pyg_medium_modified

CultureMech:003608 bacterial COMPLEX LIQUID
Original name: PYG-MEDIUM (modified)

Overview

External ontology
komodo.medium:104 (source link unavailable) — PYG-MEDIUM (modified)
Applications
  • Microbial cultivation
Synonyms
  • {'name': 'medium_104b_modified_for_dsm_13275', 'source': 'KOMODO', 'source_id': 'komodo.medium:104b_13275', 'original_category': 'bacterial'}
pH
7.2
Notes
pH buffer: NaOH | Source: KOMODO ModelSEED | ID: 104 | DSMZ Medium: 104 (mediadive.medium:104) | Aerobic: Yes

Composition

flowchart LR
    CultureMech_003608["pyg_medium_modified"]
    ing0_Trypticase_peptone["Trypticase peptone"]
    CultureMech_003608 --> ing0_Trypticase_peptone
    ing1_Meat_peptone["Meat peptone"]
    CultureMech_003608 --> ing1_Meat_peptone
    ing2_Yeast_extract["Yeast extract"]
    CultureMech_003608 --> ing2_Yeast_extract
    ing3_NaCl["NaCl"]
    CultureMech_003608 --> ing3_NaCl
    CHEBI_26710["CHEBI:26710"]
    ing3_NaCl -.-> CHEBI_26710
    ing4_Sodium_resazurin["Sodium resazurin"]
    CultureMech_003608 --> ing4_Sodium_resazurin
    CHEBI_8806["CHEBI:8806"]
    ing4_Sodium_resazurin -.-> CHEBI_8806
    ing5_L_Cysteine_HCl_x_H2O["L-Cysteine HCl x H2O"]
    CultureMech_003608 --> ing5_L_Cysteine_HCl_x_H2O
    CHEBI_91248["CHEBI:91248"]
    ing5_L_Cysteine_HCl_x_H2O -.-> CHEBI_91248
    ing6_D_Glucose["D-Glucose"]
    CultureMech_003608 --> ing6_D_Glucose
    CHEBI_17634["CHEBI:17634"]
    ing6_D_Glucose -.-> CHEBI_17634
    ing7_Na2S2O3_x_5_H2O["Na2S2O3 x 5 H2O"]
    CultureMech_003608 --> ing7_Na2S2O3_x_5_H2O
    CHEBI_32150["CHEBI:32150"]
    ing7_Na2S2O3_x_5_H2O -.-> CHEBI_32150
    ing8_CaCl2_x_2_H2O["CaCl2 x 2 H2O"]
    CultureMech_003608 --> ing8_CaCl2_x_2_H2O
    CHEBI_86158["CHEBI:86158"]
    ing8_CaCl2_x_2_H2O -.-> CHEBI_86158
    ing9_MgSO4_x_7_H2O["MgSO4 x 7 H2O"]
    CultureMech_003608 --> ing9_MgSO4_x_7_H2O
    CHEBI_31795["CHEBI:31795"]
    ing9_MgSO4_x_7_H2O -.-> CHEBI_31795
    ing10_K2HPO4["K2HPO4"]
    CultureMech_003608 --> ing10_K2HPO4
    CHEBI_131527["CHEBI:131527"]
    ing10_K2HPO4 -.-> CHEBI_131527
    ing11_KH2PO4["KH2PO4"]
    CultureMech_003608 --> ing11_KH2PO4
    CHEBI_63036["CHEBI:63036"]
    ing11_KH2PO4 -.-> CHEBI_63036
    ing12_NaHCO3["NaHCO3"]
    CultureMech_003608 --> ing12_NaHCO3
    CHEBI_32139["CHEBI:32139"]
    ing12_NaHCO3 -.-> CHEBI_32139
    ing13_NaOH["NaOH"]
    CultureMech_003608 --> ing13_NaOH
    CHEBI_32145["CHEBI:32145"]
    ing13_NaOH -.-> CHEBI_32145

Ingredients (14)

