32145["CHEBI:32145"]
ing25_NaOH -.-> CHEBI_32145
Ingredients (26)
| # | Ingredient | Concentration | Ontology | Notes |
|---|---|---|---|---|
| 1 | Yeast extract | 5 G_PER_L | ||
| 2 | Peptone | 5 G_PER_L | ||
| 3 |
MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
|
0.2 G_PER_L |
CHEBI:31795
magnesium sulfate heptahydrate
|
|
| 4 |
CaCl2 x 6 H2O
Ca.2Cl.6H2O 219.074 g/mol
|
0.05 G_PER_L |
CHEBI:91243
calcium chloride hexahydrate
|
|
| 5 | Fe(NH4)citrate | 0.015 G_PER_L |
CHEBI:31604
ferric ammonium citrate
|
|
| 6 |
MnSO4 x 4 H2O
4H2O.Mn.O4S 223.061 g/mol
|
0.05 G_PER_L |
CHEBI:86358
manganese(II) sulfate tetrahydrate
|
|
| 7 |
FeCl3 x 4 H2O
Cl3Fe 162.204 g/mol
|
0.01 G_PER_L |
CHEBI:30808
iron trichloride
|
|
| 8 | Agar | 17 G_PER_L |
CHEBI:2509
agar
|
(for solid medium) |
| 9 |
Nitrilotriacetic acid
C6H9NO6 191.139 g/mol
|
12.8 G_PER_L |
CHEBI:44557
nitrilotriacetic acid
|
|
| 10 |
CoCl2 x 6 H2O
2Cl.Co.6H2O 237.929 g/mol
|
0.17 G_PER_L |
CHEBI:53503
cobalt chloride hexahydrate
|
|
| 11 |
CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
|
0.1 G_PER_L |
CHEBI:86158
calcium chloride dihydrate
|
|
| 12 |
FeSO4 x 7 H2O
Fe.7H2O.O4S 278.013 g/mol
|
0.1 G_PER_L |
CHEBI:75836
iron(2+) sulfate heptahydrate
|
|
| 13 |
MnCl2 x 4 H2O
Cl2Mn.4H2O 197.904 g/mol
|
0.1 G_PER_L |
CHEBI:86368
manganese(II) chloride tetrahydrate
|
|
| 14 |
NaCl
Cl.Na 58.443 g/mol
|
0.1 G_PER_L |
CHEBI:26710
sodium chloride
|
|
| 15 |
Na2MoO4 x 2 H2O
2H2O.MoO4.2Na 241.946 g/mol
|
0.1 G_PER_L |
CHEBI:75213
sodium molybdate dihydrate
|
|
| 16 |
Biotin
C10H16N2O3S 244.316 g/mol
|
0.002 G_PER_L |
CHEBI:15956
biotin
|
|
| 17 |
Folic acid
C19H19N7O6 441.404 g/mol
|
0.002 G_PER_L |
CHEBI:27470
folic acid
|
|
| 18 |
Pyridoxine hydrochloride
C8H11NO3.HCl 205.641 g/mol
|
0.01 G_PER_L |
CHEBI:30961
pyridoxine hydrochloride
|
|
| 19 |
Thiamine HCl
C12H17N4OS.Cl.HCl 337.276 g/mol
|
0.005 G_PER_L |
CHEBI:49105
thiamine hydrochloride
|
|
| 20 |
Riboflavin
C17H20N4O6 376.369 g/mol
|
0.005 G_PER_L |
CHEBI:17015
riboflavin
|
|
| 21 |
Nicotinic acid
C6H5NO2 123.111 g/mol
|
0.005 G_PER_L |
CHEBI:15940
nicotinic acid
|
|
| 22 |
Calcium D-(+)-pantothenate
2C9H16NO5.Ca 476.536 g/mol
|
0.005 G_PER_L |
CHEBI:31345
Calcium pantothenate
|
|
| 23 | Vitamin B12 | 0.0001 G_PER_L |
CHEBI:176843
vitamin B12
|
|
| 24 |
p-Aminobenzoic acid
C7H7NO2 137.138 g/mol
|
0.005 G_PER_L |
CHEBI:30753
4-aminobenzoic acid
|
|
| 25 |
(DL)-alpha-Lipoic acid
C8H14O2S2 206.332 g/mol
|
0.005 G_PER_L |
CHEBI:16494
lipoic acid
|
|
| 26 |
NaOH
HO.Na 39.997 g/mol
|
variable VARIABLE |
CHEBI:32145
sodium hydroxide
|
pH adjustment (extracted from notes) |
Target organisms
No target organisms are recorded.
Provenance
Merged from 1 source record.
- KOMODO_1019_ROUF_S_medium (CultureMech:003530)
| Timestamp | Curator | Action | Notes |
|---|---|---|---|
| 2026-01-27T01:15:01.fZ | komodo-web-import | Imported from KOMODO web table | Source: KOMODO, ID: 1019 |
| 2026-02-03T11:19:17.044306 | dsmz-resolver-v1.0 | Enriched with DSMZ composition | Copied ingredients from DSMZ Medium 1019 |
| 2026-02-06T05:10:50.368970Z | invalid-chebi-removal | Removed 1 invalid CHEBI ID(s) | Removed CHEBI IDs with invalid format (7+ digits) from MicrobeMediaParam source data |
| 2026-03-13T03:09:36.876722Z | data-quality-cleanup-v1.0 | Data quality fixes | Added pH buffer: NaOH |
| 2026-03-13T06:19:20.467458Z | culturemech-id-assigner-v1.0 | Assigned CultureMech ID | Assigned stable identifier: CultureMech:003530 |
| 2026-03-14T00:00:00+00:00 | schema-defaulter-v1.0 | Applied schema defaults and normalizations | Added default concentration for ingredient: NaOH |
| 2026-03-17T06:30:48.000000Z | mediaingredientmech-enrichment-v1.0 | Added MediaIngredientMech links | Linked ingredients to MediaIngredientMech identifiers |
| 2026-03-17T19:45:34.606773+00:00 | normalize_media_names_to_snake_case.py | NAME_NORMALIZED | Normalized name from "ROUF'S medium" to "roufs_medium" for consistent matching |
| 2026-05-16T09:02:59.625773+00:00 | migrate_legacy_fields.py | MIGRATED_LEGACY_FIELDS | curation_history.date->timestamp=1 |
| 2026-06-05T07:40:18.147370+00:00 | mim-legacy-to-chebi-migration-v1.0 | Refreshed legacy MediaIngredientMech links to CHEBI keying | Replaced 23 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme. |
| 2026-08-06T03:04:37.890443+00:00 | merge_recipes.py | MERGED_RECIPES | merged 1 source recipe(s) on fingerprint 468f17f25add2552f2ae22fb7d1b14530e53f6ee84bc843213f390c3dfc09647 |