# Ingredient Concentration Ontology Notes
1 Trypticase peptone 5 G_PER_L
2 Meat peptone 5 G_PER_L
3 Yeast extract 10 G_PER_L
4 NaCl
Cl.Na 58.443 g/mol
10.0 G_PER_L CHEBI:26710
sodium chloride
 [Merged 2 duplicates: 8.0, 2.0]
5 Sodium resazurin
C12H7NO4 229.191 g/mol
0.0005 G_PER_L CHEBI:8806
Resazurin
6 L-Cysteine HCl x H2O
C3H7NO2S.H2O.HCl 175.637 g/mol
0.5 G_PER_L CHEBI:91248
L-cysteine hydrochloride hydrate
7 D-Glucose
C6H12O6 180.156 g/mol
1 G_PER_L CHEBI:17634
D-glucose
8 Na2S2O3 x 5 H2O
5H2O.2Na.O3S2 248.186 g/mol
2.5 G_PER_L CHEBI:32150
sodium thiosulfate pentahydrate
9 CaCl2 x 2 H2O
Ca.2Cl.2H2O 147.014 g/mol
0.25 G_PER_L CHEBI:86158
calcium chloride dihydrate
10 MgSO4 x 7 H2O
7H2O.Mg.O4S 246.473 g/mol
0.5 G_PER_L CHEBI:31795
magnesium sulfate heptahydrate
11 K2HPO4
HO4P.2K 174.174 g/mol
1 G_PER_L CHEBI:131527
dipotassium hydrogen phosphate
12 KH2PO4
H2O4P.K 136.084 g/mol
1 G_PER_L CHEBI:63036
potassium dihydrogen phosphate
13 NaHCO3
CHO3.Na 84.006 g/mol
10 G_PER_L CHEBI:32139
sodium hydrogencarbonate
14 NaOH
HO.Na 39.997 g/mol
variable VARIABLE CHEBI:32145
sodium hydroxide
 pH adjustment (extracted from notes)

Target organisms

No target organisms are recorded.

Provenance

Merged from 2 source records.

TimestampCuratorActionNotes
2026-01-27T01:15:01.fZ komodo-web-import Imported from KOMODO web table Source: KOMODO, ID: 104
2026-02-03T11:19:00.925352 dsmz-resolver-v1.0 Enriched with DSMZ composition Copied ingredients from DSMZ Medium 104
2026-03-13T03:08:54.197974Z data-quality-cleanup-v1.0 Data quality fixes Merged 1 duplicate ingredient(s); Added pH buffer: NaOH
2026-03-13T06:19:21.000720Z culturemech-id-assigner-v1.0 Assigned CultureMech ID Assigned stable identifier: CultureMech:003608
2026-03-14T00:00:00+00:00 schema-defaulter-v1.0 Applied schema defaults and normalizations Added default concentration for ingredient: NaOH
2026-03-17T06:29:58.000000Z mediaingredientmech-enrichment-v1.0 Added MediaIngredientMech links Linked ingredients to MediaIngredientMech identifiers
2026-03-17T19:45:35.199390+00:00 normalize_media_names_to_snake_case.py NAME_NORMALIZED Normalized name from "PYG-MEDIUM (modified)" to "pyg_medium_modified" for consistent matching
2026-05-16T09:02:59.770546+00:00 migrate_legacy_fields.py MIGRATED_LEGACY_FIELDS curation_history.date->timestamp=1
2026-06-05T07:40:18.485120+00:00 mim-legacy-to-chebi-migration-v1.0 Refreshed legacy MediaIngredientMech links to CHEBI keying Replaced 10 legacy mediaingredientmech_term link(s) with mediaingredientmech_chebi_term (id-safe: ingredient's own CHEBI + label). MIM deprecated the MediaIngredientMech:NNNNNN id scheme.
2026-06-10T07:46:43.325866+00:00 primary-term-label-canonicalization-v1.0 Canonicalized primary term label to OBO canonical (OAK) 2 primary term(s); ingredient name resolved by OAK sqlite:obo:chebi.
2026-08-06T03:04:38.288739+00:00 recipe-merger Merged 2 duplicate recipes into canonical record Sources: KOMODO_104_PYG-MEDIUM_modified, medium_104b_modified_for_dsm_13275
2026-08-06T03:04:38.288787+00:00 merge_recipes.py MERGED_RECIPES merged 2 source recipe(s) on fingerprint 847326560eaa063c1b35bff09b99d23d9edabcecb03ea1f59a5b52261cd2d6e5